دورية أكاديمية
Use of photodynamic inactivation for in vitro reduction of prevalent bacteria in Fournier's Gangrene.
| العنوان: | Use of photodynamic inactivation for in vitro reduction of prevalent bacteria in Fournier's Gangrene. |
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| المؤلفون: | Pereira NM; Departamento de Engenharia Biomédica, Universidade Brasil, São Paulo, SP, Brasil., Feitosa LS; Departamento de Engenharia Biomédica, Universidade Brasil, São Paulo, SP, Brasil., Navarro RS; Departamento de Engenharia Biomédica, Universidade Brasil, São Paulo, SP, Brasil., Kozusny-Andreani DI; Departamento de Engenharia Biomédica, Universidade Brasil, São Paulo, SP, Brasil., Carvalho NMP; Departamento de Engenharia Biomédica, Universidade Brasil, São Paulo, SP, Brasil. |
| المصدر: | International braz j urol : official journal of the Brazilian Society of Urology [Int Braz J Urol] 2018 Jan-Feb; Vol. 44 (1), pp. 150-155. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Brazilian Society of Urology Country of Publication: Brazil NLM ID: 101158091 Publication Model: Print Cited Medium: Internet ISSN: 1677-6119 (Electronic) Linking ISSN: 16775538 NLM ISO Abbreviation: Int Braz J Urol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Rio de Janerio, RJ, Brazil : Brazilian Society of Urology, [2002]- |
| مواضيع طبية MeSH: | Photochemotherapy*, Fournier Gangrene/*microbiology , Fournier Gangrene/*therapy , Methylene Blue/*therapeutic use , Photosensitizing Agents/*therapeutic use, Clostridium perfringens/isolation & purification ; Escherichia coli/isolation & purification ; In Vitro Techniques ; Staphylococcus aureus/isolation & purification |
| مستخلص: | Fournier's Gangrene (FG) is an infectious disease caused by several synergic microbes, with high morbidity and mortality rates; therefore, the search for new less invasive and mutilating treatments, with faster recovery, has been proposed. Surgical intervention, the use of several systemic and topic antibiotics, and hyperbaric oxygen therapy are currently the best approach for the treatment of these patients. The use of Photodynamic Inactivation (PDI) aims to lower morbidity and mortality, by reducing bacterial microbiota and speeding wound healing. In the present study, viable bacteria were separated in four groups: Group L-/F- (no irradiation with red laser and absence of methylene blue photosensitizer), Group L-/F+ (no irradiation with red laser and presence of methylene blue), Group L+/F- (irradiation with red laser and absence of methylene blue) and L+/F+ (irradiation with red laser associated to methylene blue). In all groups, exposure time to treatment was 5, 10 and 15 minutes. The concentration of methylene blue photosensitizer was 0.1mg/L, and the dose of red laser (660nm wave length) was 176.9mW/cm2. Following irradiation, the reduction of number of bacteria was evaluated, and the results were expressed in colony forming units (CFU) and as exponential reduction. As the main results, in the L+/F+ group, there were no Clostridium perfringens and Staphylococcus aureus CFUs and there was a reduction of Escherichia coli that was not observed in the other groups. Competing Interests: Conflict of interest: None declared. (Copyright® by the International Brazilian Journal of Urology.) |
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| فهرسة مساهمة: | Keywords: Clostridium perfringens; Fournier Gangrene; Methylene Blue |
| المشرفين على المادة: | 0 (Photosensitizing Agents) T42P99266K (Methylene Blue) |
| تواريخ الأحداث: | Date Created: 20171209 Date Completed: 20180601 Latest Revision: 20181113 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC5815545 |
| DOI: | 10.1590/S1677-5538.IBJU.2017.0312 |
| PMID: | 29219279 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1677-6119 |
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| DOI: | 10.1590/S1677-5538.IBJU.2017.0312 |