دورية أكاديمية
Sagittal Plane Kinematic Gait Analysis in C57BL/6 Mice Subjected to MOG35-55 Induced Experimental Autoimmune Encephalomyelitis.
| العنوان: | Sagittal Plane Kinematic Gait Analysis in C57BL/6 Mice Subjected to MOG35-55 Induced Experimental Autoimmune Encephalomyelitis. |
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| المؤلفون: | Fiander MD; Pharmacology, Dalhousie University., Chedrawe MA; Pharmacology, Dalhousie University., Lamport AC; Pharmacology, Dalhousie University., Akay T; Medical Neuroscience, Dalhousie University; Turgay.Akay@Dal.Ca., Robertson GS; Pharmacology, Dalhousie University; Psychiatry, Dalhousie University; robertgs@dal.ca. |
| المصدر: | Journal of visualized experiments : JoVE [J Vis Exp] 2017 Nov 04 (129). Date of Electronic Publication: 2017 Nov 04. |
| نوع المنشور: | Journal Article; Video-Audio Media |
| اللغة: | English |
| بيانات الدورية: | Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]- |
| مواضيع طبية MeSH: | Encephalomyelitis, Autoimmune, Experimental/*chemically induced , Gait/*physiology , Walking/*physiology, Animals ; Disease Models, Animal ; Encephalomyelitis, Autoimmune, Experimental/complications ; Humans ; Male ; Mice ; Mice, Inbred C57BL |
| مستخلص: | Kinematic gait analysis in the sagittal plane has frequently been used to characterize motor deficits in multiple sclerosis (MS). We describe the application of these techniques to identify gait deficits in a mouse model of MS, known as experimental autoimmune encephalomyelitis (EAE). Paralysis and motor deficits in mice subjected to EAE are typically assessed using a clinical scoring scale. However, this scale yields only ordinal data that provides little information about the precise nature of the motor deficits. EAE disease severity has also been assessed by rotarod performance, which provides a measure of general motor coordination. By contrast, kinematic gait analysis of the hind limb in the sagittal plane generates highly precise information about how movement is impaired. To perform this procedure, reflective markers are placed on a hind limb to detect joint movement while a mouse is walking on a treadmill. Motion analysis software is used to measure movement of the markers during walking. Kinematic gait parameters are then derived from the resultant data. We show how these gait parameters can be used to quantify impaired movements of the hip, knee, and ankle joints in EAE. These techniques may be used to better understand disease mechanisms and identify potential treatments for MS and other neurodegenerative disorders that impair mobility. |
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| تواريخ الأحداث: | Date Created: 20171121 Date Completed: 20180329 Latest Revision: 20191104 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC5755292 |
| DOI: | 10.3791/56032 |
| PMID: | 29155752 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-087X |
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| DOI: | 10.3791/56032 |