دورية أكاديمية
أعرض في EDS

The dual role of group V secretory phospholipase A 2 in pancreatic β-cells.

التفاصيل البيبلوغرافية
العنوان: The dual role of group V secretory phospholipase A 2 in pancreatic β-cells.
المؤلفون: Shridas P; Saha Cardiovascular Research Center, University of Kentucky Medical Center, Lexington, KY, 40536, USA. pshri2@uky.edu.; Departments of Internal Medicine, University of Kentucky Medical Center, Lexington, KY, 40536, USA. pshri2@uky.edu., Noffsinger VP; Saha Cardiovascular Research Center, University of Kentucky Medical Center, Lexington, KY, 40536, USA.; Departments of Internal Medicine, University of Kentucky Medical Center, Lexington, KY, 40536, USA., Trumbauer AC; Saha Cardiovascular Research Center, University of Kentucky Medical Center, Lexington, KY, 40536, USA., Webb NR; Saha Cardiovascular Research Center, University of Kentucky Medical Center, Lexington, KY, 40536, USA.; Pharmacology and Nutritional Sciences, Division of Nutritional Sciences, University of Kentucky Medical Center, Lexington, KY, 40536, USA.
المصدر: Endocrine [Endocrine] 2017 Oct; Vol. 58 (1), pp. 47-58. Date of Electronic Publication: 2017 Aug 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Humana Press Country of Publication: United States NLM ID: 9434444 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0100 (Electronic) Linking ISSN: 1355008X NLM ISO Abbreviation: Endocrine Subsets: MEDLINE
أسماء مطبوعة: Publication: Feb. 1996- : Totowa, NJ : Humana Press
Original Publication: Houndsmills, Basingstoke, Hants, UK : Macmillan Press, c1994-
مواضيع طبية MeSH: Group V Phospholipases A2/*metabolism , Insulin-Secreting Cells/*metabolism, Animals ; Arachidonic Acid/metabolism ; Cell Line ; Cell Proliferation/drug effects ; Dinoprostone/biosynthesis ; Glucose/pharmacology ; Glucose Tolerance Test ; Group V Phospholipases A2/genetics ; Insulin/metabolism ; Insulin Secretion ; Insulin-Secreting Cells/drug effects ; Islets of Langerhans/ultrastructure ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; RNA-Induced Silencing Complex
مستخلص: Purpose: Group X (GX) and group V (GV) secretory phospholipase A 2 (sPLA 2 ) potently release arachidonic acid (AA) from the plasma membrane of intact cells. We previously demonstrated that GX sPLA 2 negatively regulates glucose-stimulated insulin secretion (GSIS) by a prostaglandin E2 (PGE2)-dependent mechanism. In this study we investigated whether GV sPLA 2 similarly regulates GSIS.
Methods: GSIS and pancreatic islet-size were assessed in wild-type (WT) and GV sPLA 2 -knock out (GV KO) mice. GSIS was also assessed ex vivo in isolated islets and in vitro using MIN6 pancreatic beta cell lines with or without GV sPLA 2 overexpression or silencing.
Results: GSIS was significantly decreased in islets isolated from GV KO mice compared to WT mice and in MIN6 cells with siRNA-mediated GV sPLA 2 suppression. MIN6 cells overexpressing GV sPLA 2 (MIN6-GV) showed a significant increase in GSIS compared to control cells. Though the amount of AA released into the media by MIN6-GV cells was significantly higher, PGE2 production was not enhanced or cAMP content decreased compared to control MIN6 cells. Surprisingly, GV KO mice exhibited a significant increase in plasma insulin levels following i.p. injection of glucose compared to WT mice. This increase in GSIS in GV KO mice was associated with a significant increase in pancreatic islet size and number of proliferating cells in β-islets compared to WT mice.
Conclusions: Deficiency of GV sPLA 2 results in diminished GSIS in isolated pancreatic beta-cells. However, the reduced GSIS in islets lacking GV sPLA 2 appears to be compensated by increased islet mass in GV KO mice.
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معلومات مُعتمدة: P20 GM103527 United States GM NIGMS NIH HHS; R01 DK082419 United States DK NIDDK NIH HHS; P20 GM103527 National Institute of General Medical Sciences; R01 DK082419 National Heart, Lung, and Blood Institute
فهرسة مساهمة: Keywords: Arachidonic acid; GV sPLA2; Insulin secretion; Type 2 diabetes; β-islet mass
المشرفين على المادة: 0 (Insulin)
0 (RNA-Induced Silencing Complex)
27YG812J1I (Arachidonic Acid)
EC 3.1.1.4 (Group V Phospholipases A2)
IY9XDZ35W2 (Glucose)
K7Q1JQR04M (Dinoprostone)
تواريخ الأحداث: Date Created: 20170822 Date Completed: 20180801 Latest Revision: 20191008
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5693688
DOI: 10.1007/s12020-017-1379-1
PMID: 28825176
قاعدة البيانات: MEDLINE
الوصف
تدمد:1559-0100
DOI:10.1007/s12020-017-1379-1