دورية أكاديمية
A novel mycovirus associated to Alternaria alternata comprises a distinct lineage in Partitiviridae.
| العنوان: | A novel mycovirus associated to Alternaria alternata comprises a distinct lineage in Partitiviridae. |
|---|---|
| المؤلفون: | Xavier ADS; Departamento de Fitopatologia, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, MG 36570-900, Brazil., Barros APO; Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, MG 36570-900, Brazil., Godinho MT; Departamento de Fitopatologia, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, MG 36570-900, Brazil., Zerbini FM; Departamento de Fitopatologia, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, MG 36570-900, Brazil; National Research Institute on Plant-Pest Interactions, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil., Souza FO; Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, MG 36570-900, Brazil., Bruckner FP; Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, MG 36570-900, Brazil., Alfenas-Zerbini P; Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, MG 36570-900, Brazil; National Research Institute on Plant-Pest Interactions, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil. Electronic address: palfenas@ufv.br. |
| المصدر: | Virus research [Virus Res] 2018 Jan 15; Vol. 244, pp. 21-26. Date of Electronic Publication: 2017 Oct 10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8410979 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7492 (Electronic) Linking ISSN: 01681702 NLM ISO Abbreviation: Virus Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier Science, c1984- |
| مواضيع طبية MeSH: | Genome, Viral* , Phylogeny*, Alternaria/*virology , Fungal Viruses/*genetics , RNA Viruses/*genetics , RNA, Viral/*genetics, Base Sequence ; Capsid Proteins/genetics ; Fungal Viruses/classification ; Genotype ; Microbial Interactions ; Phenotype ; RNA Viruses/classification ; RNA, Double-Stranded/genetics ; RNA-Dependent RNA Polymerase/genetics ; Sequence Alignment ; Sequence Homology, Nucleic Acid |
| مستخلص: | In the present work, we report a novel mycovirus that infects Alternaria alternata. The mycovirus has isometric particles of approximately 30nm and the genome consists of two molecules of dsRNA, dsRNA1 with 1833bp, encoding a putative RNA-dependent RNA polymerase (RdRp) and dsRNA2, with 1680bp in length, encoding the putative capsid protein (CP). RdRp analysis revealed low amino acid identity with RdRps with species in the genus Gammapartitivirus, and the alignment of the RdRp revealed all the six conserved motifs present in members of Partitiviridae. The putative coat protein (CP) analysis revealed similarity with the putative CP of Botryosphaeria dothidea partitivirus 1 (BdPV1), a divergent partitivirus. We propose that Alternaria alternata partitivirus 1 (AtPV1) is a novel species and comprises a distinct lineage related to genus Gammapartitivirus in the family Partitiviridae, apparently on the threshold of radiation of a new genus, together with BdPV1. Vertical transmission tests showed that AtPV1 was transmitted to 100% conidial progeny and standard curing was unable to eliminate it from the host, characterizing it as a persistent virus. The absence of a virus-free isogenic lineage prevented us from accessing the details of the interaction between AtPV1 and A. alternata. Therefore, it remains unclear whether the morphological plasticity observed or the inability of the A. alternata isolate AVi1 to cause disease in plants is associated with AtPV1 infection. (Copyright © 2017 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Divergent viruses; Fungal phenotypic plasticity |
| المشرفين على المادة: | 0 (Capsid Proteins) 0 (RNA, Double-Stranded) 0 (RNA, Viral) EC 2.7.7.48 (RNA-Dependent RNA Polymerase) |
| تواريخ الأحداث: | Date Created: 20171013 Date Completed: 20180912 Latest Revision: 20201209 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.virusres.2017.10.007 |
| PMID: | 29024687 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7492 |
|---|---|
| DOI: | 10.1016/j.virusres.2017.10.007 |