دورية أكاديمية
Context-Dependent Requirements for FimH and Other Canonical Virulence Factors in Gut Colonization by Extraintestinal Pathogenic Escherichia coli.
| العنوان: | Context-Dependent Requirements for FimH and Other Canonical Virulence Factors in Gut Colonization by Extraintestinal Pathogenic Escherichia coli. |
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| المؤلفون: | Russell CW; University of Utah School of Medicine, Department of Pathology, Division of Microbiology and Immunology, Salt Lake City, Utah, USA., Fleming BA; University of Utah School of Medicine, Department of Pathology, Division of Microbiology and Immunology, Salt Lake City, Utah, USA., Jost CA; University of Utah School of Medicine, Department of Pathology, Division of Microbiology and Immunology, Salt Lake City, Utah, USA., Tran A; University of Utah School of Medicine, Department of Pathology, Division of Microbiology and Immunology, Salt Lake City, Utah, USA., Stenquist AT; University of Utah School of Medicine, Department of Pathology, Division of Microbiology and Immunology, Salt Lake City, Utah, USA., Wambaugh MA; University of Utah School of Medicine, Department of Pathology, Division of Microbiology and Immunology, Salt Lake City, Utah, USA., Bronner MP; Department of Pathology, ARUP Laboratories, University of Utah, Salt Lake City, Utah, USA.; Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA., Mulvey MA; University of Utah School of Medicine, Department of Pathology, Division of Microbiology and Immunology, Salt Lake City, Utah, USA mulvey@path.utah.edu. |
| المصدر: | Infection and immunity [Infect Immun] 2018 Feb 20; Vol. 86 (3). Date of Electronic Publication: 2018 Feb 20 (Print Publication: 2018). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0246127 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5522 (Electronic) Linking ISSN: 00199567 NLM ISO Abbreviation: Infect Immun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : American Society For Microbiology Original Publication: [Bethesda, Md.] American Society for Microbiology. |
| مواضيع طبية MeSH: | Adhesins, Escherichia coli/*metabolism , Escherichia coli Infections/*microbiology , Extraintestinal Pathogenic Escherichia coli/*metabolism , Fimbriae Proteins/*metabolism , Gastrointestinal Tract/*microbiology , Virulence Factors/*metabolism, Adhesins, Escherichia coli/genetics ; Animals ; Extraintestinal Pathogenic Escherichia coli/genetics ; Female ; Fimbriae Proteins/genetics ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Virulence Factors/genetics |
| مستخلص: | Extraintestinal pathogenic Escherichia coli (ExPEC) acts as a commensal within the mammalian gut but can induce pathology upon dissemination to other host environments such as the urinary tract and bloodstream. ExPEC genomes are likely shaped by evolutionary forces encountered within the gut, where the bacteria spend much of their time, provoking the question of how their extraintestinal virulence traits arose. The principle of coincidental evolution, in which a gene that evolved in one niche happens to be advantageous in another, has been used to argue that ExPEC virulence factors originated in response to selective pressures within the gut ecosystem. As a test of this hypothesis, the fitness of ExPEC mutants lacking canonical virulence factors was assessed within the intact murine gut in the absence of antibiotic treatment. We found that most of the tested factors, including cytotoxic necrotizing factor type 1 (CNF1), Usp, colibactin, flagella, and plasmid pUTI89, were dispensable for gut colonization. The deletion of genes encoding the adhesin PapG or the toxin HlyA had transient effects but did not interfere with longer-term persistence. In contrast, a mutant missing the type 1 pilus-associated adhesin FimH displayed somewhat reduced persistence within the gut. However, this phenotype varied dependent on the presence of specific competing strains and was partially attributable to aberrant flagellin expression in the absence of fimH These data indicate that FimH and other key ExPEC-associated factors are not strictly required for gut colonization, suggesting that the development of extraintestinal virulence traits is not driven solely by selective pressures within the gut. (Copyright © 2018 Russell et al.) |
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| معلومات مُعتمدة: | R01 AI095647 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: Escherichia coli; ExPEC; FimH; colonization; evolution; intestinal; microbiota; mouse model; uropathogenic; virulence factors |
| المشرفين على المادة: | 0 (Adhesins, Escherichia coli) 0 (Virulence Factors) 0 (fimH protein, E coli) 147680-16-8 (Fimbriae Proteins) |
| تواريخ الأحداث: | Date Created: 20180110 Date Completed: 20180919 Latest Revision: 20240213 |
| رمز التحديث: | 20240213 |
| مُعرف محوري في PubMed: | PMC5820936 |
| DOI: | 10.1128/IAI.00746-17 |
| PMID: | 29311232 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5522 |
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| DOI: | 10.1128/IAI.00746-17 |