دورية أكاديمية
أعرض في EDS

Effects of cycloheximide on the interpretation of ribosome profiling experiments in Schizosaccharomyces pombe.

التفاصيل البيبلوغرافية
العنوان: Effects of cycloheximide on the interpretation of ribosome profiling experiments in Schizosaccharomyces pombe.
المؤلفون: Duncan CDS; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, United Kingdom., Mata J; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, United Kingdom. jm593@cam.ac.uk.
المصدر: Scientific reports [Sci Rep] 2017 Sep 04; Vol. 7 (1), pp. 10331. Date of Electronic Publication: 2017 Sep 04.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Antifungal Agents/*pharmacology , Cycloheximide/*pharmacology , Protein Synthesis Inhibitors/*pharmacology , Ribosomes/*metabolism , Schizosaccharomyces/*drug effects , Schizosaccharomyces/*physiology, Codon ; Open Reading Frames ; Protein Biosynthesis/drug effects ; Saccharomyces cerevisiae/drug effects ; Saccharomyces cerevisiae/physiology
مستخلص: Stress conditions lead to global and gene-specific changes in RNA translation. Ribosome profiling experiments have identified genome-wide alterations in the distribution of ribosomes along mRNAs. However, it is contentious whether these changes reflect real responses, or whether they are artefacts caused by the use of inhibitors of translation (notably cycloheximide). To address this issue we performed ribosome profiling with the fission yeast Schizosaccharomyces pombe under conditions of exponential growth (unstressed) and nitrogen starvation (nutritional stress), and both in the presence and absence of cycloheximide. We examined several aspects of the translational response, including density of ribosomal footprints on coding sequences, 5' leader ribosomal densities, distribution of ribosomes along coding sequences, and ribosome codon occupancies. Cycloheximide had minor effects on overall ribosome density, which affected mostly mRNAs encoding ribosomal proteins. Nitrogen starvation caused an accumulation of ribosomes on 5' leaders in both cycloheximide-treated and untreated cells. By contrast, stress-induced ribosome accumulation on the 5' side of coding sequences was cycloheximide-dependent. Finally, codon occupancy showed strong positive correlations in cycloheximide-treated and untreated cells. Our results demonstrate that cycloheximide does influence some of the results of ribosome profiling experiments, although it is not clear if this effect is always artefactual.
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معلومات مُعتمدة: BB/M021483/1 United Kingdom Biotechnology and Biological Sciences Research Council
المشرفين على المادة: 0 (Antifungal Agents)
0 (Codon)
0 (Protein Synthesis Inhibitors)
98600C0908 (Cycloheximide)
تواريخ الأحداث: Date Created: 20170906 Date Completed: 20190503 Latest Revision: 20220321
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC5583251
DOI: 10.1038/s41598-017-10650-1
PMID: 28871121
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-017-10650-1