دورية أكاديمية
MicroRNA-34a promotes cell cycle arrest and apoptosis and suppresses cell adhesion by targeting DUSP1 in osteosarcoma.
| العنوان: | MicroRNA-34a promotes cell cycle arrest and apoptosis and suppresses cell adhesion by targeting DUSP1 in osteosarcoma. |
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| المؤلفون: | Gang L; Department of Orthopedics, The First Affiliated Hospital of Soochow UniversitySuzhou, China.; Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical UniversityHuai'an, China., Qun L; Department of Orthopaedics, People's Hospital of Lishui DistrictNanjing, China., Liu WD; Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical UniversityHuai'an, China., Li YS; Department of Rheumatology and Immunology, Huai'an First People's Hospital, Nanjing Medical UniversityHuai'an, China., Xu YZ; Department of Orthopedics, The First Affiliated Hospital of Soochow UniversitySuzhou, China., Yuan DT; Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical UniversityHuai'an, China. |
| المصدر: | American journal of translational research [Am J Transl Res] 2017 Dec 15; Vol. 9 (12), pp. 5388-5399. Date of Electronic Publication: 2017 Dec 15 (Print Publication: 2017). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: e-Century Pub. Corp Country of Publication: United States NLM ID: 101493030 Publication Model: eCollection Cited Medium: Print ISSN: 1943-8141 (Print) Linking ISSN: 19438141 NLM ISO Abbreviation: Am J Transl Res Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Madison, WI : e-Century Pub. Corp. |
| مستخلص: | MicroRNAs are often deregulated in most cancer types and have important functions in carcinogenesis and cancer progression. Here, we studied the function of microRNA-34 (miR-34a) in osteosarcoma MG63 and U-2OS cells by expressed with pre-miR-34a, anti-miR-34a and corresponding negative controls, respectively. Cells proliferation, cell cycle and apoptosis was measured by MTT and flow cytometry assay. The effect of miR-34a on DUSP1 expression was evaluated by luciferase assays, real-time PCR and western blot assay. The data showed that miR-34a reduced the proliferation of MG63 cells through prompting cell cycle arrest at G0/G1 phase, cell apoptosis, and suppressed cell adhesion ability. Whereas anti-miR-34a increases U-2OS cell proliferation by preventing cell apoptosis, and promotes cell adhesion. Finally, we identified Dual-specificity phosphatase 1 (DUSP1) as the target gene of miR-34a in osteosarcoma cells and confirmed that DUSP1 enhanced the proliferation through inhibiting cell cycle arrest at G0/G1 phase and apoptosis, and inhibits the decreased cell adhesion induced by miR-34a. However, inhibition of DUSP1 resulted in substantially decreased proliferation and adhesion, and cell cycle arrest in G0/G1 phase and cell apoptosis similar to that observed with miR-34a in U-2OS cells. Our findings find out an important function of miR-34a as a novel tumor-suppressor in osteosarcoma pathogenesis through inhibition of DUSP1. Competing Interests: None. |
| References: | Cancer Metastasis Rev. 2008 Jun;27(2):253-61. (PMID: 18330678) Oncotarget. 2017 May 16;8(20):33064-33077. (PMID: 28380435) Cell Physiol Biochem. 2016;38(6):2173-82. (PMID: 27185245) Oncotarget. 2014 Mar 15;5(5):1304-14. (PMID: 24675421) J Clin Lab Anal. 2017 Apr 4;:null. (PMID: 28374902) Oncotarget. 2015 Jul 30;6(21):18389-405. (PMID: 26158762) Mol Carcinog. 2017 Jun;56(6):1630-1641. (PMID: 28112450) Cancer. 2009 Apr 1;115(7):1531-43. (PMID: 19197972) Mol Med Rep. 2015 May;11(5):3301-7. (PMID: 25585539) Biosci Rep. 2014 Aug 06;34(4):null. (PMID: 24957404) J Clin Oncol. 2008 Jun 20;26(18):3102-3; author reply 3104-5. (PMID: 18565904) Tumour Biol. 2017 Apr;39(4):1010428317695932. (PMID: 28381192) Cancer Lett. 2014 Apr 28;346(1):114-21. (PMID: 24368190) Ann Surg Oncol. 2015 Feb;22(2):489-96. (PMID: 25155396) Oncol Res. 2017 Jul 5;25(6):989-999. (PMID: 28244849) PLoS One. 2012;7(3):e33778. (PMID: 22457788) Eur Rev Med Pharmacol Sci. 2017 Mar;21(5):928-936. (PMID: 28338204) Int J Med Sci. 2013;10(6):676-82. (PMID: 23569431) Tumour Biol. 2017 Mar;39(3):1010428317695920. (PMID: 28347239) Cell. 2004 Jan 23;116(2):281-97. (PMID: 14744438) Cancer Gene Ther. 2017 Feb;24(2):83-88. (PMID: 28186090) Int J Oncol. 2017 May;50(5):1461-1476. (PMID: 28393213) Pediatr Blood Cancer. 2008 Dec;51(6):754-9. (PMID: 18726921) Am J Transl Res. 2016 Aug 15;8(8):3503-12. (PMID: 27648140) Cancer Treat Rev. 2014 May;40(4):523-32. (PMID: 24345772) J Biol Chem. 2004 Mar 12;279(11):9882-91. (PMID: 14699150) Biochem Biophys Res Commun. 2009 Oct 9;388(1):35-40. (PMID: 19632201) Pediatr Blood Cancer. 2015 Jul;62(7):1209-13. (PMID: 25755160) Cancer Metastasis Rev. 2007 Dec;26(3-4):579-85. (PMID: 17717636) Nat Rev Drug Discov. 2010 Oct;9(10):775-89. (PMID: 20885409) J Agric Food Chem. 2017 Apr 5;65(13):2670-2676. (PMID: 28301149) |
| فهرسة مساهمة: | Keywords: DUSP1; G0-G1 phase; adhesion; miR-34a; osteosarcoma |
| تواريخ الأحداث: | Date Created: 20180110 Latest Revision: 20220331 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC5752889 |
| PMID: | 29312491 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1943-8141 |
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