دورية أكاديمية
أعرض في EDS

A Peptide Nucleic Acid against MicroRNA miR-145-5p Enhances the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells.

التفاصيل البيبلوغرافية
العنوان: A Peptide Nucleic Acid against MicroRNA miR-145-5p Enhances the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells.
المؤلفون: Fabbri E; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. enrica.fabbri@unife.it., Tamanini A; Laboratory of Molecular Pathology, University-Hospital, 37126 Verona, Italy. anna.tamanini@aovr.veneto.it., Jakova T; Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, Italy. tiziana.jakova@studenti.unipr.it., Gasparello J; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. gspjsc@unife.it., Manicardi A; Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, Italy. Alex.manicardi@ugent.be., Corradini R; Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, Italy. roberto.corradini@unipr.it., Sabbioni G; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. giuseppe.sabbioni@student.unife.it., Finotti A; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. alessia.finotti@unife.it., Borgatti M; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. monica.borgatti@unife.it., Lampronti I; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. ilaria.lampronti@unife.it., Munari S; Laboratory of Molecular Pathology, University-Hospital, 37126 Verona, Italy. silvia.munari88@gmail.com., Dechecchi MC; Laboratory of Molecular Pathology, University-Hospital, 37126 Verona, Italy. cristina.dechecchi@ospedaleuniverona.it., Cabrini G; Laboratory of Molecular Pathology, University-Hospital, 37126 Verona, Italy. giulio.cabrini@univr.it., Gambari R; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. gam@unife.it.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2017 Dec 29; Vol. 23 (1). Date of Electronic Publication: 2017 Dec 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Cystic Fibrosis Transmembrane Conductance Regulator/*metabolism , Gene Expression Regulation/*drug effects , MicroRNAs/*metabolism , Peptide Nucleic Acids/*pharmacology, 3' Untranslated Regions/genetics ; Apoptosis/drug effects ; Base Sequence ; Binding Sites ; Cell Line ; Cell Proliferation/drug effects ; Cystic Fibrosis Transmembrane Conductance Regulator/genetics ; Evolution, Molecular ; Humans ; MicroRNAs/genetics ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Up-Regulation/drug effects
مستخلص: Peptide nucleic acids (PNAs) are very useful tools for gene regulation at different levels, but in particular in the last years their use for targeting microRNA (anti-miR PNAs) has provided impressive advancements. In this respect, microRNAs related to the repression of cystic fibrosis transmembrane conductance regulator (CFTR) gene, which is defective in cystic fibrosis, are of great importance in the development of new type of treatments. In this paper we propose the use of an anti-miR PNA for targeting miR-145, a microRNA reported to suppress CFTR expression. Octaarginine-anti-miR PNA conjugates were delivered to Calu-3 cells, exerting sequence dependent targeting of miR-145-5p. This allowed to enhance expression of the miR-145 regulated CFTR gene, analyzed at mRNA (RT-qPCR, Reverse Transcription quantitative Polymerase Chain Reaction) and CFTR protein (Western blotting) level.
Competing Interests: The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.
