دورية أكاديمية
Exploring the molecular complexity of Triatoma dimidiata sialome.
| العنوان: | Exploring the molecular complexity of Triatoma dimidiata sialome. |
|---|---|
| المؤلفون: | Santiago PB; Programa Pós-Graduação em Ciências Médicas, Faculty of Medicine, The University of Brasília, Brasília, Brazil., de Araújo CN; Programa Pós-Graduação em Ciências Médicas, Faculty of Medicine, The University of Brasília, Brasília, Brazil; Faculty of Ceilândia, The University of Brasília, Brasília, Brazil. Electronic address: cnunes@unb.br., Charneau S; Department of Cell Biology, The University of Brasília, Brasília, Brazil., Bastos IMD; Department of Cell Biology, The University of Brasília, Brasília, Brazil., Assumpção TCF; Vector Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, Rockville, United States., Queiroz RML; Department of Cell Biology, The University of Brasília, Brasília, Brazil., Praça YR; Programa Pós-Graduação em Ciências Médicas, Faculty of Medicine, The University of Brasília, Brasília, Brazil., Cordeiro TM; Department of Cell Biology, The University of Brasília, Brasília, Brazil., Garcia CHS; Department of Cell Biology, The University of Brasília, Brasília, Brazil., da Silva IG; Department of Parasitology, The University of Goiás, Brazil., Raiol T; Department of Cell Biology, The University of Brasília, Brasília, Brazil; Instituto Leônidas e Maria Deane - Fiocruz Amazônia, Manaus, AM, Brazil., Motta FN; Faculty of Ceilândia, The University of Brasília, Brasília, Brazil., de Araújo Oliveira JV; Department of Computer Science, The University of Brasília, Brasília, Brazil., de Sousa MV; Department of Cell Biology, The University of Brasília, Brasília, Brazil., Ribeiro JMC; Vector Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, Rockville, United States., de Santana JM; Programa Pós-Graduação em Ciências Médicas, Faculty of Medicine, The University of Brasília, Brasília, Brazil; Department of Cell Biology, The University of Brasília, Brasília, Brazil. |
| المصدر: | Journal of proteomics [J Proteomics] 2018 Mar 01; Vol. 174, pp. 47-60. Date of Electronic Publication: 2017 Dec 27. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101475056 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7737 (Electronic) Linking ISSN: 18743919 NLM ISO Abbreviation: J Proteomics Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | Salivary Glands/*chemistry , Triatoma/*chemistry, Animals ; Chagas Disease/transmission ; High-Throughput Nucleotide Sequencing ; Humans ; Insect Vectors/genetics ; Transcriptome/genetics ; Triatoma/genetics |
| مستخلص: | Triatoma dimidiata, a Chagas disease vector widely distributed along Central America, has great capability for domestic adaptation as the majority of specimens caught inside human dwellings or in peridomestic areas fed human blood. Exploring the salivary compounds that overcome host haemostatic and immune responses is of great scientific interest. Here, we provide a deeper insight into its salivary gland molecules. We used high-throughput RNA sequencing to examine in depth the T. dimidiata salivary gland transcriptome. From >51 million reads assembled, 92.21% are related to putative secreted proteins. Lipocalin is the most abundant gene family, confirming it is an expanded family in Triatoma genus salivary repertoire. Other putatively secreted members include phosphatases, odorant binding protein, hemolysin, proteases, protease inhibitors, antigen-5 and antimicrobial peptides. This work expands the previous set of functionally annotated sequences from T. dimidiata salivary glands available in NCBI from 388 to 3815. Additionally, we complemented the salivary analysis through proteomics (available data via ProteomeXchange with identifier PXD008510), disclosing the set complexity of 119 secreted proteins and validating the transcriptomic results. Our large-scale approach enriches the pharmacologically active molecules database and improves our knowledge about the complexity of salivary compounds from haematophagous vectors and their biological interactions. Significance: Several haematophagous triatomine species can transmit Trypanosoma cruzi, the etiological agent of Chagas disease. Due to the reemergence of this disease, new drugs for its prevention and treatment are considered priorities. For this reason, the knowledge of vector saliva emerges as relevant biological finding, contributing to the design of different strategies for vector control and disease transmission. Here we report the transcriptomic and proteomic compositions of the salivary glands (sialome) of the reduviid bug Triatoma dimidiata, a relevant Chagas disease vector in Central America. Our results are robust and disclosed unprecedented insights into the notable diversity of its salivary glands content, revealing relevant anti-haemostatic salivary gene families. Our work expands almost ten times the previous set of functionally annotated sequences from T. dimidiata salivary glands available in NCBI. Moreover, using an integrated transcriptomic and proteomic approach, we showed a correlation pattern of transcription and translation processes for the main gene families found, an important contribution to the research of triatomine sialomes. Furthermore, data generated here reinforces the secreted proteins encountered can greatly contribute for haematophagic habit, Trypanosoma cruzi transmission and development of therapeutic agent studies. (Copyright © 2017 Elsevier B.V. All rights reserved.) |
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| معلومات مُعتمدة: | Z01 AI000810 United States Intramural NIH HHS; ZIA AI000810-18 United States Intramural NIH HHS |
| فهرسة مساهمة: | Keywords: Chagas disease; Haematophagy; Sialome; Triatoma dimidiata; Triatominae; Vector biology |
| تواريخ الأحداث: | Date Created: 20171231 Date Completed: 20190710 Latest Revision: 20191220 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC5803406 |
| DOI: | 10.1016/j.jprot.2017.12.016 |
| PMID: | 29288089 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1876-7737 |
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| DOI: | 10.1016/j.jprot.2017.12.016 |