دورية أكاديمية
أعرض في EDS

A Salmonella typhimurium ghost vaccine induces cytokine expression in vitro and immune responses in vivo and protects rats against homologous and heterologous challenges.

التفاصيل البيبلوغرافية
العنوان: A Salmonella typhimurium ghost vaccine induces cytokine expression in vitro and immune responses in vivo and protects rats against homologous and heterologous challenges.
المؤلفون: Vinod N; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Noh HB; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Oh S; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Ji S; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Park HJ; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Lee KS; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Kim SC; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Park HO; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea.; New Drug R&D Center, Bioneer Corporation, Daejeon, Korea., Yang JS; New Drug R&D Center, Bioneer Corporation, Daejeon, Korea., Choi CW; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea.
المصدر: PloS one [PLoS One] 2017 Sep 29; Vol. 12 (9), pp. e0185488. Date of Electronic Publication: 2017 Sep 29 (Print Publication: 2017).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Bacterial Vaccines/*administration & dosage , Cytokines/*metabolism , Salmonella Infections, Animal/*prevention & control , Salmonella typhimurium/*immunology, Animals ; Antibodies, Bacterial/biosynthesis ; Antibodies, Bacterial/blood ; Bacterial Vaccines/immunology ; CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; Cell Line ; Cytokines/genetics ; Inflammation Mediators/metabolism ; Mice ; RNA, Messenger/genetics ; Rats ; Salmonella Infections, Animal/immunology
مستخلص: Salmonella enteritidis and Salmonella typhimurium are important food-borne bacterial pathogens, which are responsible for diarrhea and gastroenteritis in humans and animals. In this study, S. typhimurium bacterial ghost (STG) was generated based on minimum inhibitory concentration (MIC) of sodium hydroxide (NaOH). Experimental studies performed using in vitro and in vivo experimental model systems to characterize effects of STG as a vaccine candidate. When compared with murine macrophages (RAW 264.7) exposed to PBS buffer (98.1%), the macrophages exposed to formalin-killed inactivated cells (FKC), live wild-type bacterial cells and NaOH-induced STG at 1 × 108 CFU/mL showed 85.6%, 66.5% and 84.6% cell viability, respectively. It suggests that STG significantly reduces the cytotoxic effect of wild-type bacterial cells. Furthermore, STG is an excellent inducer for mRNAs of pro-inflammatory cytokine (TNF-α, IL-1β) and factor (iNOS), anti-inflammatory cytokine (IL-10) and dual activities (IL-6) in the stimulated macrophage cells. In vivo, STG vaccine induced humoral and cellular immune responses and protection against homologous and heterologous challenges in rats. Furthermore, the immunogenicity and protective efficacy of STG vaccine were compared with those of FKC and non-vaccinated PBS control groups. The vaccinated rats from STG group exhibited higher levels of serum IgG antibody responses, serum bactericidal antibodies, and CD4+ and CD8+ T-cell populations than those of the FKC and PBS control groups. Most importantly, after challenge with homologous and heterologous strains, the bacterial loads in the STG group were markedly lower than the FKC and PBS control groups. In conclusion, these findings suggest that the STG vaccine induces protective immunity against homologous and heterologous challenges.
