دورية أكاديمية
EphA7 regulates claudin6 and pronephros development in Xenopus.
| العنوان: | EphA7 regulates claudin6 and pronephros development in Xenopus. |
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| المؤلفون: | Sun J; State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China., Wang X; State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China., Shi Y; State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China; Department of Clinical Laboratory, Children's Hospital of Chongqing Medical University, Chongqing, China., Li J; State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China., Li C; State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China., Shi Z; Shenzhen Key Laboratory of Cell Microenvironment, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, China., Chen Y; Shenzhen Key Laboratory of Cell Microenvironment, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, China., Mao B; State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China. Electronic address: mao@mail.kiz.ac.cn. |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2018 Jan 08; Vol. 495 (2), pp. 1580-1587. Date of Electronic Publication: 2017 Dec 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Claudins/*metabolism , Pronephros/*embryology , Receptor, EphA7/*metabolism , Xenopus Proteins/*metabolism , Xenopus laevis/*embryology , Xenopus laevis/*metabolism, Animals ; Cell Membrane/metabolism ; Gene Expression Regulation, Developmental ; Gene Knockdown Techniques ; Oligodeoxyribonucleotides, Antisense/genetics ; Pronephros/metabolism ; Receptor, EphA7/antagonists & inhibitors ; Receptor, EphA7/genetics ; Solubility ; Xenopus Proteins/antagonists & inhibitors ; Xenopus Proteins/genetics ; Xenopus laevis/genetics |
| مستخلص: | Eph/ephrin molecules are widely expressed during embryonic development, and function in a variety of developmental processes. Here we studied the roles of the Eph receptor EphA7 and its soluble form in Xenopus pronephros development. EphA7 is specifically expressed in pronephric tubules at tadpole stages and knockdown of EphA7 by a translation blocking morpholino led to defects in tubule cell differentiation and morphogenesis. A soluble form of EphA7 (sEphA7) was also identified. Interestingly, the membrane level of claudin6 (CLDN6), a tetraspan transmembrane tight junction protein, was dramatically reduced in the translation blocking morpholino injected embryos, but not when a splicing morpholino was used, which blocks only the full length EphA7. In cultured cells, EphA7 binds and phosphorylates CLDN6, and reduces its distribution at the cell surface. Our work suggests a role of EphA7 in the regulation of cell adhesion during pronephros development, whereas sEphA7 works as an antagonist. (Copyright © 2017 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Claudin6; EphA7; Morphogenesis; Pronephros development; Soluble EphA7; Xenopus |
| المشرفين على المادة: | 0 (Claudins) 0 (Oligodeoxyribonucleotides, Antisense) 0 (Xenopus Proteins) EC 2.7.10.1 (Receptor, EphA7) MRC5FX426I (claudin 6) |
| تواريخ الأحداث: | Date Created: 20171211 Date Completed: 20180220 Latest Revision: 20181211 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.bbrc.2017.12.027 |
| PMID: | 29223398 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
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| DOI: | 10.1016/j.bbrc.2017.12.027 |