دورية أكاديمية
Plasmodium yoelii S4/CelTOS is important for sporozoite gliding motility and cell traversal.
| العنوان: | Plasmodium yoelii S4/CelTOS is important for sporozoite gliding motility and cell traversal. |
|---|---|
| المؤلفون: | Steel RWJ; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Pei Y; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Camargo N; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Kaushansky A; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA.; Department of Global Health, University of Washington, Seattle, Washington, USA., Dankwa DA; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Martinson T; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Nguyen T; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Betz W; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Cardamone H; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Vigdorovich V; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Dambrauskas N; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Carbonetti S; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Vaughan AM; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Sather DN; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA., Kappe SHI; Center for Infectious Disease Research, formerly Seattle Biomedical Research Institute, Seattle, Washington, USA.; Department of Global Health, University of Washington, Seattle, Washington, USA. |
| المصدر: | Cellular microbiology [Cell Microbiol] 2018 Apr; Vol. 20 (4). Date of Electronic Publication: 2018 Jan 12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Hindawi Country of Publication: India NLM ID: 100883691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1462-5822 (Electronic) Linking ISSN: 14625814 NLM ISO Abbreviation: Cell Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : Mumbai : Hindawi Original Publication: Oxford : Wiley-Blackwell, c1999- |
| مواضيع طبية MeSH: | Plasmodium yoelii/*physiology , Protozoan Proteins/*metabolism , Sporozoites/*metabolism, Animals ; Cell Movement ; Host-Parasite Interactions ; Malaria/parasitology ; Mosquito Vectors ; Plasmodium yoelii/genetics ; Protozoan Proteins/genetics |
| مستخلص: | Gliding motility and cell traversal by the Plasmodium ookinete and sporozoite invasive stages allow penetration of cellular barriers to establish infection of the mosquito vector and mammalian host, respectively. Motility and traversal are not observed in red cell infectious merozoites, and we have previously classified genes that are expressed in sporozoites but not merozoites (S genes) in order to identify proteins involved in these processes. The S4 gene has been described as criticaly involved in Cell Traversal for Ookinetes and Sporozoites (CelTOS), yet knockout parasites (s4/celtos¯) do not generate robust salivary gland sporozoite numbers, precluding a thorough analysis of S4/CelTOS function during host infection. We show here that a failure of oocysts to develop or survive in the midgut contributes to the poor mosquito infection by Plasmodium yoelii (Py) s4/celtos¯ rodent malaria parasites. We rescued this phenotype by expressing S4/CelTOS under the ookinete-specific circumsporozoite protein and thrombospondin-related anonymous protein-related protein (CTRP) promoter (S4/CelTOS CTRP ), generating robust numbers of salivary gland sporozoites lacking S4/CelTOS that were suitable for phenotypic analysis. Py S4/CelTOS CTRP sporozoites showed reduced infectivity in BALB/c mice when compared to wild-type sporozoites, although they appeared more infectious than sporozoites deficient in the related traversal protein PLP1/SPECT2 (Py plp1/spect2¯). Using in vitro assays, we substantiate the role of S4/CelTOS in sporozoite cell traversal, but also uncover a previously unappreciated role for this protein for sporozoite gliding motility. (© 2017 John Wiley & Sons Ltd.) |
| معلومات مُعتمدة: | R01 AI117234 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: cell traversal; gliding motility; host-parasite interaction; plasmodium; pre-erythrocytic infection; promoter swap |
| المشرفين على المادة: | 0 (Protozoan Proteins) |
| تواريخ الأحداث: | Date Created: 20171219 Date Completed: 20190128 Latest Revision: 20210922 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/cmi.12817 |
| PMID: | 29253313 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1462-5822 |
|---|---|
| DOI: | 10.1111/cmi.12817 |