دورية أكاديمية
أعرض في EDS

Molecular characterisation of rifampicin-resistant Mycobacterium tuberculosis strains from Malawi.

التفاصيل البيبلوغرافية
العنوان: Molecular characterisation of rifampicin-resistant Mycobacterium tuberculosis strains from Malawi.
المؤلفون: Chikaonda T; Department of Molecular Medicine and Haematology, Faculty of Health Sciences, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa.; UNC Project, Lilongwe, Malawi., Ketseoglou I; Department of Molecular Medicine and Haematology, Faculty of Health Sciences, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa., Nguluwe N; UNC Project, Lilongwe, Malawi., Krysiak R; UNC Project, Lilongwe, Malawi., Thengolose I; UNC Project, Lilongwe, Malawi., Nyakwawa F; Malawi National Tuberculosis Programme, Lilongwe, Malawi., Rosenberg NE; UNC Project, Lilongwe, Malawi., Stanley C; UNC Project, Lilongwe, Malawi., Mpunga J; Malawi National Tuberculosis Programme, Lilongwe, Malawi., Hoffman IF; UNC Project, Lilongwe, Malawi.; University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States., Papathanasopoulos MA; Department of Molecular Medicine and Haematology, Faculty of Health Sciences, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa., Hosseinipour M; UNC Project, Lilongwe, Malawi.; University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States., Scott L; Department of Molecular Medicine and Haematology, Faculty of Health Sciences, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa., Stevens W; Department of Molecular Medicine and Haematology, Faculty of Health Sciences, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa.
المصدر: African journal of laboratory medicine [Afr J Lab Med] 2017 Mar 31; Vol. 6 (2), pp. 463. Date of Electronic Publication: 2017 Mar 31 (Print Publication: 2017).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: AOSIS OpenJournals Country of Publication: South Africa NLM ID: 101603205 Publication Model: eCollection Cited Medium: Print ISSN: 2225-2002 (Print) Linking ISSN: 22252002 NLM ISO Abbreviation: Afr J Lab Med Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Durbanville, South Africa : AOSIS OpenJournals, [2012]-
مستخلص: Background: Availability and access to the detection of resistance to anti-tuberculosis drugs remains a significant challenge in Malawi due to limited diagnostic services. The Xpert ® MTB/RIF can detect Mycobacterium tuberculosis and resistance to rifampicin in a single, rapid assay. Rifampicin-resistant M. tuberculosis has not been well studied in Malawi.
Objectives: We aimed to determine mutations in the rifampicin resistance determining region (RRDR) of the rpo B gene of M. tuberculosis strains which were defined as resistant to rifampicin by the Xpert MTB/RIF assay.
Methods: Rifampicin-resistant isolates from 43 adult patients (≥ 18 years) from various districts of Malawi were characterised for mutations in the RRDR (codons 507-533) of the rpo B gene by DNA sequencing.
Results: Mutations were found in 37/43 (86%) of the resistant isolates in codons 511, 512, 513, 516, 522, 526 and 531. The most common mutations were in codons 526 (38%), 531 (29.7%) and 516 (16.2%). Mutations were not found in 6/43 (14%) of the resistant isolates. No novel rpo B mutations other than those previously described were found among the rifampicin-resistant M. tuberculosis complex strains.
Conclusion: This study is the first to characterise rifampicin resistance in Malawi. The chain-termination DNA sequencing employed in this study is a standard method for the determination of nucleotide sequences and can be used to confirm rifampicin resistance obtained using other assays, including the Xpert MTB/RIF. Further molecular cluster analysis, such as spoligotyping and DNA finger printing, is still required to determine transmission dynamics and the epidemiological link of the mutated strains.
Competing Interests: The authors declare that they have no financial or personal relationships which may have inappropriately influenced them in writing this article.
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معلومات مُعتمدة: D43 TW001039 United States TW FIC NIH HHS; D43 TW010060 United States TW FIC NIH HHS; P30 AI050410 United States AI NIAID NIH HHS; UM1 AI106701 United States AI NIAID NIH HHS
تواريخ الأحداث: Date Created: 20170908 Latest Revision: 20230806
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5523914
DOI: 10.4102/ajlm.v6i2.463
PMID: 28879159
قاعدة البيانات: MEDLINE
الوصف
تدمد:2225-2002
DOI:10.4102/ajlm.v6i2.463