دورية أكاديمية
Aluminium-induced cell death requires upregulation of NtVPE1 gene coding vacuolar processing enzyme in tobacco (Nicotiana tabacum L.).
| العنوان: | Aluminium-induced cell death requires upregulation of NtVPE1 gene coding vacuolar processing enzyme in tobacco (Nicotiana tabacum L.). |
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| المؤلفون: | Kariya K; Institute of Plant Science and Resources, Okayama University, Chuo-2-20-1, Kurashiki 710-0046, Japan. Electronic address: ppd34jtt@okayama-u.ac.jp., Tsuchiya Y; Institute of Plant Science and Resources, Okayama University, Chuo-2-20-1, Kurashiki 710-0046, Japan. Electronic address: y-tsuchi@okayama-u.ac.jp., Sasaki T; Institute of Plant Science and Resources, Okayama University, Chuo-2-20-1, Kurashiki 710-0046, Japan. Electronic address: tsasaki@okayama-u.ac.jp., Yamamoto Y; Institute of Plant Science and Resources, Okayama University, Chuo-2-20-1, Kurashiki 710-0046, Japan. Electronic address: yoko@rib.okayama-u.ac.jp. |
| المصدر: | Journal of inorganic biochemistry [J Inorg Biochem] 2018 Apr; Vol. 181, pp. 152-161. Date of Electronic Publication: 2017 Sep 12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 7905788 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3344 (Electronic) Linking ISSN: 01620134 NLM ISO Abbreviation: J Inorg Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Elsevier. |
| مواضيع طبية MeSH: | Aluminum/*toxicity , Cysteine Endopeptidases/*metabolism , Plant Proteins/*metabolism , Plant Root Cap/*drug effects , Soil Pollutants/*toxicity , Nicotiana/*drug effects, Adsorption ; Aluminum/chemistry ; Aluminum/metabolism ; Biomarkers/metabolism ; Cell Line ; Cell Membrane/chemistry ; Cell Membrane/drug effects ; Cell Membrane/metabolism ; Cell Shape/drug effects ; Cell Survival/drug effects ; Cysteine Endopeptidases/chemistry ; Cysteine Endopeptidases/genetics ; Enzyme Induction/drug effects ; Isoenzymes/antagonists & inhibitors ; Isoenzymes/chemistry ; Isoenzymes/genetics ; Isoenzymes/metabolism ; Kinetics ; Meristem/cytology ; Meristem/drug effects ; Meristem/growth & development ; Meristem/metabolism ; Plant Proteins/agonists ; Plant Proteins/antagonists & inhibitors ; Plant Proteins/genetics ; Plant Root Cap/cytology ; Plant Root Cap/growth & development ; Plant Root Cap/metabolism ; Plant Roots/cytology ; Plant Roots/drug effects ; Plant Roots/growth & development ; Plant Roots/metabolism ; RNA Interference ; Soil Pollutants/chemistry ; Soil Pollutants/metabolism ; Surface Properties ; Nicotiana/cytology ; Nicotiana/growth & development ; Nicotiana/metabolism |
| مستخلص: | Cell death mechanism triggered by aluminium (Al) ion was investigated at root apex of tobacco (cultivar Bright Yellow) and in cultured tobacco cell line BY-2 derived from Bright Yellow, focusing on VPE genes (NtVPE1a, NtVPE1b, NtVPE2, NtVPE3). Cell death was detected as a loss of integrity of the plasma membrane by vital staining with fluorescein diacetate (in root apex) and Evans blue (in BY-2), respectively. At root apex, the upregulation of gene expression of VPE1a and VPE1b was observed significantly after 9h of Al exposure in parallel with an enhancement of cell death, while the upregulation of VPE2 and VPE3 were observed later. Similarly, in BY-2 cells, the upregulation of VPE1a and VPE1b and the enhancement of cell death were synchronously observed after 3-h exposure to Al, while the upregulation of VPE2 and VPE3 occurred later. RNA interference (RNAi) lines of each of the VPEs were constructed in BY-2 cells. Comparative studies between wild-type and the RNAi lines indicated that both Al-enhanced VPE activity and Al-induced cell death were significantly suppressed in the RNAi lines of VPE1 (dual suppressor of VPE1a and VPE1b), but not in the RNAi lines of VPE2 and that of VPE3. Taken together, we conclude that the upregulation of VPE1 gene expression and following enhancement of VPE activity under Al stress cause cell death in actively growing or elongating cells of tobacco. (Copyright © 2017 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aluminium toxicity; BY-2 tobacco cell line; Cell death; NtVPE1; Root elongation inhibition; Vacuolar processing enzyme |
| المشرفين على المادة: | 0 (Biomarkers) 0 (Isoenzymes) 0 (Plant Proteins) 0 (Soil Pollutants) CPD4NFA903 (Aluminum) EC 3.4.22.- (Cysteine Endopeptidases) EC 3.4.22.- (vacuolar processing enzyme) |
| تواريخ الأحداث: | Date Created: 20171003 Date Completed: 20181029 Latest Revision: 20231213 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jinorgbio.2017.09.008 |
| PMID: | 28967473 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3344 |
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| DOI: | 10.1016/j.jinorgbio.2017.09.008 |