References: Microb Pathog. 1991 Feb;10(2):149-58. (PMID: 1890952)
Microb Biotechnol. 2012 Mar;5(2):283-94. (PMID: 22103353)
Med Mal Infect. 1996 Apr;26 Suppl 3:420-5. (PMID: 17292313)
J Appl Microbiol. 2001 Sep;91(3):442-52. (PMID: 11556909)
Nat Immunol. 2001 Apr;2(4):361-7. (PMID: 11276208)
Poult Sci. 1994 Jul;73(7):1077-81. (PMID: 7937468)
Clin Vaccine Immunol. 2013 Jun;20(6):795-802. (PMID: 23536691)
Clin Infect Dis. 2010 Mar 15;50(6):882-9. (PMID: 20158401)
Vaccine. 1992;10(4):247-54. (PMID: 1561832)
Infect Immun. 1995 May;63(5):2004-11. (PMID: 7729915)
Microbes Infect. 2001 Mar;3(3):237-47. (PMID: 11358718)
APMIS. 1989 Sep;97(9):825-32. (PMID: 2675938)
ScientificWorldJournal. 2013;2013:545741. (PMID: 23576904)
Proc Natl Acad Sci U S A. 1989 Jul;86(13):5054-8. (PMID: 2544889)
Saudi Pharm J. 2014 Jul;22(3):273-9. (PMID: 25061413)
Am J Vet Res. 1992 Oct;53(10):1895-9. (PMID: 1456538)
Bioeng Bugs. 2010 Sep-Oct;1(5):326-36. (PMID: 21326832)
Infect Immun. 1994 Dec;62(12):5519-27. (PMID: 7960134)
Curr Mol Med. 2002 Jun;2(4):393-406. (PMID: 12108950)
J Gen Microbiol. 1980 Jul;119(1):123-31. (PMID: 6157776)
J Biomed Biotechnol. 2012;2012:760730. (PMID: 22719209)
Infect Immun. 1970 Nov;2(5):549-55. (PMID: 16557876)
Microb Pathog. 2001 Aug;31(2):91-102. (PMID: 11453704)
Immunobiology. 2011 May;216(5):558-65. (PMID: 21247655)
J Biotechnol. 2000 Sep 29;83(1-2):115-23. (PMID: 11000467)
J Clin Microbiol. 1994 Jan;32(1):199-201. (PMID: 8126179)
Infect Immun. 2015 Jul;83(7):2957-65. (PMID: 25964469)
J Biol Chem. 1981 Jul 25;256(14):7305-10. (PMID: 6166612)
J Immunol. 1976 Jan;116(1):8-11. (PMID: 1107425)
Vet Res. 2012 May 23;43:44. (PMID: 22620989)
Vaccine. 1998 Jan;16(1):45-54. (PMID: 9607008)
Infect Immun. 2012 Apr;80(4):1502-9. (PMID: 22290147)
Vaccine. 1996 Mar;14(4):251-9. (PMID: 8744548)
Epidemiol Infect. 1990 Jun;104(3):413-26. (PMID: 2189743)
Vaccine. 2014 May 30;32(26):3249-55. (PMID: 24721534)
J Immunol. 2000 Jun 1;164(11):5894-904. (PMID: 10820271)
Am J Vet Res. 1985 Jul;46(7):1415-21. (PMID: 3896069)
Med Mal Infect. 1996 Apr;26 Suppl 3:426-30. (PMID: 17292314)
Eur J Biochem. 1980;107(1):137-43. (PMID: 6995111)
Int J Mol Sci. 2016 Nov 15;17 (11):. (PMID: 27854308)
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2561-5. (PMID: 11226278)
Vaccine. 2009 Mar 4;27(10):1571-8. (PMID: 19166892)
J Exp Med. 1997 Aug 18;186(4):569-80. (PMID: 9254655)
Trends Microbiol. 2001 Jan;9(1):29-33. (PMID: 11166240)
J Bacteriol. 1978 Oct;136(1):286-94. (PMID: 361696)
J Leukoc Biol. 2000 Apr;67(4):457-63. (PMID: 10770276)
Microbes Infect. 2001 Nov-Dec;3(14-15):1239-48. (PMID: 11755412)
Med Mal Infect. 1996 Apr;26 Suppl 3:415-9. (PMID: 17292312)
Clin Infect Dis. 2001 Jan 15;32(2):263-9. (PMID: 11170916)
Am J Vet Res. 1993 Aug;54(8):1249-55. (PMID: 8214891)
J Immunol. 2002 Aug 15;169(4):2196-203. (PMID: 12165550)
J Exp Med. 2000 Jul 17;192(2):237-48. (PMID: 10899910)
Eur J Immunol. 2006 Nov;36(11):2939-50. (PMID: 17048271)
Immunology. 1997 Apr;90(4):618-25. (PMID: 9176117)
Adv Exp Med Biol. 2009;655:159-70. (PMID: 20047041)
المشرفين على المادة: 0 (Antibodies, Bacterial)
0 (Bacterial Vaccines)
0 (Cytokines)
0 (Inflammation Mediators)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20170930 Date Completed: 20171103 Latest Revision: 20181113
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC5621678
DOI: 10.1371/journal.pone.0185488
PMID: 28961267
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0185488