دورية أكاديمية
Stabilization of Human Tyrosine Hydroxylase in Maltodextrin Nanoparticles for Delivery to Neuronal Cells and Tissue.
| العنوان: | Stabilization of Human Tyrosine Hydroxylase in Maltodextrin Nanoparticles for Delivery to Neuronal Cells and Tissue. |
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| المؤلفون: | Bezem MT, Johannessen FG, Jung-Kc K, Gundersen ET, Jorge-Finnigan A, Ying M, Betbeder D; LIRIC - Lille Inflammation Research International Center - U995, University of Lille , and Inserm, CHU Lille, F-59000 Lille, France.; University of Artois, 62000 Arras, France., Herfindal L, Martinez A |
| المصدر: | Bioconjugate chemistry [Bioconjug Chem] 2018 Feb 21; Vol. 29 (2), pp. 493-502. Date of Electronic Publication: 2018 Jan 31. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 9010319 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-4812 (Electronic) Linking ISSN: 10431802 NLM ISO Abbreviation: Bioconjug Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Chemical Society, c1990- |
| مواضيع طبية MeSH: | Brain/*metabolism , Drug Carriers/*chemistry , Nanoparticles/*chemistry , Neurons/*metabolism , Polysaccharides/*chemistry , Tyrosine 3-Monooxygenase/*administration & dosage , Tyrosine 3-Monooxygenase/*pharmacokinetics, Animals ; Cell Line ; Enzyme Stability ; Enzyme Therapy ; Female ; Humans ; Male ; Models, Molecular ; Parkinson Disease/drug therapy ; Parkinson Disease/enzymology |
| مستخلص: | Enzyme replacement therapy (ERT) is a therapeutic approach envisioned decades ago for the correction of genetic disorders, but ERT has been less successful for the correction of disorders with neurological manifestations. In this work, we have tested the functionality of nanoparticles (NP) composed of maltodextrin with a lipid core to bind and stabilize tyrosine hydroxylase (TH). This is a complex and unstable brain enzyme that catalyzes the rate-limiting step in the synthesis of dopamine and other catecholamine neurotransmitters. We have characterized these TH-loaded NPs to evaluate their potential for ERT in diseases associated with TH dysfunction. Our results show that TH can be loaded into the lipid core maltodextrin NPs with high efficiency, and both stability and activity are maintained through loading and are preserved during storage. Binding to NPs also favored the uptake of TH to neuronal cells, both in cell culture and in the brain. The internalized NP-bound TH was active as we measured an increase in intracellular L-Dopa synthesis following NP uptake. Our approach seems promising for the use of catalytically active NPs in ERT to treat neurodegenerative and neuropsychiatric disorders characterized by dopamine deficiency, notably Parkinson's disease. |
| المشرفين على المادة: | 0 (Drug Carriers) 0 (Polysaccharides) 7CVR7L4A2D (maltodextrin) EC 1.14.16.2 (Tyrosine 3-Monooxygenase) |
| تواريخ الأحداث: | Date Created: 20180105 Date Completed: 20180917 Latest Revision: 20180917 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1021/acs.bioconjchem.7b00807 |
| PMID: | 29299922 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-4812 |
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| DOI: | 10.1021/acs.bioconjchem.7b00807 |