دورية أكاديمية
All-trans retinoic acid induces mitochondria-mediated apoptosis of human adipose-derived stem cells and affects the balance of the adipogenic differentiation.
| العنوان: | All-trans retinoic acid induces mitochondria-mediated apoptosis of human adipose-derived stem cells and affects the balance of the adipogenic differentiation. |
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| المؤلفون: | Schweich LC; Stem Cell, Cell Therapy and Toxicological Genetics Research Centre (CeTroGen), 'Maria Aparecida Pedrossian' University Hospital, Brazilian Hospital Services Company (EBSERH), Campo Grande, Mato Grosso do Sul, Brazil; Federal University of Mato Grosso do Sul (UFMS), Graduate Programme in Health and Development in the Central-West Region, School of Medicine (FAMED), Campo Grande, Mato Grosso do Sul, Brazil., Oliveira EJT; Stem Cell, Cell Therapy and Toxicological Genetics Research Centre (CeTroGen), 'Maria Aparecida Pedrossian' University Hospital, Brazilian Hospital Services Company (EBSERH), Campo Grande, Mato Grosso do Sul, Brazil; State University of Londrina (UEL), Graduate Programme in Genetics and Molecular Biology, Department of General Biology, Londrina, Paraná, Brazil., Pesarini JR; Stem Cell, Cell Therapy and Toxicological Genetics Research Centre (CeTroGen), 'Maria Aparecida Pedrossian' University Hospital, Brazilian Hospital Services Company (EBSERH), Campo Grande, Mato Grosso do Sul, Brazil; Federal University of Mato Grosso do Sul (UFMS), Graduate Programme in Health and Development in the Central-West Region, School of Medicine (FAMED), Campo Grande, Mato Grosso do Sul, Brazil., Hermeto LC; Stem Cell, Cell Therapy and Toxicological Genetics Research Centre (CeTroGen), 'Maria Aparecida Pedrossian' University Hospital, Brazilian Hospital Services Company (EBSERH), Campo Grande, Mato Grosso do Sul, Brazil; Federal University of Mato Grosso do Sul (UFMS), Graduate Programme in Veterinary Science, School of Veterinary Medicine and Zootechny, Campo Grande, Mato Grosso do Sul, Brazil., Camassola M; Lutheran University of Brazil (ULBRA), Laboratory of Stem Cell and Tissue Engineering, Canoas, Rio Grande do Sul, Brazil., Nardi NB; Lutheran University of Brazil (ULBRA), Laboratory of Stem Cell and Tissue Engineering, Canoas, Rio Grande do Sul, Brazil., Brochado TMM; Brazilian Institute of Therapies and Education (IBRATE), Graduate Programme in Dermatofunctional Physiotherapy, Campo Grande, Mato Grosso do Sul, Brazil., Antoniolli-Silva ACMB; Coordinator of CeTroGen, 'Maria Aparecida Pedrossian' University Hospital, Brazilian Hospital Services Company (EBSERH), Campo Grande, Mato Grosso do Sul, Brazil; Associate Professor of Federal University of Mato Grosso do Sul (UFMS) in Graduate Programme in Health and Development in the Central-West Region and School of Medicine (FAMED), Campo Grande, Mato Grosso do Sul, Brazil., Oliveira RJ; Stem Cell, Cell Therapy and Toxicological Genetics Research Centre (CeTroGen), 'Maria Aparecida Pedrossian' University Hospital, Brazilian Hospital Services Company (EBSERH), Campo Grande, Mato Grosso do Sul, Brazil; Federal University of Mato Grosso do Sul (UFMS), Graduate Programme in Health and Development in the Central-West Region, School of Medicine (FAMED), Campo Grande, Mato Grosso do Sul, Brazil; State University of Londrina (UEL), Graduate Programme in Genetics and Molecular Biology, Department of General Biology, Londrina, Paraná, Brazil. Electronic address: rodrigo.oliveira@ufms.br. |
| المصدر: | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2017 Dec; Vol. 96, pp. 1267-1274. Date of Electronic Publication: 2017 Nov 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 8213295 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1950-6007 (Electronic) Linking ISSN: 07533322 NLM ISO Abbreviation: Biomed Pharmacother Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Paris : Editions Scientifiques Elsevier Original Publication: New York, N.Y. : Masson Pub. USA, Inc., c1982- |
| مواضيع طبية MeSH: | Adipocytes/*drug effects , Apoptosis/*drug effects , Cell Differentiation/*drug effects , Mitochondria/*drug effects , Stem Cells/*drug effects , Tretinoin/*pharmacology, Adipocytes/metabolism ; Cell Cycle/drug effects ; Cell Line ; Cell Survival/drug effects ; Humans ; Mitochondria/metabolism ; Signal Transduction/drug effects ; Stem Cells/metabolism |
| مستخلص: | The all-trans-retinoic acid (ATRA) is the most active form of vitamin A that helps to regulate the proliferation, differentiation and apoptosis of several types of cells, mainly the adipocytes, and causes weight loss through the reduction of adipogenesis and lipogenesis. In this present study we demonstrated that ATRA concentrations of 20.75, 50 and 100 μM decreased the cell viability in vitro of human adipose-derived stem cells (ADSCs), and in ADSCs during adipogenic differentiation. The cells cycle assessment showed that ATRA increased the cell frequency in Sub-G1 at 4.02x and decreased it in G1 in 2.54x. Moreover, the membrane integrity loss increased by 4.66x and apoptosis increased by 33.56x in ATRA-treated cultures. The gene expression assay suggested that the treatment using ATRA leads to mitochondrial membrane permeabilization and to consequent release of proapoptotic BAK and BAX molecules (increased expression 5.5 and 5.4x respectively); in addition, it increased CASP3 expression (by 8.8x). These events may activate the Bcl-2 (4.1x increase), GADD45 (increase 3.14x) and PPAR-γ (16x increase) expressions, as well as, to reduce the p53 (by -1.38x) expression; therefore, these events should be further mediated by increased RARα expression (by 3.8x). The results evidenced that ATRA may be a good proposal for mesotherapy strategies in order to control the development of subcutaneous adipose tissue; as this tissue have a higher development in some specific areas and ATRA interferes not only in the ADSCs differentiation but also in the apoptosis of ADSCs, preadipocytes and adipocytes. (Copyright © 2017 Elsevier Masson SAS. All rights reserved.) |
| فهرسة مساهمة: | Keywords: ATRA; Adipogenic differentiation; Adipose-derived stem cells; All-trans-retinoic acid; Apoptosis; Subcutaneous adipose tissue |
| المشرفين على المادة: | 5688UTC01R (Tretinoin) |
| تواريخ الأحداث: | Date Created: 20171215 Date Completed: 20180726 Latest Revision: 20180726 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.biopha.2017.11.087 |
| PMID: | 29239820 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1950-6007 |
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| DOI: | 10.1016/j.biopha.2017.11.087 |