دورية أكاديمية
Monophosphoryl Lipid A-Adjuvanted Virosomes with Ni-Chelating Lipids for Attachment of Conserved Viral Proteins as Cross-Protective Influenza Vaccine.
| العنوان: | Monophosphoryl Lipid A-Adjuvanted Virosomes with Ni-Chelating Lipids for Attachment of Conserved Viral Proteins as Cross-Protective Influenza Vaccine. |
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| المؤلفون: | Dong W; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, PO Box 30001, HPC EB88, NL-9700 RB, Groningen, The Netherlands.; Division of Immunology, International Institute of Infection and Immunity, Shantou University Medical College, Shantou, 51504, Guangdong, People's Republic of China., Bhide Y; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, PO Box 30001, HPC EB88, NL-9700 RB, Groningen, The Netherlands., Marsman S; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, PO Box 30001, HPC EB88, NL-9700 RB, Groningen, The Netherlands., Holtrop M; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, PO Box 30001, HPC EB88, NL-9700 RB, Groningen, The Netherlands., Meijerhof T; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, PO Box 30001, HPC EB88, NL-9700 RB, Groningen, The Netherlands., de Vries-Idema J; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, PO Box 30001, HPC EB88, NL-9700 RB, Groningen, The Netherlands., de Haan A; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, PO Box 30001, HPC EB88, NL-9700 RB, Groningen, The Netherlands., Huckriede A; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, PO Box 30001, HPC EB88, NL-9700 RB, Groningen, The Netherlands. |
| المصدر: | Biotechnology journal [Biotechnol J] 2018 Apr; Vol. 13 (4), pp. e1700645. Date of Electronic Publication: 2018 Jan 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Verlag Country of Publication: Germany NLM ID: 101265833 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1860-7314 (Electronic) Linking ISSN: 18606768 NLM ISO Abbreviation: Biotechnol J Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Weinheim : Wiley-VCH Verlag, c2006- |
| مواضيع طبية MeSH: | Adjuvants, Immunologic/*chemistry , Lipid A/*analogs & derivatives , RNA-Binding Proteins/*immunology , Viral Core Proteins/*immunology , Virosomes/*immunology, Animals ; CD8-Positive T-Lymphocytes/metabolism ; Influenza Vaccines/immunology ; Lipid A/chemistry ; Mice ; Nickel/chemistry ; Nucleocapsid Proteins ; RAW 264.7 Cells ; T-Lymphocytes, Cytotoxic/metabolism ; Virosomes/chemistry |
| مستخلص: | Induction of CD8 + cytotoxic T cells (CTLs) to conserved internal influenza antigens, such as nucleoprotein (NP), is a promising strategy for the development of cross-protective influenza vaccines. However, influenza NP protein alone cannot induce CTL immunity due to its low capacity to activate antigen-presenting cells (APCs) and get access to the MHC class I antigen processing pathway. To facilitate the generation of NP-specific CTL immunity the authors develop a novel influenza vaccine consisting of virosomes with the Toll-like receptor 4 (TLR4) ligand monophosphoryl lipid A (MPLA) and the metal-ion-chelating lipid DOGS-NTA-Ni incorporated in the membrane. In vitro, virosomes with incorporated MPLA induce stronger activation of APCs than unadjuvanted virosomes. Virosomes modified with DOGS-NTA-Ni show high conjugation efficacy for his-tagged proteins and facilitate efficient uptake of conjugated proteins by APCs. Immunization of mice with MPLA-adjuvanted virosomes with attached NP results in priming of NP-specific CTLs while MPLA-adjuvanted virosomes with admixed NP are inefficient in priming CTLs. Both vaccines induce equally high titers of NP-specific antibodies. When challenged with heterosubtypic influenza virus, mice immunized with virosomes with attached or admixed NP are protected from severe weight loss. Yet, unexpectedly, they show more weight loss and more severe disease symptoms than mice immunized with MPLA-virosomes without NP. Taken together, these results indicate that virosomes with conjugated antigen and adjuvant incorporated in the membrane are effective in priming of CTLs and eliciting antigen-specific antibody responses in vivo. However, for protection from influenza infection NP-specific immunity appears not to be advantageous. (© 2018 The Authors. Biotechnology Journal Published by Wiley-VCHVerlag GmbH & Co. KGaA.) |
| فهرسة مساهمة: | Keywords: CD8+ T cells; MPLA; NP; Virosomes; cross-protection |
| المشرفين على المادة: | 0 (Adjuvants, Immunologic) 0 (Influenza Vaccines) 0 (Lipid A) 0 (NP protein, Influenza A virus) 0 (Nucleocapsid Proteins) 0 (RNA-Binding Proteins) 0 (Viral Core Proteins) 0 (Virosomes) 7OV03QG267 (Nickel) MWC0ET1L2P (monophosphoryl lipid A) |
| تواريخ الأحداث: | Date Created: 20171227 Date Completed: 20180910 Latest Revision: 20201209 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/biot.201700645 |
| PMID: | 29278302 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1860-7314 |
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| DOI: | 10.1002/biot.201700645 |