دورية أكاديمية
أعرض في EDS

Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA.

التفاصيل البيبلوغرافية
العنوان: Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA.
المؤلفون: Németh A; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Orgovan N; Institute of Technical Physics and Materials Science, Hungarian Academy of Sciences, Budapest, 1121, Hungary., Sódar BW; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Osteikoetxea X; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Pálóczi K; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Szabó-Taylor KÉ; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Vukman KV; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Kittel Á; Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary., Turiák L; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, 1117, Hungary., Wiener Z; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Tóth S; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary., Drahos L; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, 1117, Hungary., Vékey K, Horvath R, Buzás EI; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, 1085, Hungary. edit.buzas@gmail.com.
المصدر: Scientific reports [Sci Rep] 2017 Aug 15; Vol. 7 (1), pp. 8202. Date of Electronic Publication: 2017 Aug 15.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Anti-Bacterial Agents/*pharmacology , DNA/*metabolism , Exosomes/*metabolism , Extracellular Vesicles/*metabolism , Intracellular Membranes/*metabolism, Apoptosis/drug effects ; Cell Line ; Cell Survival/drug effects ; Ciprofloxacin/pharmacology ; DNA, Mitochondrial ; Humans ; Jurkat Cells
مستخلص: Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibiotic ciprofloxacin on the released extracellular vesicles. Ciprofloxacin is widely used in humans against bacterial infections as well as in cell cultures against Mycoplasma contamination. However, ciprofloxacin is an inducer of oxidative stress and mitochondrial dysfunction of mammalian cells. Unexpectedly, here we found that ciprofloxacin induced the release of both DNA (mitochondrial and chromosomal sequences) and DNA-binding proteins on the exofacial surfaces of small extracellular vesicles referred to in this paper as exosomes. Furthermore, a label-free optical biosensor analysis revealed DNA-dependent binding of exosomes to fibronectin. DNA release on the surface of exosomes was not affected any further by cellular activation or apoptosis induction. Our results reveal for the first time that prolonged low-dose ciprofloxacin exposure leads to the release of DNA associated with the external surface of exosomes.
References: Nat Commun. 2015 Oct 07;6:8472. (PMID: 26442449)
PLoS One. 2007 Apr 25;2(4):e396. (PMID: 17460763)
J Proteomics. 2011 Sep 6;74(10):2025-33. (PMID: 21635979)
J Mol Cell Biol. 2013 Aug;5(4):227-38. (PMID: 23580760)
Mol Cell Biol. 2008 Aug;28(16):4975-87. (PMID: 18541666)
Appl Environ Microbiol. 2014 Jun;80(11):3469-83. (PMID: 24657872)
Oncotarget. 2017 Jan 3;8(1):1416-1428. (PMID: 27902458)
Antimicrob Agents Chemother. 2003 Mar;47(3):1161-4. (PMID: 12604563)
PLoS One. 2012;7(12):e44366. (PMID: 23284605)
J Proteomics. 2011 Jun 10;74(7):942-7. (PMID: 21256993)
Nat Cell Biol. 2007 Jun;9(6):654-9. (PMID: 17486113)
Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E968-77. (PMID: 26858453)
Leukemia. 2006 May;20(5):847-56. (PMID: 16453000)
