دورية أكاديمية
A Salmonella typhimurium ghost vaccine induces cytokine expression in vitro and immune responses in vivo and protects rats against homologous and heterologous challenges.
العنوان: | A Salmonella typhimurium ghost vaccine induces cytokine expression in vitro and immune responses in vivo and protects rats against homologous and heterologous challenges. |
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المؤلفون: | Vinod N; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Noh HB; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Oh S; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Ji S; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Park HJ; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Lee KS; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Kim SC; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea., Park HO; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea.; New Drug R&D Center, Bioneer Corporation, Daejeon, Korea., Yang JS; New Drug R&D Center, Bioneer Corporation, Daejeon, Korea., Choi CW; Department of Biology & Medicinal Science, Pai Chai University, Daejeon, Korea. |
المصدر: | PloS one [PLoS One] 2017 Sep 29; Vol. 12 (9), pp. e0185488. Date of Electronic Publication: 2017 Sep 29 (Print Publication: 2017). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science |
مواضيع طبية MeSH: | Bacterial Vaccines/*administration & dosage , Cytokines/*metabolism , Salmonella Infections, Animal/*prevention & control , Salmonella typhimurium/*immunology, Animals ; Antibodies, Bacterial/biosynthesis ; Antibodies, Bacterial/blood ; Bacterial Vaccines/immunology ; CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; Cell Line ; Cytokines/genetics ; Inflammation Mediators/metabolism ; Mice ; RNA, Messenger/genetics ; Rats ; Salmonella Infections, Animal/immunology |
مستخلص: | Salmonella enteritidis and Salmonella typhimurium are important food-borne bacterial pathogens, which are responsible for diarrhea and gastroenteritis in humans and animals. In this study, S. typhimurium bacterial ghost (STG) was generated based on minimum inhibitory concentration (MIC) of sodium hydroxide (NaOH). Experimental studies performed using in vitro and in vivo experimental model systems to characterize effects of STG as a vaccine candidate. When compared with murine macrophages (RAW 264.7) exposed to PBS buffer (98.1%), the macrophages exposed to formalin-killed inactivated cells (FKC), live wild-type bacterial cells and NaOH-induced STG at 1 × 108 CFU/mL showed 85.6%, 66.5% and 84.6% cell viability, respectively. It suggests that STG significantly reduces the cytotoxic effect of wild-type bacterial cells. Furthermore, STG is an excellent inducer for mRNAs of pro-inflammatory cytokine (TNF-α, IL-1β) and factor (iNOS), anti-inflammatory cytokine (IL-10) and dual activities (IL-6) in the stimulated macrophage cells. In vivo, STG vaccine induced humoral and cellular immune responses and protection against homologous and heterologous challenges in rats. Furthermore, the immunogenicity and protective efficacy of STG vaccine were compared with those of FKC and non-vaccinated PBS control groups. The vaccinated rats from STG group exhibited higher levels of serum IgG antibody responses, serum bactericidal antibodies, and CD4+ and CD8+ T-cell populations than those of the FKC and PBS control groups. Most importantly, after challenge with homologous and heterologous strains, the bacterial loads in the STG group were markedly lower than the FKC and PBS control groups. In conclusion, these findings suggest that the STG vaccine induces protective immunity against homologous and heterologous challenges. |
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المشرفين على المادة: | 0 (Antibodies, Bacterial) 0 (Bacterial Vaccines) 0 (Cytokines) 0 (Inflammation Mediators) 0 (RNA, Messenger) |
تواريخ الأحداث: | Date Created: 20170930 Date Completed: 20171103 Latest Revision: 20181113 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC5621678 |
DOI: | 10.1371/journal.pone.0185488 |
PMID: | 28961267 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1932-6203 |
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DOI: | 10.1371/journal.pone.0185488 |