دورية أكاديمية
A Peptide Nucleic Acid against MicroRNA miR-145-5p Enhances the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells.
العنوان: | A Peptide Nucleic Acid against MicroRNA miR-145-5p Enhances the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells. |
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المؤلفون: | Fabbri E; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. enrica.fabbri@unife.it., Tamanini A; Laboratory of Molecular Pathology, University-Hospital, 37126 Verona, Italy. anna.tamanini@aovr.veneto.it., Jakova T; Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, Italy. tiziana.jakova@studenti.unipr.it., Gasparello J; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. gspjsc@unife.it., Manicardi A; Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, Italy. Alex.manicardi@ugent.be., Corradini R; Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, Italy. roberto.corradini@unipr.it., Sabbioni G; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. giuseppe.sabbioni@student.unife.it., Finotti A; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. alessia.finotti@unife.it., Borgatti M; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. monica.borgatti@unife.it., Lampronti I; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. ilaria.lampronti@unife.it., Munari S; Laboratory of Molecular Pathology, University-Hospital, 37126 Verona, Italy. silvia.munari88@gmail.com., Dechecchi MC; Laboratory of Molecular Pathology, University-Hospital, 37126 Verona, Italy. cristina.dechecchi@ospedaleuniverona.it., Cabrini G; Laboratory of Molecular Pathology, University-Hospital, 37126 Verona, Italy. giulio.cabrini@univr.it., Gambari R; Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. gam@unife.it. |
المصدر: | Molecules (Basel, Switzerland) [Molecules] 2017 Dec 29; Vol. 23 (1). Date of Electronic Publication: 2017 Dec 29. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, c1995- |
مواضيع طبية MeSH: | Cystic Fibrosis Transmembrane Conductance Regulator/*metabolism , Gene Expression Regulation/*drug effects , MicroRNAs/*metabolism , Peptide Nucleic Acids/*pharmacology, 3' Untranslated Regions/genetics ; Apoptosis/drug effects ; Base Sequence ; Binding Sites ; Cell Line ; Cell Proliferation/drug effects ; Cystic Fibrosis Transmembrane Conductance Regulator/genetics ; Evolution, Molecular ; Humans ; MicroRNAs/genetics ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Up-Regulation/drug effects |
مستخلص: | Peptide nucleic acids (PNAs) are very useful tools for gene regulation at different levels, but in particular in the last years their use for targeting microRNA (anti-miR PNAs) has provided impressive advancements. In this respect, microRNAs related to the repression of cystic fibrosis transmembrane conductance regulator (CFTR) gene, which is defective in cystic fibrosis, are of great importance in the development of new type of treatments. In this paper we propose the use of an anti-miR PNA for targeting miR-145, a microRNA reported to suppress CFTR expression. Octaarginine-anti-miR PNA conjugates were delivered to Calu-3 cells, exerting sequence dependent targeting of miR-145-5p. This allowed to enhance expression of the miR-145 regulated CFTR gene, analyzed at mRNA (RT-qPCR, Reverse Transcription quantitative Polymerase Chain Reaction) and CFTR protein (Western blotting) level. Competing Interests: The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results. |
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فهرسة مساهمة: | Keywords: CFTR; cystic fibrosis; delivery; miR-145; miRNA targeting; microRNAs; peptide nucleic acids |
المشرفين على المادة: | 0 (3' Untranslated Regions) 0 (MIRN145 microRNA, human) 0 (MicroRNAs) 0 (Peptide Nucleic Acids) 0 (RNA, Messenger) 126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator) |
تواريخ الأحداث: | Date Created: 20171230 Date Completed: 20180730 Latest Revision: 20181207 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC6017273 |
DOI: | 10.3390/molecules23010071 |
PMID: | 29286300 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1420-3049 |
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DOI: | 10.3390/molecules23010071 |