Cell. 2016 Jun 30;166(1):88-101. (PMID: 27293190)
J Extracell Vesicles. 2015 May 14;4:27066. (PMID: 25979354)
Cell Mol Life Sci. 2014 Oct;71(20):4055-67. (PMID: 24705984)
Org Biomol Chem. 2015 Oct 14;13(38):9775-82. (PMID: 26264754)
Antimicrob Agents Chemother. 1998 Aug;42(8):1923-30. (PMID: 9687385)
J Immunol. 2017 Feb 15;198(4):1649-1659. (PMID: 28069806)
EMBO J. 2014 Oct 1;33(19):2142-56. (PMID: 25107473)
Sci Transl Med. 2013 Jul 3;5(192):192ra85. (PMID: 23825301)
Protein Cell. 2016 Jan;7(1):11-6. (PMID: 26498951)
Cancer Sci. 2003 Mar;94(3):297-301. (PMID: 12824925)
Proc Natl Acad Sci U S A. 2001 May 22;98(11):6407-11. (PMID: 11353826)
J Immunol. 2010 May 1;184(9):4827-41. (PMID: 20335530)
J Chemother. 2002 Feb;14(1):3-12. (PMID: 11892896)
Clin Exp Otorhinolaryngol. 2015 Sep;8(3):211-7. (PMID: 26330914)
Nat Rev Rheumatol. 2014 Jun;10(6):356-64. (PMID: 24535546)
Exp Cell Res. 2014 Nov 1;328(2):388-400. (PMID: 25194507)
PLoS One. 2012;7(11):e49726. (PMID: 23185418)
J Physiol Pharmacol. 2010 Apr;61(2):233-9. (PMID: 20436225)
Cell Res. 2014 Jun;24(6):766-9. (PMID: 24710597)
Nucleic Acids Res. 2001 May 1;29(9):e45. (PMID: 11328886)
Blood. 1999 Jun 1;93(11):3956-63. (PMID: 10339505)
Biophys J. 2006 Sep 1;91(5):1925-40. (PMID: 16766609)
Sci Rep. 2014 Feb 07;4:4034. (PMID: 24503534)
Oncogene. 2013 Oct 31;32(44):5201-9. (PMID: 23222712)
Blood. 2014 Oct 2;124(14):2173-83. (PMID: 25082876)
Prostate. 2014 Oct;74(14 ):1379-90. (PMID: 25111183)
J Extracell Vesicles. 2014 Sep 18;3:null. (PMID: 25317274)
Kidney Int. 2010 Jul;78(2):191-9. (PMID: 20428099)
Nat Biotechnol. 2008 Dec;26(12):1367-72. (PMID: 19029910)
J Antimicrob Chemother. 2003 May;51 Suppl 1:29-35. (PMID: 12702701)
J Leukoc Biol. 2003 Sep;74(3):456-63. (PMID: 12949250)
PLoS One. 2012;7(4):e34653. (PMID: 22506041)
Trends Microbiol. 2016 Jul;24(7):525-34. (PMID: 27040918)
Am J Pathol. 2012 Nov;181(5):1573-84. (PMID: 23022210)
Am J Physiol Renal Physiol. 2014 Dec 1;307(11):F1227-37. (PMID: 25339697)
Antimicrob Agents Chemother. 2006 May;50(5):1664-71. (PMID: 16641433)
Nat Rev Immunol. 2009 Aug;9(8):581-93. (PMID: 19498381)
Nat Commun. 2011 Feb 01;2:180. (PMID: 21285958)
J Neural Transm (Vienna). 2010 Jan;117(1):1-4. (PMID: 19680595)
Nat Cell Biol. 2008 Dec;10(12):1470-6. (PMID: 19011622)
PLoS One. 2015 Mar 23;10(3):e0121184. (PMID: 25798862)
J Biol Chem. 2014 Feb 14;289(7):3869-75. (PMID: 24398677)
ScientificWorldJournal. 2002 Jun 07;2:1551-4. (PMID: 12806139)
PLoS One. 2014 Aug 18;9(8):e105200. (PMID: 25133686)
Nepal Med Coll J. 2009 Sep;11(3):147-51. (PMID: 20334059)
Cytometry A. 2008 Mar;73(3):246-53. (PMID: 18163469)
J Immunol. 2010 Nov 1;185(9):5140-9. (PMID: 20870937)
PLoS One. 2016 Sep 29;11(9):e0163665. (PMID: 27684368)
Nature. 2010 Mar 4;464(7285):104-7. (PMID: 20203610)
Cell Mol Life Sci. 2011 Aug;68(16):2667-88. (PMID: 21560073)
J Extracell Vesicles. 2013 Sep 12;2:null. (PMID: 24223256)
Blood. 2011 Jan 27;117(4):e39-48. (PMID: 21041717)
Exp Cell Res. 2009 Mar 10;315(5):760-8. (PMID: 19146850)
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (DNA, Mitochondrial)
5E8K9I0O4U (Ciprofloxacin)
9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20170817 Date Completed: 20190307 Latest Revision: 20190307
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC5557920
DOI: 10.1038/s41598-017-08392-1
PMID: 28811610
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-017-08392-1