دورية أكاديمية
أعرض في EDS

Ratiometric sweat secretion optical test in cystic fibrosis, carriers and healthy subjects.

التفاصيل البيبلوغرافية
العنوان: Ratiometric sweat secretion optical test in cystic fibrosis, carriers and healthy subjects.
المؤلفون: Bergamini G; Department of Medicine, Section of General Pathology, University of Verona, Verona, Italy., Tridello G; Cystic Fibrosis Centre, Azienda Ospedaliera Universitaria Integrata Verona, Verona, Italy., Calcaterra E; Department of Medicine, Section of General Pathology, University of Verona, Verona, Italy., Ceri S; Department of Electronics, Computer Science and Engineering and Bioengineering, Politecnico di Milano, Milano, Italy., Tagliasacchi M; Department of Electronics, Computer Science and Engineering and Bioengineering, Politecnico di Milano, Milano, Italy., Bianchi F; Department of Electronics, Computer Science and Engineering and Bioengineering, Politecnico di Milano, Milano, Italy., Monti F; Department of Electronics, Computer Science and Engineering and Bioengineering, Politecnico di Milano, Milano, Italy., Masciadri A; Department of Electronics, Computer Science and Engineering and Bioengineering, Politecnico di Milano, Milano, Italy., Laudanna E; Cystic Fibrosis Centre, Azienda Ospedaliera Universitaria Integrata Verona, Verona, Italy., Peserico D; Cystic Fibrosis Centre, Azienda Ospedaliera Universitaria Integrata Verona, Verona, Italy., Sorio E; Department of Medicine, Section of General Pathology, University of Verona, Verona, Italy., Esposito V; Department of Medicine, Section of General Pathology, University of Verona, Verona, Italy., Leal T; Louvain Centre for Toxicology and Applied Pharmacology (LTAP), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, Brussels, Belgium., Assael BM; Cystic Fibrosis Centre, Azienda Ospedaliera Universitaria Integrata Verona, Verona, Italy; Department of Pathophysiology and Transplantation, University of Milan, Internal Medicine Department, Respiratory Unit and Cystic Fibrosis Adult Center, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy., Sorio C; Department of Medicine, Section of General Pathology, University of Verona, Verona, Italy., Melotti P; Cystic Fibrosis Centre, Azienda Ospedaliera Universitaria Integrata Verona, Verona, Italy. Electronic address: paola.melotti@aovr.veneto.it.
المصدر: Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society [J Cyst Fibros] 2018 Mar; Vol. 17 (2), pp. 186-189. Date of Electronic Publication: 2017 Dec 29.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101128966 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-5010 (Electronic) Linking ISSN: 15691993 NLM ISO Abbreviation: J Cyst Fibros Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam ; New York : Elsevier, c2002-
مواضيع طبية MeSH: Optical Imaging*, Cystic Fibrosis/*diagnostic imaging , Cystic Fibrosis/*physiopathology , Sweat Glands/*physiopathology , Sweating/*physiology, Case-Control Studies ; Humans ; Reproducibility of Results ; Sensitivity and Specificity
مستخلص: We have simplified the published procedure (5) for measuring sweat rates in individual human sweat glands. Sweat secretion rates were obtained from sweat drops secreted on the forearm by multiple individual glands. We computed a ratio between CFTR-dependent (by intradermal microinjection of a β adrenergic cocktail) and CFTR-independent (by methacoline as cholinergic stimulus) sweat secretion rates. We obtained a reproducible, approximately linear readout of CFTR function with measurements performed by two different independent teams. We considered three groups (CF subjects, CF carriers and non-CF controls, n=22 in each group); their mean ratios was respectively 0.000, 0.104 and 0.205 The average ratio of CF subjects was consistent with diagnosis in 3 additional cases clinically resembling CF. All groups were clearly discriminated, with sensibility and specificity ranging from 82% to 100%. A software was developed for detecting sweat droplets. This bioassay is suitabile for multicentre studies focusing on CFTR targeted therapies, controversial diagnosis and functional relevance of rare CFTR mutations.
(Copyright © 2017 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Cystic fibrosis; Cystic fibrosis transmembrane conductance regulator; Image analysis; Sweat glands; Sweat rate
تواريخ الأحداث: Date Created: 20180103 Date Completed: 20191112 Latest Revision: 20220321
رمز التحديث: 20221213
DOI: 10.1016/j.jcf.2017.12.003
PMID: 29292091
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-5010
DOI:10.1016/j.jcf.2017.12.003