دورية أكاديمية
أعرض في EDS

Comparison of the effect of the aerobic glycolysis inhibitor dichloroacetate and of the Krebs cycle inhibitor LW6 on cellular and humoral alloimmunity.

التفاصيل البيبلوغرافية
العنوان: Comparison of the effect of the aerobic glycolysis inhibitor dichloroacetate and of the Krebs cycle inhibitor LW6 on cellular and humoral alloimmunity.
المؤلفون: Eleftheriadis T; Department of Nephrology, Faculty of Medicine, University of Thessaly, 41110 Larissa, Greece., Pissas G; Department of Nephrology, Faculty of Medicine, University of Thessaly, 41110 Larissa, Greece., Mavropoulos A; Department of Rheumatology and Clinical Immunology, Faculty of Medicine, University of Thessaly, 41110 Larissa, Greece., Liakopoulos V; Department of Nephrology, Faculty of Medicine, University of Thessaly, 41110 Larissa, Greece., Stefanidis I; Department of Nephrology, Faculty of Medicine, University of Thessaly, 41110 Larissa, Greece.
المصدر: Biomedical reports [Biomed Rep] 2017 Nov; Vol. 7 (5), pp. 439-444. Date of Electronic Publication: 2017 Sep 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: [Spandidos Publications] Country of Publication: England NLM ID: 101613227 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2049-9434 (Print) Linking ISSN: 20499434 NLM ISO Abbreviation: Biomed Rep Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [London] : [Spandidos Publications], [2013]-
مستخلص: Cell metabolism is altered during T-cell and B-cell activation and differentiation. Clarifying the exact metabolic shifts may lead to the development of new immunosuppressive medications. In this study, the effect of the aerobic glycolysis inhibitor dichloroacetate (DCA) and of the Krebs cycle enzyme malate dehydrogenase 2 (MDH2) inhibitor LW6 on T-cell alloimmune clonal expansion and on alloantibody production, was evaluated. T-cell clonal expansion was assessed in two-way mixed lymphocyte reaction (MLR). Humoral alloimmunity was evaluated by the alloantibody production in one-way MLR. For this purpose, an antibody-mediated complement-dependent cytotoxicity assay was developed in which the supernatants from one-way MLRs were used against resting peripheral blood mononuclear cells (PBMCs) derived from the same individual that contributed the stimulator cells for the respective MLR. DCA had a minimum effect on alloimmune T-cell clonal expansion, whereas it increased humoral immunity significantly. LW6 decreased both alloimmune T-cell proliferation and alloantibody production. The results indicate that MDH2 may be a perfect target for the development of new immunosuppressive medications, especially when inhibition of both cellular and humoral alloimmunity is desirable.
References: Immunity. 2011 Dec 23;35(6):871-82. (PMID: 22195744)
Int J Immunopathol Pharmacol. 2017 Mar;30(1):44-57. (PMID: 27903843)
Comp Biochem Physiol A Comp Physiol. 1988;91(4):765-8. (PMID: 2907443)
Metabolism. 1989 Nov;38(11):1124-44. (PMID: 2554095)
Clin Vaccine Immunol. 2008 Jan;15(1):88-94. (PMID: 18032598)
Angew Chem Int Ed Engl. 2013 Sep 23;52(39):10286-9. (PMID: 23934700)
J Biol Chem. 2014 Mar 7;289(10):7011-24. (PMID: 24469453)
Cell Metab. 2012 Jan 4;15(1):110-21. (PMID: 22225880)
Immunity. 2004 Jul;21(1):81-93. (PMID: 15345222)
Exp Cell Res. 1973 Mar 15;77(1):127-35. (PMID: 4690164)
Metabolism. 1971 Jan;20(1):2-12. (PMID: 5539063)
Am J Kidney Dis. 2015 Jun;65(6):956-66. (PMID: 25911314)
Clin Exp Immunol. 1999 Feb;115(2):301-8. (PMID: 9933457)
Immunity. 2016 Jul 19;45(1):60-73. (PMID: 27396958)
Cell. 2013 Jun 6;153(6):1239-51. (PMID: 23746840)
Arthritis Res Ther. 2009;11(5):R132. (PMID: 19723321)
Semin Immunol. 2012 Dec;24(6):405-13. (PMID: 23290889)
Exp Ther Med. 2015 Nov;10(5):1959-1966. (PMID: 26640580)
J Proteome Res. 2011 Sep 2;10(9):4165-76. (PMID: 21744784)
J Immunol Methods. 2009 Jul 31;346(1-2):75-9. (PMID: 19465022)
Magn Reson Med. 1993 Mar;29(3):317-26. (PMID: 8450740)
J Immunol. 2016 Jan 1;196 (1):80-90. (PMID: 26608911)
J Immunol. 1982 Feb;128(2):696-701. (PMID: 6459383)
Curr Mol Med. 2013 May;13(4):514-34. (PMID: 22934847)
N Engl J Med. 1999 Dec 2;341(23):1725-30. (PMID: 10580071)
Int J Cancer. 2013 Sep 1;133(5):1107-18. (PMID: 23420584)
J Immunol. 2011 Mar 15;186(6):3299-303. (PMID: 21317389)
J Immunotoxicol. 2007 Apr;4(2):107-15. (PMID: 18958719)
Am J Physiol Lung Cell Mol Physiol. 2012 Feb 1;302(3):L300-7. (PMID: 22080752)
J Cell Physiol. 1985 Aug;124(2):275-82. (PMID: 4044655)
Int Urol Nephrol. 2017 Sep;49(9):1691-1697. (PMID: 28508247)
Immunity. 2013 Feb 21;38(2):225-36. (PMID: 23415911)
Mol Med Rep. 2016 Apr;13(4):3370-6. (PMID: 26935268)
J Immunol. 2014 Apr 15;192(8):3626-36. (PMID: 24616478)
Immunol Lett. 2017 Aug;188:108-115. (PMID: 28687234)
فهرسة مساهمة: Keywords: B-cells; Krebs cycle; LW6; T-cells; aerobic glycolysis; alloimmunity; dichloroacetate; transplantation
تواريخ الأحداث: Date Created: 20171129 Latest Revision: 20201001
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC5700391
DOI: 10.3892/br.2017.980
PMID: 29181155
قاعدة البيانات: MEDLINE
الوصف
تدمد:2049-9434
DOI:10.3892/br.2017.980