دورية أكاديمية
أعرض في EDS

Dendritic cell-targeted porcine reproductive and respiratory syndrome virus (PRRSV) antigens adjuvanted with polyinosinic-polycytidylic acid (poly (I:C)) induced non-protective immune responses against heterologous type 2 PRRSV challenge in pigs.

التفاصيل البيبلوغرافية
العنوان: Dendritic cell-targeted porcine reproductive and respiratory syndrome virus (PRRSV) antigens adjuvanted with polyinosinic-polycytidylic acid (poly (I:C)) induced non-protective immune responses against heterologous type 2 PRRSV challenge in pigs.
المؤلفون: Subramaniam S; Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24060, USA., Piñeyro P; Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University College of Veterinary Medicine, Ames, IA 50011, USA., Derscheid RJ; Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University College of Veterinary Medicine, Ames, IA 50011, USA., Madson DM; Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University College of Veterinary Medicine, Ames, IA 50011, USA., Magstadt DR; Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University College of Veterinary Medicine, Ames, IA 50011, USA., Meng XJ; Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24060, USA. Electronic address: xjmeng@vt.edu.
المصدر: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2017 Aug; Vol. 190, pp. 18-25. Date of Electronic Publication: 2017 Jul 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Scientific Country of Publication: Netherlands NLM ID: 8002006 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2534 (Electronic) Linking ISSN: 01652427 NLM ISO Abbreviation: Vet Immunol Immunopathol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] Elsevier Scientific.
مواضيع طبية MeSH: Antigens, Viral/*immunology , Porcine Reproductive and Respiratory Syndrome/*immunology , Porcine respiratory and reproductive syndrome virus/*immunology , Viral Vaccines/*pharmacology, Adjuvants, Immunologic/pharmacology ; Animals ; Dendritic Cells/immunology ; Dendritic Cells/virology ; Poly I-C/pharmacology ; Porcine Reproductive and Respiratory Syndrome/prevention & control ; Swine ; Viral Vaccines/immunology
مستخلص: Porcine Reproductive and Respiratory Syndrome (PRRS) is an economically important swine viral disease worldwide. Current modified live-attenuated vaccines are ineffective against heterologous strains of PRRS virus (PRRSV) circulating in the field. In this study, we evaluated three dendritic cell (DC)-targeted vaccine candidates for their protective efficacy against heterologous PRRSV challenge. Ectodomain regions of DNA-shuffled structural proteins GP3, GP4, GP5 and M of PRRSV were fused together to form the vaccine antigen which was in turn fused with one of three recombinant antibodies each specific to a DC receptor: DC-SIGN, Langerin, and DEC205. The recombinant antibody-fused vaccine antigens were co-administered with polyinosinic-polycytidylic acid (poly (I:C)) adjuvant and subsequently challenged with a heterologous type 2 PRRSV strain (NADC20) in pigs. Our results demonstrate that pigs in DC-SIGN- and DEC205-targeted, but not Langerin- and non-targeted, vaccine groups showed significant IFN-γ- and IL-4-specific CD4T cell immune responses against the vaccine antigen in 7days post-challenge. Pigs in DC-SIGN- and Langerin-targeted vaccine groups showed greatly reduced IgG responses as compared to the DEC205- and non-targeted vaccine groups. The immune responses induced by DC-targeted vaccines did not reduce viremia and lung pathological lesions in type 2 PRRSV-challenged pigs. In contrast, pigs in Langerin-targeted vaccine group showed significantly increased serum viral titers and viral antigen in lung tissues at 7 and 14days post-challenge respectively. In conclusion, specific targeting of PRRSV antigen through DC-SIGN or DEC205 or Langerin-specific antibodies in the presence of poly (I:C) adjuvant induced immune responses that failed to protect pigs against heterologous type 2 PRRSV challenge.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Dendritic cell targeting; Porcine DC-SIGN; Porcine DEC205; Porcine langerin; Porcine reproductive and respiratory syndrome virus (PRRSV); Subunit vaccine
المشرفين على المادة: 0 (Adjuvants, Immunologic)
0 (Antigens, Viral)
0 (Viral Vaccines)
O84C90HH2L (Poly I-C)
تواريخ الأحداث: Date Created: 20170806 Date Completed: 20171226 Latest Revision: 20171226
رمز التحديث: 20221213
DOI: 10.1016/j.vetimm.2017.07.003
PMID: 28778318
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2534
DOI:10.1016/j.vetimm.2017.07.003