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Binding targets of termiticidal lectins from the bark and leaf of Myracrodruon urundeuva in the gut of Nasutitermes corniger workers.

التفاصيل البيبلوغرافية
العنوان: Binding targets of termiticidal lectins from the bark and leaf of Myracrodruon urundeuva in the gut of Nasutitermes corniger workers.
المؤلفون: Lima TA; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., Dornelles LP; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., Oliveira APS; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., Guedes CC; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., Souza SO; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., Sá RA; Centro Acadêmico do Agreste, Universidade Federal de Pernambuco, Caruaru, Brazil., Zingali RB; Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Napoleão TH; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil., Paiva PM; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil.
المصدر: Pest management science [Pest Manag Sci] 2018 Jul; Vol. 74 (7), pp. 1593-1599. Date of Electronic Publication: 2018 Feb 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Published for SCI by Wiley Country of Publication: England NLM ID: 100898744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4998 (Electronic) Linking ISSN: 1526498X NLM ISO Abbreviation: Pest Manag Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: West Sussex, UK : Published for SCI by Wiley, c2000-
مواضيع طبية MeSH: Anacardiaceae/*chemistry , Insecticides/*metabolism , Isoptera/*drug effects , Plant Lectins/*metabolism, Animals ; Digestive System/drug effects ; Digestive System/enzymology ; Isoptera/enzymology ; Plant Bark/chemistry ; Plant Leaves/chemistry ; Plant Lectins/administration & dosage
مستخلص: Background: Lectins, carbohydrate-binding proteins, from the bark (MuBL) and leaf (MuLL) of Myracrodruon urundeuva are termiticidal agents against Nasutitermes corniger workers and have been shown to induce oxidative stress and cell death in the midgut of these insects. In this study, we investigated the binding targets of MuBL and MuLL in the gut of N. corniger workers by determining the effects of these lectins on the activity of digestive enzymes. In addition, we used mass spectrometry to identify peptides from gut proteins that adsorbed to MuBL-Sepharose and MuLL-Sepharose columns.
Results: Exoglucanase activity was neutralized in the presence of MuBL and stimulated by MuLL. α-l-Arabinofuranosidase activity was not affected by MuBL but was inhibited by MuLL. Both lectins stimulated α-amylase activity and inhibited protease and trypsin-like activities. Peptides with homology to apolipophorin, trypsin-like enzyme, and ABC transporter substrate-binding protein were detected from proteins that adsorbed to MuBL-Sepharose, while peptides from proteins that bound to MuLL-Sepharose shared homology with apolipophorin.
Conclusion: This study revealed that digestive enzymes and transport proteins found in worker guts can be recognized by MuBL and MuLL. Thus, the mechanism of their termiticidal activity may involve changes in the digestion and absorption of nutrients. © 2018 Society of Chemical Industry.
(© 2018 Society of Chemical Industry.)
فهرسة مساهمة: Keywords: digestive enzymes; insecticidal activity; lectin affinity chromatography; plant lectins; transport proteins
المشرفين على المادة: 0 (Insecticides)
0 (Plant Lectins)
تواريخ الأحداث: Date Created: 20180104 Date Completed: 20181015 Latest Revision: 20181015
رمز التحديث: 20240628
DOI: 10.1002/ps.4847
PMID: 29297969
قاعدة البيانات: MEDLINE
الوصف
تدمد:1526-4998
DOI:10.1002/ps.4847