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Flavonoidal Constituents, Antioxidant, Antimicrobial, and Cytotoxic Activities of Dipterygium glaucum Grown in Kingdom of Saudi Arabia.

التفاصيل البيبلوغرافية
العنوان: Flavonoidal Constituents, Antioxidant, Antimicrobial, and Cytotoxic Activities of Dipterygium glaucum Grown in Kingdom of Saudi Arabia.
المؤلفون: Shaheen U; Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah Al Mukarramah 21955, Saudi Arabia.; Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt., Shoeib NA; Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah Al Mukarramah 21955, Saudi Arabia.; Department of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta 31527, Egypt., Temraz A; Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah Al Mukarramah 21955, Saudi Arabia.; Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt., Abdelhady MIS; Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah Al Mukarramah 21955, Saudi Arabia.; Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
المصدر: Pharmacognosy magazine [Pharmacogn Mag] 2017 Oct; Vol. 13 (Suppl 3), pp. S484-S488. Date of Electronic Publication: 2017 Oct 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: SAGE Publications Country of Publication: United States NLM ID: 101300403 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0973-1296 (Print) Linking ISSN: 09731296 NLM ISO Abbreviation: Pharmacogn Mag Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2023- : [Thousand Oaks] : SAGE Publications
Original Publication: 2005-<2009> : Bangalore : Phcog.Net, Al-Ameen College of Pharmacy
مستخلص: Background: Dipterygium glaucum Decne. herb is one of the common traditional plants with multiple medicinal uses.
Objective: To isolate the major constituents and to investigate the antioxidant, antimicrobial, and cytotoxic activities of this herb.
Materials and Methods: Methanolic extract of D. glaucum herb was fractionated using n -hexane, dichloromethane, and n -butanol. Butanol fraction was chromatographed using column chromatography and preparative thin layer chromatography to isolate the major constituents. Isolated compounds were elucidated by means of spectroscopic methods, including 1D, 2D NMR ( 1 H, 13 C, DEPT, COSY, HSQC, HMBC, NEOSY) and MS analysis. Total phenolic content using Folin-Ciocalteu reagent and antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay of the total methanolic extract were evaluated. Cytotoxic potential of both methanolic extract and butanol fraction was tested using a crystal violet viability assay. Antimicrobial activities of both extracts were investigated using diffusion agar technique.
Results: Apigenin 6, 8-di-C-glucopyranoside (vicenin-2), quercetin-3`- O -methyl-3- O -glucopyranoside, quercetin-3`- O -methyl-3- O -galactopyranoside, quercetin-3- O - β -D-glucopyranoside, and quercetin-3- O - β -D-galactopyranoside were isolated and elucidated. Total phenolic content was (83.89 mg gallic acid equivalent/g extract). The EC 50 value of scavenging DPPH radical was 152.0 ± 2 mg/mL. Butanol fraction showed the highest cytotoxic activity against cervical and breast carcinoma cells (IC 50 3.6 and 6.1 mg/mL, respectively). Both methanolic extract and butanol fraction showed wide spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria and some fungi. The highest activity was from methanolic extract against Enterococcus faecalis (83.25%) and against Candida tropicalis (77.03%) as compared to reference antibiotics.
Conclusion: Data obtained from this study demonstrate that D. glaucum possesses significant antioxidant, cytotoxic, and antimicrobial activities which could be ascribed to its flavonoidal content.
Summary: Dipterygium glaucum Decne. herb is one of the common traditional plants with multiple medicinal uses in KSAFive flvonidal glycosides were isolated and elucidatedThis study demonstrated that D. glaucum possesses significant antioxidant, cytotoxic, and antimicrobial activities. Abbreviations used: KSA: Kingdom of Saudi Arabia; TLC: Thin Layer Chromatography; DPPH: 2,2`-diphenyl-1-picrylhydrazyl; EC50: Half maximal effective concentration; IC 50 : Half maximal inhibitory concentration; DMSO: dimethyl sulfoxide; NMR: Nuclear Magnetic Resonance; ESIMS: Electrospray ionization mass spectrometry; MeOH: Methyl alcohol.
Competing Interests: There are no conflicts of interest.
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فهرسة مساهمة: Keywords: Antimicrobial; Dipterygium glaucum; antioxidant; cytotoxic; flavonoids
تواريخ الأحداث: Date Created: 20171117 Latest Revision: 20220310
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5669086
DOI: 10.4103/pm.pm_44_16
PMID: 29142403
قاعدة البيانات: MEDLINE
الوصف
تدمد:0973-1296
DOI:10.4103/pm.pm_44_16