References: Am J Respir Cell Mol Biol. 2013 Oct;49(4):544-51. (PMID: 23646886)
Int J Cancer. 2018 Jan 15;142(2):308-321. (PMID: 28921929)
Oncotarget. 2017 May 16;8(20):33064-33077. (PMID: 28380435)
Nat Rev Genet. 2004 Jul;5(7):522-31. (PMID: 15211354)
FEBS J. 2013 May;280(9):2105-16. (PMID: 23480797)
Biochem J. 2007 Dec 15;408(3):317-26. (PMID: 17848139)
Mol Immunol. 2015 Dec;68(2 Pt B):476-83. (PMID: 26463158)
Chem Commun (Camb). 2013 May 14;49(39):4415-7. (PMID: 23111503)
Proc Natl Acad Sci U S A. 2012 Aug 14;109(33):13362-7. (PMID: 22853952)
Am J Physiol. 1994 May;266(5 Pt 1):L493-501. (PMID: 7515578)
Eur J Hum Genet. 2014 Jun;22(6):784-91. (PMID: 24129438)
Lab Invest. 2009 Jun;89(6):636-44. (PMID: 19333236)
J Biol Chem. 2003 Feb 28;278(9):7500-9. (PMID: 12446679)
Curr Pharm Des. 2010;16(28):3118-23. (PMID: 20687874)
Paediatr Respir Rev. 2018 Jan;25:64-72. (PMID: 28372929)
Eur Respir J. 2015 Jan;45(1):116-28. (PMID: 25186262)
Biochem J. 1997 Nov 1;327 ( Pt 3):651-62. (PMID: 9581539)
Curr Opin Struct Biol. 2005 Jun;15(3):331-41. (PMID: 15925505)
Oncogene. 2011 Aug 4;30(31):3440-53. (PMID: 21460854)
ChemMedChem. 2011 Dec 9;6(12):2192-202. (PMID: 22012891)
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D109-11. (PMID: 14681370)
Nature. 2005 Feb 17;433(7027):769-73. (PMID: 15685193)
Methods Mol Biol. 2014;1050:19-35. (PMID: 24297348)
Curr Pharm Des. 2001 Nov;7(17):1839-62. (PMID: 11562312)
J Immunol. 2013 Apr 1;190(7):3354-62. (PMID: 23436935)
Curr Med Chem. 2001 Apr;8(5):545-50. (PMID: 11281841)
Int J Oncol. 2012 Dec;41(6):2119-27. (PMID: 22992757)
Science. 1991 Dec 6;254(5037):1497-500. (PMID: 1962210)
Nucleic Acids Res. 2008 Aug;36(13):4424-32. (PMID: 18596083)
Dev Comp Immunol. 2017 Mar;68:79-86. (PMID: 27894672)
Proc Natl Acad Sci U S A. 2011 Nov 15;108(46):18843-8. (PMID: 21976485)
J Cyst Fibros. 2018 Mar;17(2S):S52-S60. (PMID: 28986017)
Cell. 2005 Jul 15;122(1):9-12. (PMID: 16009127)
PLoS One. 2011;6(10):e26601. (PMID: 22028919)
PLoS One. 2012;7(11):e50837. (PMID: 23226399)
Front Pharmacol. 2017 Jun 20;8:390. (PMID: 28676762)
Chem Biol. 2006 Nov;13(11):1235-42. (PMID: 17114005)
Nucleic Acids Res. 2010 Jul;38(13):4466-75. (PMID: 20223773)
Int J Cancer. 2014 Sep 15;135(6):1286-96. (PMID: 24510775)
Mol Ther. 2004 Oct;10(4):652-9. (PMID: 15451449)
J Neurooncol. 2014 May;118(1):19-28. (PMID: 24595467)
Biochem J. 2011 Aug 15;438(1):25-32. (PMID: 21689072)
Epigenomics. 2011 Dec;3(6):733-45. (PMID: 22126292)
Biochem Pharmacol. 2011 Nov 15;82(10):1416-29. (PMID: 21864506)
PLoS One. 2013;8(3):e60448. (PMID: 23555973)
J Cyst Fibros. 2013 Dec;12(6):797-802. (PMID: 23632450)
RNA. 2008 Feb;14(2):336-46. (PMID: 18073344)
Nature. 2015 Feb 5;518(7537):107-10. (PMID: 25409146)
J Biol Chem. 1995 Dec 1;270(48):28848-57. (PMID: 7499410)
Nature. 1993 Oct 7;365(6446):566-8. (PMID: 7692304)
J Clin Endocrinol Metab. 2016 Dec;101(12):4955-4963. (PMID: 27689251)
Curr Med Chem. 2004 May;11(10):1253-63. (PMID: 15134518)
Eur J Pharm Sci. 2015 Mar 10;69:1-9. (PMID: 25555374)
Development. 2005 Nov;132(21):4653-62. (PMID: 16224045)
فهرسة مساهمة: Keywords: CFTR; cystic fibrosis; delivery; miR-145; miRNA targeting; microRNAs; peptide nucleic acids
المشرفين على المادة: 0 (3' Untranslated Regions)
0 (MIRN145 microRNA, human)
0 (MicroRNAs)
0 (Peptide Nucleic Acids)
0 (RNA, Messenger)
126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator)
تواريخ الأحداث: Date Created: 20171230 Date Completed: 20180730 Latest Revision: 20181207
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6017273
DOI: 10.3390/molecules23010071
PMID: 29286300
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules23010071