دورية أكاديمية
Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA.
العنوان: | Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA. |
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المؤلفون: | Németh A; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Orgovan N; Institute of Technical Physics and Materials Science, Hungarian Academy of Sciences, Budapest, 1121, Hungary., Sódar BW; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Osteikoetxea X; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Pálóczi K; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Szabó-Taylor KÉ; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Vukman KV; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Kittel Á; Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary., Turiák L; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, 1117, Hungary., Wiener Z; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Tóth S; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Drahos L; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, 1117, Hungary., Vékey K, Horvath R, Buzás EI; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary. edit.buzas@gmail.com. |
المصدر: | Scientific reports [Sci Rep] 2017 Aug 15; Vol. 7 (1), pp. 8202. Date of Electronic Publication: 2017 Aug 15. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
مواضيع طبية MeSH: | Anti-Bacterial Agents/*pharmacology , DNA/*metabolism , Exosomes/*metabolism , Extracellular Vesicles/*metabolism , Intracellular Membranes/*metabolism, Apoptosis/drug effects ; Cell Line ; Cell Survival/drug effects ; Ciprofloxacin/pharmacology ; DNA, Mitochondrial ; Humans ; Jurkat Cells |
مستخلص: | Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibiotic ciprofloxacin on the released extracellular vesicles. Ciprofloxacin is widely used in humans against bacterial infections as well as in cell cultures against Mycoplasma contamination. However, ciprofloxacin is an inducer of oxidative stress and mitochondrial dysfunction of mammalian cells. Unexpectedly, here we found that ciprofloxacin induced the release of both DNA (mitochondrial and chromosomal sequences) and DNA-binding proteins on the exofacial surfaces of small extracellular vesicles referred to in this paper as exosomes. Furthermore, a label-free optical biosensor analysis revealed DNA-dependent binding of exosomes to fibronectin. DNA release on the surface of exosomes was not affected any further by cellular activation or apoptosis induction. Our results reveal for the first time that prolonged low-dose ciprofloxacin exposure leads to the release of DNA associated with the external surface of exosomes. |
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المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (DNA, Mitochondrial) 5E8K9I0O4U (Ciprofloxacin) 9007-49-2 (DNA) |
تواريخ الأحداث: | Date Created: 20170817 Date Completed: 20190307 Latest Revision: 20190307 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC5557920 |
DOI: | 10.1038/s41598-017-08392-1 |
PMID: | 28811610 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2045-2322 |
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DOI: | 10.1038/s41598-017-08392-1 |