دورية أكاديمية
Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia.
| العنوان: | Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia. |
|---|---|
| المؤلفون: | Ross PA; School of BioSciences, Bio21 Institute and University of Melbourne; paross@student.unimelb.edu.au., Axford JK; School of BioSciences, Bio21 Institute and University of Melbourne., Richardson KM; School of BioSciences, Bio21 Institute and University of Melbourne., Endersby-Harshman NM; School of BioSciences, Bio21 Institute and University of Melbourne., Hoffmann AA; School of BioSciences, Bio21 Institute and University of Melbourne. |
| المصدر: | Journal of visualized experiments : JoVE [J Vis Exp] 2017 Aug 14 (126). Date of Electronic Publication: 2017 Aug 14. |
| نوع المنشور: | Journal Article; Video-Audio Media |
| اللغة: | English |
| بيانات الدورية: | Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]- |
| مواضيع طبية MeSH: | Wolbachia*, Aedes/*microbiology, Aedes/physiology ; Animals ; Female ; Larva ; Male ; Oviposition |
| مستخلص: | Aedes aegypti mosquitoes experimentally infected with Wolbachia are being utilized in programs to control the spread of arboviruses such as dengue, chikungunya and Zika. Wolbachia-infected mosquitoes can be released into the field to either reduce population sizes through incompatible matings or to transform populations with mosquitoes that are refractory to virus transmission. For these strategies to succeed, the mosquitoes released into the field from the laboratory must be competitive with native mosquitoes. However, maintaining mosquitoes in the laboratory can result in inbreeding, genetic drift and laboratory adaptation which can reduce their fitness in the field and may confound the results of experiments. To test the suitability of different Wolbachia infections for deployment in the field, it is necessary to maintain mosquitoes in a controlled laboratory environment across multiple generations. We describe a simple protocol for maintaining Ae. aegypti mosquitoes in the laboratory, which is suitable for both Wolbachia-infected and wild-type mosquitoes. The methods minimize laboratory adaptation and implement outcrossing to increase the relevance of experiments to field mosquitoes. Additionally, colonies are maintained under optimal conditions to maximize their fitness for open field releases. |
| References: | J Med Entomol. 1988 May;25(3):178-82. (PMID: 3392713) PLoS Negl Trop Dis. 2010 Jul 13;4(7):e748. (PMID: 20644622) Nature. 2011 Aug 24;476(7361):454-7. (PMID: 21866160) J Am Mosq Control Assoc. 2000 Jun;16(2):138-42. (PMID: 10901637) PLoS Negl Trop Dis. 2009 Sep 15;3(9):e516. (PMID: 19753103) PLoS Negl Trop Dis. 2014 Dec 11;8(12):e3294. (PMID: 25502564) PLoS Pathog. 2016 Feb 18;12(2):e1005434. (PMID: 26891349) J Med Entomol. 2011 Jan;48(1):76-84. (PMID: 21337952) PLoS One. 2015 Aug 07;10(8):e0135194. (PMID: 26252474) Bull World Health Organ. 1973;48(1):49-56. (PMID: 4541148) PLoS Negl Trop Dis. 2012;6(11):e1892. (PMID: 23133693) PLoS Pathog. 2017 Jan 5;13(1):e1006006. (PMID: 28056065) Sci Rep. 2016 Sep 23;6:33846. (PMID: 27659038) Sci Rep. 2016 Jul 01;6:28792. (PMID: 27364935) Parasit Vectors. 2015 Oct 28;8:563. (PMID: 26510523) Am J Trop Med Hyg. 2016 Apr;94(4):780-5. (PMID: 26880776) J Med Entomol. 1984 Nov 29;21(6):732-40. (PMID: 6502631) Am J Trop Med Hyg. 1984 Jul;33(4):690-4. (PMID: 6476217) J Med Entomol. 2011 Jul;48(4):914-23. (PMID: 21845954) Heredity (Edinb). 2017 Jan;118(1):10-20. (PMID: 27703153) Am J Trop Med Hyg. 1977 May;26(3):553-61. (PMID: 869107) Trends Parasitol. 2003 Aug;19(8):349-55. (PMID: 12901936) J Am Mosq Control Assoc. 2008 Dec;24(4):591-3. (PMID: 19181071) Int J Environ Res Public Health. 2016 Dec 21;13(12):. (PMID: 28009851) Am J Trop Med Hyg. 2009 Mar;80(3):395-400. (PMID: 19270288) Nat Biotechnol. 2011 Oct 30;29(11):1034-7. (PMID: 22037376) Med Vet Entomol. 2000 Mar;14(1):31-7. (PMID: 10759309) Nature. 2011 Aug 24;476(7361):450-3. (PMID: 21866159) Science. 2005 Oct 14;310(5746):326-8. (PMID: 16224027) Evol Appl. 2015 Sep;8(8):751-68. (PMID: 26366194) Am J Trop Med Hyg. 2016 Mar;94(3):507-16. (PMID: 26711515) PLoS Negl Trop Dis. 2012;6(11):e1797. (PMID: 23166845) Evol Appl. 2015 Jul 15;9(1):212-30. (PMID: 27087849) Cell. 2009 Dec 24;139(7):1268-78. (PMID: 20064373) Elife. 2015 Jun 30;4:e08347. (PMID: 26126267) Acta Trop. 2017 Feb;166:155-163. (PMID: 27876643) Am J Trop Med Hyg. 2016 Nov 2;95(5):1169-1173. (PMID: 27573623) PLoS Negl Trop Dis. 2011 Apr 12;5(4):e1015. (PMID: 21532736) Annu Rev Entomol. 1989;34:401-21. (PMID: 2564759) PLoS One. 2016 Aug 01;11(8):e0160196. (PMID: 27479050) Bull Entomol Res. 2017 Aug;107(4):419-430. (PMID: 27974065) Microb Ecol. 2014 Jan;67(1):205-18. (PMID: 24337107) Am J Trop Med Hyg. 2013 Jul;89(1):78-92. (PMID: 23716403) J Med Entomol. 2000 Jan;37(1):77-88. (PMID: 15218910) PLoS Negl Trop Dis. 2016 Jan 08;10(1):e0004320. (PMID: 26745630) J Med Entomol. 2016 Jun 16;:. (PMID: 27313167) J Vector Ecol. 2015 Dec;40(2):292-300. (PMID: 26611964) Philos Trans R Soc Lond B Biol Sci. 2015 Apr 5;370(1665):null. (PMID: 25688023) Bull World Health Organ. 1967;36(4):586-9. (PMID: 5299457) J Vis Exp. 2014 Jan 04;(83):e3579. (PMID: 24430003) Genetics. 2011 Feb;187(2):583-95. (PMID: 21135075) Sci Transl Med. 2015 Mar 18;7(279):279ra37. (PMID: 25787763) Trop Parasitol. 2014 Jan;4(1):53-5. (PMID: 24754030) Appl Environ Microbiol. 2012 Jul;78(13):4740-3. (PMID: 22522691) Science. 2009 Jan 2;323(5910):141-4. (PMID: 19119237) Nat Rev Microbiol. 2013 Mar;11(3):181-93. (PMID: 23411863) |
| معلومات مُعتمدة: | United Kingdom Wellcome Trust |
| تواريخ الأحداث: | Date Created: 20170823 Date Completed: 20180129 Latest Revision: 20191008 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC5614331 |
| DOI: | 10.3791/56124 |
| PMID: | 28829414 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-087X |
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| DOI: | 10.3791/56124 |