دورية أكاديمية
Neutralizing activity and safety of human monoclonal antibodies against hepatitis C virus.
| العنوان: | Neutralizing activity and safety of human monoclonal antibodies against hepatitis C virus. |
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| المؤلفون: | Abdel Malak CA; Department of Chemistry, Faculty of Science, Damietta University, Damietta, Egypt., Abelhafez TH; Department of Microbial Biotechnology, National Research Center, Giza, Egypt., Tabll AA; Department of Microbial Biotechnology, National Research Center, Giza, Egypt., Mashaly MM; Department of Chemistry, Faculty of Science, Damietta University, Damietta, Egypt., El Shenawy R; Department of Microbial Biotechnology, National Research Center, Giza, Egypt., El-Abd YS; Department of Microbial Biotechnology, National Research Center, Giza, Egypt., Shaker MH; Animal Health Research Institute, Giza, Egypt., El-Awady MK; Department of Microbial Biotechnology, National Research Center, Giza, Egypt. |
| المصدر: | Human antibodies [Hum Antibodies] 2017; Vol. 26 (3), pp. 127-134. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: IOS Press Country of Publication: Netherlands NLM ID: 9711270 Publication Model: Print Cited Medium: Internet ISSN: 1875-869X (Electronic) Linking ISSN: 10932607 NLM ISO Abbreviation: Hum Antibodies Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : IOS Press Original Publication: Wilton, CT : Forefront Pub., c1997- |
| مواضيع طبية MeSH: | Antibodies, Monoclonal/*immunology , Antibodies, Neutralizing/*immunology , Hepacivirus/*immunology , Hepatitis C Antibodies/*immunology, Animals ; Cell Line ; Hepatitis C/immunology ; Humans ; Mice ; Neutralization Tests/methods ; Viral Envelope Proteins/immunology |
| مستخلص: | Aim: Assessment of the neutralizing activity of human monoclonal antibodies against HCV and also study their safety in experimental small animals (Swiss mice). Materials and Methods: Assessment of neutralizing activity of human monoclonal antibodies against HCV envelope regions (E1, E2) by two methods: by HCV cc infectious system 1) and by using positive HCV positive serum as source of HCV particles genotype 4a (neutralizing assay 2). Dot ELISA was used to study the activity of the generated antibodies. Safety and toxicity of the generated human antibodies were tested by assessing the changes in the biochemistry of liver function and kidney function tests, Complete blood counts (CBC) and studying the pathological changes with different concentrations of purified human antibodies were carried out.. Results: Human Abs # 5 & 11 showed neutralizing activity by (neutralizing assay 2) but were not neutralizing by HCV cc assay. Human Abs # 12 & 15 showed neutralizing activity by the two methods i.e our generated human antibodies Abs# 5 &11 & 12 & 15 were neutralizing for HCV genotype 4a and Abs # 12 & 15 were neutralizing for HCV genotypes 4a and 2a. Liver and kidney functions and CBC results indicated that doses of 10 μg, 100 μg were safe. The histopathological results indicated that the dose of 10 μg of purified human monoclonal antibodies per mouse body weight was safe. Conclusion: The generated human monoclonal antibodies can be used to develop potent immunotherapy agents that can be administrated for the post-transplantation patients to prevent the recurrence of HCV infection. Also, the monoclonal antibodies can be used to develop a candidate vaccine against HCV. |
| فهرسة مساهمة: | Keywords: HCV; liver transplantation; monoclonal antibodies; vaccine development |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Antibodies, Neutralizing) 0 (Hepatitis C Antibodies) 0 (Viral Envelope Proteins) |
| تواريخ الأحداث: | Date Created: 20171018 Date Completed: 20180924 Latest Revision: 20180924 |
| رمز التحديث: | 20240628 |
| DOI: | 10.3233/HAB-170330 |
| PMID: | 29036810 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1875-869X |
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| DOI: | 10.3233/HAB-170330 |