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Photobiomodulation of local alterations induced by BthTX-I, a phospholipase A 2 myotoxin from Bothrops jararacussu snake venom: In vivo and in vitro evaluation.

التفاصيل البيبلوغرافية
العنوان: Photobiomodulation of local alterations induced by BthTX-I, a phospholipase A 2 myotoxin from Bothrops jararacussu snake venom: In vivo and in vitro evaluation.
المؤلفون: Santos ASD; Department of Medicine, Universidade Nove de Julho (UNINOVE), Rua Vergueiro, 235, 01504-000, São Paulo, SP, Brazil., Guimarães-Sousa L; Institute of Research and Development, University of Vale do Paraíba (UNIVAP), Avenida Shishima Hifumi, 2911, 12244-000, Urbanova, São José dos Campos, SP, Brazil., Costa MS; Institute of Research and Development, University of Vale do Paraíba (UNIVAP), Avenida Shishima Hifumi, 2911, 12244-000, Urbanova, São José dos Campos, SP, Brazil., Zamuner LF; Department of Medicine, Universidade Nove de Julho (UNINOVE), Rua Vergueiro, 235, 01504-000, São Paulo, SP, Brazil., Sousa NC; Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP),Rua Tessália Vieira de Camargo, 126, Cidade Universitária Zeferino Vaz, 13083-887, Campinas, SP, Brazil., Hyslop S; Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP),Rua Tessália Vieira de Camargo, 126, Cidade Universitária Zeferino Vaz, 13083-887, Campinas, SP, Brazil., Soares AM; Fundação Oswaldo Cruz, FIOCRUZ, Rua da Beira, 7671, 76812-245, Porto Velho, RO, Brazil., Chavantes MC; Department of Medicine, Universidade Nove de Julho (UNINOVE), Rua Vergueiro, 235, 01504-000, São Paulo, SP, Brazil., Cogo JC; Programa de Pós-Graduação em Bioengenharia e Engenharia Biomédica, Universidade Brasil, Rua Carolina Fonseca 235/584, 08230-030, São Paulo, SP, Brazil., Zamuner SR; Department of Medicine, Universidade Nove de Julho (UNINOVE), Rua Vergueiro, 235, 01504-000, São Paulo, SP, Brazil. Electronic address: stella.rz@uni9.pro.br.
المصدر: International journal of biological macromolecules [Int J Biol Macromol] 2018 Feb; Vol. 107 (Pt B), pp. 2020-2025. Date of Electronic Publication: 2017 Oct 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
مواضيع طبية MeSH: Low-Level Light Therapy*, Bothrops/*metabolism , Phospholipases A2/*pharmacology , Snake Venoms/*pharmacology, Animals ; Cell Line ; Cell Survival/radiation effects ; Dinoprostone/metabolism ; Edema/pathology ; Leukocytes/pathology ; Male ; Mice ; Muscle Cells/radiation effects ; Peritoneum/pathology
مستخلص: This report describes the effect of photobiomodulation (PBM) on edema formation, leukocyte influx, prostaglandin E 2 (PGE 2 ) biosynthesis and cytotoxicity caused by bothropstoxin-I (BthTX-I), a phospholipase A 2 (PLA 2 ) homologue isolated from Bothrops jararacussu snake venom. Swiss mice or C2C12 cells were irradiated with low-level laser (LLL) at 685nm wavelength, an energy density of 4.6J/cm 2 and an irradiation time of 13s. To evaluate the effect on edema formation and leukocyte influx, LLL was applied to the site of inoculation 30min and 3h post-injection. C2C12 cells were exposed to BthTX-I and immediately irradiated. PBM significantly reduced paw edema formation, peritoneal leukocyte influx and PGE 2 synthesis, but increased the viability of C2C12 muscle cells after BthTX-I incubation. These findings demonstrate that PBM attenuated the inflammatory events induced by BthTX-I. The attenuation of PGE 2 synthesis could be an important factor in the reduced inflammatory response caused by laser irradiation. The ability of LLL irradiation to protect muscle cells against the deleterious effects of BthTX-I may indicate preservation of the plasma membrane.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: BthTX-I; Cytotoxicity; Inflammation; Photobiomodulation
المشرفين على المادة: 0 (Snake Venoms)
EC 3.1.1.4 (Phospholipases A2)
K7Q1JQR04M (Dinoprostone)
تواريخ الأحداث: Date Created: 20171018 Date Completed: 20180727 Latest Revision: 20180727
رمز التحديث: 20240628
DOI: 10.1016/j.ijbiomac.2017.10.076
PMID: 29037876
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0003
DOI:10.1016/j.ijbiomac.2017.10.076