دورية أكاديمية
Insulin Influences LPS-Induced TNF-α and IL-6 Release Through Distinct Pathways in Mouse Macrophages from Different Compartments.
| العنوان: | Insulin Influences LPS-Induced TNF-α and IL-6 Release Through Distinct Pathways in Mouse Macrophages from Different Compartments. |
|---|---|
| المؤلفون: | Tessaro FHG, Ayala TS, Nolasco EL, Bella LM, Martins JO |
| المصدر: | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2017; Vol. 42 (5), pp. 2093-2104. Date of Electronic Publication: 2017 Aug 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Physiol Biochem Press GmbH & Co KG Country of Publication: Germany NLM ID: 9113221 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1421-9778 (Electronic) Linking ISSN: 10158987 NLM ISO Abbreviation: Cell Physiol Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Düsseldorf, Germany : Cell Physiol Biochem Press GmbH & Co KG Original Publication: Basel ; New York : S. Karger, 1991-2018. |
| مواضيع طبية MeSH: | Insulin/*pharmacology , Interleukin-6/*metabolism , Lipopolysaccharides/*toxicity , Macrophages/*drug effects , Tumor Necrosis Factor-alpha/*metabolism, Androstadienes/pharmacology ; Animals ; Bone Marrow Cells/cytology ; Cell Survival/drug effects ; Cells, Cultured ; Diabetes Mellitus, Experimental/chemically induced ; Diabetes Mellitus, Experimental/metabolism ; Diabetes Mellitus, Experimental/pathology ; Flavonoids/pharmacology ; Macrophages/cytology ; Macrophages/metabolism ; Macrophages, Alveolar/cytology ; Macrophages, Alveolar/drug effects ; Macrophages, Alveolar/metabolism ; Macrophages, Peritoneal/cytology ; Macrophages, Peritoneal/drug effects ; Macrophages, Peritoneal/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mitogen-Activated Protein Kinase 1/antagonists & inhibitors ; Mitogen-Activated Protein Kinase 1/metabolism ; Mitogen-Activated Protein Kinase 3/antagonists & inhibitors ; Mitogen-Activated Protein Kinase 3/metabolism ; Signal Transduction/drug effects ; Wortmannin |
| مستخلص: | Background/aims: Diabetic subjects are more susceptible to infections, which is partially due to insulin deficiency and hyperglycemia. We hypothesized that insulin influences cytokine release by macrophages from diabetic C57BL/6 mice stimulated with lipopolysaccharides (LPS). Methods: Bone marrow-derived macrophages (BMDM) and tissue-specific macrophages from diabetic (alloxan 60 mg/kg, i.v.) male C57BL/6 mice were stimulated by LPS (100 ng/mL) and/or treated by insulin (1 mU/mL). Results: Using BMDM from diabetic mice, we showed that LPS induced an increase in TNF-α and IL-6 release and p38, SAPK/JNK, ERK 1/2, and Akt (308-Thr and 473-Ser) phosphorylation but not in PKCα/β II and delta. Insulin increased TNF-α and IL-6 secretion in LPS-stimulated macrophages as well as p-p38, p-SAPK/JNK, p-ERK 1/2, p-PI3K (p55) and p-Akt (473-Ser) expression. Furthermore, PI3-kinase inhibition by wortmannin decreased TNF-α release, and inhibition by LY294002 decreased both TNF-α and IL-6 levels after LPS-insulin treatment. PD98059, which inhibits the ERK upstream activators MAPK kinase (MKK) 1 and MKK2, reduced the effect promoted by insulin in BMDM stimulated by LPS In tissue-specific macrophages, insulin reduced LPS-induced TNF-α, IL-6 and IL-1β secretion in alveolar and peritoneal macrophages. Conclusion: These data suggest that insulin through the modulation of PI3-kinase and ERK 1/2 pathways drive different responses in macrophages, thereby enhancing our understanding of the plasticity of these cells. (© 2017 The Author(s). Published by S. Karger AG, Basel.) |
| فهرسة مساهمة: | Keywords: Akt; Alloxan; Diabetes; ERK; Macrophage; PI3K |
| المشرفين على المادة: | 0 (Androstadienes) 0 (Flavonoids) 0 (Insulin) 0 (Interleukin-6) 0 (Lipopolysaccharides) 0 (Tumor Necrosis Factor-alpha) EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1) EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3) SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one) XVA4O219QW (Wortmannin) |
| تواريخ الأحداث: | Date Created: 20170816 Date Completed: 20171116 Latest Revision: 20220317 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1159/000479904 |
| PMID: | 28810254 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1421-9778 |
|---|---|
| DOI: | 10.1159/000479904 |