دورية أكاديمية
MiR-146a inhibits proliferation and induces apoptosis in murine osteoblastic MC3T3-E1 by regulating Bcl2.
| العنوان: | MiR-146a inhibits proliferation and induces apoptosis in murine osteoblastic MC3T3-E1 by regulating Bcl2. |
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| المؤلفون: | Zhang B; Department of Orthopedics, Shuyang Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Suqian, China. zhangbinsy@126.com., Yi J, Zhang CL, Zhang QH, Xu JF, Shen HQ, Ge DW |
| المصدر: | European review for medical and pharmacological sciences [Eur Rev Med Pharmacol Sci] 2017 Oct; Vol. 21 (17), pp. 3754-3762. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Verduci Country of Publication: Italy NLM ID: 9717360 Publication Model: Print Cited Medium: Internet ISSN: 2284-0729 (Electronic) Linking ISSN: 11283602 NLM ISO Abbreviation: Eur Rev Med Pharmacol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Rome : Verduci, [1997- |
| مواضيع طبية MeSH: | Apoptosis/*genetics , MicroRNAs/*genetics , Osteoblasts/*metabolism , Proto-Oncogene Proteins c-bcl-2/*genetics, Animals ; Apoptosis/drug effects ; Cell Proliferation/drug effects ; Cell Proliferation/genetics ; Cells, Cultured ; Dexamethasone/pharmacology ; Mice ; Osteoblasts/drug effects ; Proto-Oncogene Proteins c-bcl-2/biosynthesis |
| مستخلص: | Objective: The dysregulation of proliferation and apoptosis plays a significant role in the pathogenesis of hormone-induced osteonecrosis of femoral head (ONFH). The research aimed to explore the regulatory role of miR-146a in dexamethasone (DEX)-induced proliferation and apoptosis change in MC3T3-E1 cells from murine osteoblastic. Material and Methods: In this study, MC3T3-E1 was co-cultured with 10-7 DEX for 6 h, then RT-PCR was employed to test the expression level of miR-146a and Bcl2. CCK8 assay and flow cytometry were adopted to verify miR-146a could regulate proliferation and apoptosis. After transfected MC3T3-E1 with mimics and inhibitor, RT-PCR and Western blot was used to detect Bcl2 expression level. Results: In DEX treated MC3T3-E1 cells showed higher MiR-146a expression level and lower Bcl2 expression level. MiR-146a could inhibit proliferation and promotes apoptosis in murine osteoblastic MC3T3-E1 cells. Additionally, Bcl2 gene is regulated by MiR-146a. Conclusions: The MiR-146a expression level increased, while Bcl2 has low expression level in dexamethasone treated MC3T3-E1 cells. MiR-146a regulates proliferation and apoptosis of mouse bone cells. The low expression level of Bcl2 in DEX treated MC3T3-E1 cells is caused by increased MiR-146a level. |
| المشرفين على المادة: | 0 (BCL2 protein, human) 0 (MicroRNAs) 0 (Mirn146 microRNA, mouse) 0 (Proto-Oncogene Proteins c-bcl-2) 7S5I7G3JQL (Dexamethasone) |
| تواريخ الأحداث: | Date Created: 20171005 Date Completed: 20180716 Latest Revision: 20181202 |
| رمز التحديث: | 20221213 |
| PMID: | 28975995 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2284-0729 |
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