دورية أكاديمية
أعرض في EDS

Influence of cooling temperature in sperm subpopulations of domestic cats.

التفاصيل البيبلوغرافية
العنوان: Influence of cooling temperature in sperm subpopulations of domestic cats.
المؤلفون: Souza AK; Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina, PR, 86051-990 Brazil., Trautwein LGC; Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina, PR, 86051-990 Brazil., Paranzini CS; Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina, PR, 86051-990 Brazil., Perencin FM; Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina, PR, 86051-990 Brazil., Cardoso GS; Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina, PR, 86051-990 Brazil., Martins MIM; Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina, PR, 86051-990 Brazil. Electronic address: imartins@uel.br.
المصدر: Animal reproduction science [Anim Reprod Sci] 2018 Feb; Vol. 189, pp. 1-10. Date of Electronic Publication: 2017 Dec 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7807205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2232 (Electronic) Linking ISSN: 03784320 NLM ISO Abbreviation: Anim Reprod Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier Scientific Pub. Co.
مواضيع طبية MeSH: Cats/*physiology , Semen Preservation/*veterinary , Spermatozoa/*physiology, Animals ; Cold Temperature ; Image Processing, Computer-Assisted ; Male ; Refrigeration ; Semen Analysis
مستخلص: The objective of this study was to identify and compare domestic feline sperm subpopulations, chilled at -1 °C for 24 and 48 h, as well as to analyze the sperm frequency in different subpopulations. Ten adult cats were used. Sperm collection was performed using electroejaculation (EEJ). Spermatic kinetics were evaluated using a computerized system at three moments: fresh, 24 and 48 h after refrigeration. The ejaculates were divided into a group refrigerated at -1 °C (n = 5,) and a group refrigerated at 4 °C (n = 5),. A total of 1560 spermatozoa were analyzed individually, and the sperm subpopulations were identified using multivariate statistics. Three spermatic subpopulations were defined using prior analysis of the hierarchical dendrogram. A principal components analysis (PCA) identified the existence of three groups with higher iterations at the three moments: PC1 (VAP, VCL, VSL, ALH, SVI), PC2 (STR, LIN, WOB and SMI) and PC3 (BCF). Subpopulation 1, after 48 h of refrigeration at -1 °C, and subpopulation 3, after 24 h of refrigeration at 4 °C, maintained their sperm quality, which allowed us to characterize the groups of spermatozoa that were resistant to cryopreservation. The present study identified three well defined ejaculate spermatozoa subpopulations, with proportional distributions between the groups and two refrigeration resistant subpopulations.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Cryopreservation; Feline; Multivariate statistics; Spermatic kinetics; Spermatic subpopulation
تواريخ الأحداث: Date Created: 20180106 Date Completed: 20180823 Latest Revision: 20180823
رمز التحديث: 20240628
DOI: 10.1016/j.anireprosci.2017.12.015
PMID: 29301674
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2232
DOI:10.1016/j.anireprosci.2017.12.015