دورية أكاديمية
Autonomous control of metabolic state by a quorum sensing (QS)-mediated regulator for bisabolene production in engineered E. coli.
| العنوان: | Autonomous control of metabolic state by a quorum sensing (QS)-mediated regulator for bisabolene production in engineered E. coli. |
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| المؤلفون: | Kim EM; Joint BioEnergy Institute, 5885 Hollis St., Emeryville, CA 94608, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Woo HM; Joint BioEnergy Institute, 5885 Hollis St., Emeryville, CA 94608, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Tian T; Joint BioEnergy Institute, 5885 Hollis St., Emeryville, CA 94608, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Yilmaz S; Joint BioEnergy Institute, 5885 Hollis St., Emeryville, CA 94608, USA; Department of Bioengineering and Biotechnology, Sandia National Laboratory, Livermore, CA, USA., Javidpour P; Joint BioEnergy Institute, 5885 Hollis St., Emeryville, CA 94608, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Keasling JD; Joint BioEnergy Institute, 5885 Hollis St., Emeryville, CA 94608, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA; Department of Bioengineering, University of California, Berkeley, CA, USA; Department of Chemical & Biomolecular Engineering, University of California, Berkeley, CA, USA; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Alle, DK2970 Hørsholm, Denmark., Lee TS; Joint BioEnergy Institute, 5885 Hollis St., Emeryville, CA 94608, USA; Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. Electronic address: tslee@lbl.gov. |
| المصدر: | Metabolic engineering [Metab Eng] 2017 Nov; Vol. 44, pp. 325-336. Date of Electronic Publication: 2017 Nov 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: Belgium NLM ID: 9815657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-7184 (Electronic) Linking ISSN: 10967176 NLM ISO Abbreviation: Metab Eng Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Brugge, Belgium ; Orlando, FL : Academic Press, c1999- |
| مواضيع طبية MeSH: | Escherichia coli*/genetics , Escherichia coli*/metabolism , Metabolic Engineering* , Quorum Sensing* , Repressor Proteins*/genetics , Repressor Proteins*/metabolism , Trans-Activators*/genetics , Trans-Activators*/metabolism, Aliivibrio fischeri/*genetics , Sesquiterpenes/*metabolism |
| مستخلص: | Inducible gene expression systems are widely used in microbial host strains for protein and commodity chemical production because of their extensive characterization and ease of use. However, some of these systems have disadvantages such as leaky expression, lack of dynamic control, and the prohibitively high costs of inducers associated with large-scale production. Quorum sensing (QS) systems in bacteria control gene expression in response to population density, and the LuxI/R system from Vibrio fischeri is a well-studied example. A QS system could be ideal for biofuel production strains as it is self-regulated and does not require the addition of inducer compounds, which reduce operational costs for inducer. In this study, a QS system was developed for inducer-free production of the biofuel compound bisabolene from engineered E. coli. Seven variants of the Sensor plasmid, which carry the luxI-luxR genes, and four variants of the Response plasmid, which carry bisabolene producing pathway genes under the control of the P (Copyright © 2017 International Metabolic Engineering Society. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Biofuel; Bisabolene; LuxI/R; Quorum sensing (QS); Synthetic biology |
| المشرفين على المادة: | 0 (Repressor Proteins) 0 (Sesquiterpenes) 0 (Trans-Activators) 115038-68-1 (LuxR autoinducer binding proteins) |
| تواريخ الأحداث: | Date Created: 20171114 Date Completed: 20180710 Latest Revision: 20220408 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ymben.2017.11.004 |
| PMID: | 29129823 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-7184 |
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| DOI: | 10.1016/j.ymben.2017.11.004 |