دورية أكاديمية
أعرض في EDS

Structure of a Wbl protein and implications for NO sensing by M. tuberculosis.

التفاصيل البيبلوغرافية
العنوان: Structure of a Wbl protein and implications for NO sensing by M. tuberculosis.
المؤلفون: Kudhair BK; Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, UK.; Department of Laboratory Investigations, Faculty of Science, University of Kufa, Najaf, 54001, Iraq., Hounslow AM; Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, UK., Rolfe MD; Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, UK., Crack JC; Centre for Molecular and Structural Biochemistry, School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK., Hunt DM; Division of Mycobacterial Research, MRC National Institute for Medical Research, London, NW7 7UH, UK., Buxton RS; Division of Mycobacterial Research, MRC National Institute for Medical Research, London, NW7 7UH, UK., Smith LJ; Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, UK.; School of Pharmacy, De Montfort University, Leicester, LE1 9BH, UK., Le Brun NE; Centre for Molecular and Structural Biochemistry, School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK., Williamson MP; Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, UK., Green J; Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, UK. jeff.green@sheffield.ac.uk.
المصدر: Nature communications [Nat Commun] 2017 Dec 22; Vol. 8 (1), pp. 2280. Date of Electronic Publication: 2017 Dec 22.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Bacterial Proteins/*metabolism , DNA/*metabolism , Iron-Sulfur Proteins/*metabolism , Mycobacterium tuberculosis/*metabolism , Nitric Oxide/*metabolism , Transcription Factors/*metabolism, Bacterial Proteins/chemistry ; Gene Expression Regulation, Bacterial ; Iron-Sulfur Proteins/chemistry ; Magnetic Resonance Spectroscopy ; Mycobacterium tuberculosis/chemistry ; Protein Conformation, alpha-Helical ; Protein Structure, Tertiary ; Sigma Factor/metabolism ; Transcription Factors/chemistry ; Type VII Secretion Systems/genetics
مستخلص: Mycobacterium tuberculosis causes pulmonary tuberculosis (TB) and claims ~1.8 million human lives per annum. Host nitric oxide (NO) is important in controlling TB infection. M. tuberculosis WhiB1 is a NO-responsive Wbl protein (actinobacterial iron-sulfur proteins first identified in the 1970s). Until now, the structure of a Wbl protein has not been available. Here a NMR structural model of WhiB1 reveals that Wbl proteins are four-helix bundles with a core of three α-helices held together by a [4Fe-4S] cluster. The iron-sulfur cluster is required for formation of a complex with the major sigma factor (σ A ) and reaction with NO disassembles this complex. The WhiB1 structure suggests that loss of the iron-sulfur cluster (by nitrosylation) permits positively charged residues in the C-terminal helix to engage in DNA binding, triggering a major reprogramming of gene expression that includes components of the virulence-critical ESX-1 secretion system.
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معلومات مُعتمدة: MC_U117585867 United Kingdom MRC_ Medical Research Council
المشرفين على المادة: 0 (Bacterial Proteins)
0 (Iron-Sulfur Proteins)
0 (Sigma Factor)
0 (Transcription Factors)
0 (Type VII Secretion Systems)
0 (WhiB1 protein, Mycobacterium tuberculosis)
31C4KY9ESH (Nitric Oxide)
9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20171224 Date Completed: 20181023 Latest Revision: 20220129
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC5741622
DOI: 10.1038/s41467-017-02418-y
PMID: 29273788
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-017-02418-y