دورية أكاديمية
Purification and characterization of a protease from the visceral mass of Mytella charruana and its evaluation to obtain antimicrobial peptides.
| العنوان: | Purification and characterization of a protease from the visceral mass of Mytella charruana and its evaluation to obtain antimicrobial peptides. |
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| المؤلفون: | Dornelles LP; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., Deodato de Souza MF; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., da Silva PM; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., Procópio TF; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., Filho RSR; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., de Albuquerque Lima T; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., de Oliveira APS; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., Zingali RB; Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Paiva PMG; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., Pontual EV; Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, Brazil., Napoleão TH; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil. Electronic address: thiago.napoleao@ufpe.br. |
| المصدر: | Food chemistry [Food Chem] 2018 Apr 15; Vol. 245, pp. 1169-1175. Date of Electronic Publication: 2017 Nov 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Barking : Elsevier Applied Science Publishers Original Publication: Barking, Eng., Applied Science Publishers. |
| مواضيع طبية MeSH: | Anti-Bacterial Agents/*pharmacology , Antifungal Agents/*pharmacology , Bivalvia/*enzymology , Peptides/*pharmacology , Serine Endopeptidases/*metabolism, Animals ; Anti-Bacterial Agents/chemistry ; Antifungal Agents/chemistry ; Caseins/chemistry ; Caseins/metabolism ; Drug Evaluation, Preclinical/methods ; Electrophoresis, Polyacrylamide Gel ; Enzyme Stability ; Hydrogen-Ion Concentration ; Hydrolysis ; Microbial Sensitivity Tests ; Peptides/chemistry ; Peptides/metabolism ; Serine Endopeptidases/isolation & purification ; Temperature ; Viscera/enzymology |
| مستخلص: | This work describes purification of a protease from the visceral mass of the mussel Mytella charruana as well as evaluation of its ability to hydrolyze milk casein to generate antimicrobial peptides. The enzyme showed pI of 4.1 and a single polypeptide band of 83.1 kDa after SDS-PAGE. Sequence similarities with tropomyosin and myosin from mollusks were detected. The protease showed a trypsin-like activity with optimal temperature of 40 °C and stability in a wide pH range (3.0-9.0). K (Copyright © 2017 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antibacterial activity; Bioactive peptides; Bivalve mollusk; Casein; Protease |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Antifungal Agents) 0 (Caseins) 0 (Peptides) 0 (trypsin-like serine protease) EC 3.4.21.- (Serine Endopeptidases) |
| تواريخ الأحداث: | Date Created: 20171231 Date Completed: 20180830 Latest Revision: 20181202 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.foodchem.2017.11.044 |
| PMID: | 29287337 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-7072 |
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| DOI: | 10.1016/j.foodchem.2017.11.044 |