دورية أكاديمية
أعرض في EDS

The influence of physical activity in the anti-tumor immune response in experimental breast tumor.

التفاصيل البيبلوغرافية
العنوان: The influence of physical activity in the anti-tumor immune response in experimental breast tumor.
المؤلفون: Bianco TM; Oncology Research Institute (Instituto de Pesquisa em Oncologia-IPON), Federal University of the Triângulo Mineiro (UFTM), Brazil., Abdalla DR; Oncology Research Institute (Instituto de Pesquisa em Oncologia-IPON), Federal University of the Triângulo Mineiro (UFTM), Brazil., Desidério CS; Oncology Research Institute (Instituto de Pesquisa em Oncologia-IPON), Federal University of the Triângulo Mineiro (UFTM), Brazil., Thys S; Laboratory of Cell Biology and Histology, University of Antwerp, Belgium., Simoens C; Laboratory of Cell Biology and Histology, University of Antwerp, Belgium., Bogers JP; Laboratory of Cell Biology and Histology, University of Antwerp, Belgium., Murta EFC; Oncology Research Institute (IPON)/Discipline of Gynecology and Obstetrics, UFTM, Brazil., Michelin MA; Oncology Research Institute (IPON)/Discipline of Immunology, UFTM, Uberaba, Minas Gerais, Brazil. Electronic address: michelinimuno@icbn.uftm.edu.br.
المصدر: Immunology letters [Immunol Lett] 2017 Oct; Vol. 190, pp. 148-158. Date of Electronic Publication: 2017 Aug 14.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7910006 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0542 (Electronic) Linking ISSN: 01652478 NLM ISO Abbreviation: Immunol Lett Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
مواضيع طبية MeSH: Breast Neoplasms/*immunology , Dendritic Cells/*immunology , Exercise/*physiology , Lymphocytes, Tumor-Infiltrating/*immunology , Th1 Cells/*immunology, Animals ; Cell Line, Tumor ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Immunity, Innate ; Lymphocyte Activation ; Mice ; Mice, Inbred BALB C ; Neoplasms, Experimental ; Spleen/immunology ; Teaching ; Transcription Factors/genetics ; Transcription Factors/metabolism
مستخلص: This study aimed to investigate the influence of physical activity in innate immunity to conduce to an effective antitumoral immune response analyzing the phenotype and activation status of infiltrating cells. We analysed the intracellular cytokines and the transcription factors of tumor infiltrating lymphocytes (TILS) and spleen leukocytes. The Nos2 gene expression was evaluated in spleen cells and futhermore the ROS production was measured and spleen cells; another cell evaluated was dendritic cells (TIDCs), their cytokines expression and membrane molecules; finally to understood the results obtained, we analysed the dendritic cells obtained from bone marrow. Were used female Balb/c mice divided into 4 groups: two controls without tumor, sedentary (GI) and trained (GII) and two groups with tumor, sedentary (GIII) or trained (GIV). The physical activity (PA) was realized acoording swimming protocol. Tumor was induced by injection of 4T1 cells. All experiments were performed in biological triplicate. After the experimental period, the tumor was removed and the cells were identified by flow cytometry with labeling to CD4, CD8, CD11c, CD11b, CD80, CD86 and Ia, and intracelular staining IL-10, IL-12, TNF-α, IFN-γ, IL-17, Tbet, GATA3, RORγt and FoxP3. The bone marrow of the animals was obtained to analyse the derivated DCs by flow cytometry and culture cells to obtain the supernatant to measure the cytokines. Our results demonstrated that the PA inhibit the tumoral growth although not to change the number of TILS, but reduced expression of GATA-3, ROR-γT, related with poor prognosis, and TNF-α intracellular; however occur one significantly reduction in TIDCS, but these cells expressed more co-stimulatory and presentation molecules. Furthermore, we observed that the induced PA stimulated the gene expression of Tbet and the production of inflammatory cytokines suggesting an increase of Th1 systemic response. The results evaluating the systemic influence in DCs showed that the PA improve significantly the number of those cells in bone marrow as well the number of co-stimulatory molecules. Therefore, we could conclude that PA influence the innate immunity by interfering to promote in process of maturation of DCs both in tumor and systemically, that by its turn promote a modification in acquired immune cells, representing by T helper to induce an important alteration transcription factors that are responsible to maintain a suppressive microenviroment, and thereby, allowing the latter cells can thus activate antitumor immune response. The PA was able improve the Th1 systemic response by enhance to Tbet gene expression, promote a slightly increased of Th1-type cytokines and decrease Gata3 and Foxp3 gene expression in which can inhibit the Th1 immune response.
(Copyright © 2017 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Antitumoral immune response; Dendritic cells; Helper lymphocytes; Transcription factors
المشرفين على المادة: 0 (Transcription Factors)
تواريخ الأحداث: Date Created: 20170819 Date Completed: 20180712 Latest Revision: 20180720
رمز التحديث: 20240829
DOI: 10.1016/j.imlet.2017.08.007
PMID: 28818640
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0542
DOI:10.1016/j.imlet.2017.08.007