دورية أكاديمية
Skin corrosion test: a comparison between reconstructed human epidermis and full thickness skin models.
| العنوان: | Skin corrosion test: a comparison between reconstructed human epidermis and full thickness skin models. |
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| المؤلفون: | Catarino CM; Department of Chemical and Biological Engineer, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110, 8th Street, 12180 Troy, NY, United States; Clinical and Toxicological Analyses Department, School of Pharmaceutical Sciences, University of São Paulo, Av. Professor Lineu Prestes, 580, Bloco 17, 05508-000 São Paulo, São Paulo, Brazil., do Nascimento Pedrosa T; Clinical and Toxicological Analyses Department, School of Pharmaceutical Sciences, University of São Paulo, Av. Professor Lineu Prestes, 580, Bloco 17, 05508-000 São Paulo, São Paulo, Brazil., Pennacchi PC; Clinical and Toxicological Analyses Department, School of Pharmaceutical Sciences, University of São Paulo, Av. Professor Lineu Prestes, 580, Bloco 17, 05508-000 São Paulo, São Paulo, Brazil., de Assis SR; Clinical and Toxicological Analyses Department, School of Pharmaceutical Sciences, University of São Paulo, Av. Professor Lineu Prestes, 580, Bloco 17, 05508-000 São Paulo, São Paulo, Brazil., Gimenes F; Department of Clinical Analysis and Biomedicine, State University of Maringá, Avenida Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil., Consolaro MEL; Department of Clinical Analysis and Biomedicine, State University of Maringá, Avenida Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil., de Moraes Barros SB; Clinical and Toxicological Analyses Department, School of Pharmaceutical Sciences, University of São Paulo, Av. Professor Lineu Prestes, 580, Bloco 17, 05508-000 São Paulo, São Paulo, Brazil., Maria-Engler SS; Clinical and Toxicological Analyses Department, School of Pharmaceutical Sciences, University of São Paulo, Av. Professor Lineu Prestes, 580, Bloco 17, 05508-000 São Paulo, São Paulo, Brazil. Electronic address: silvya@usp.br. |
| المصدر: | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2018 Apr; Vol. 125, pp. 51-57. Date of Electronic Publication: 2018 Jan 06. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 9109778 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3441 (Electronic) Linking ISSN: 09396411 NLM ISO Abbreviation: Eur J Pharm Biopharm Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science Original Publication: Stuttgart : Wissenschaftliche Verlagsgesellschaft, c1991- |
| مواضيع طبية MeSH: | Cell Survival/*drug effects , Epidermis/*drug effects , Irritants/*toxicity , Skin Irritancy Tests/*methods, Cell Survival/physiology ; Corrosion ; Epidermis/pathology ; Epidermis/physiology ; Humans ; Male ; Skin/drug effects ; Skin/pathology ; Skin Tests/methods |
| مستخلص: | Currently, there is a strong global trend towards the development of in vitro models to replace the use of animals in safety evaluation tests. Reconstructed Human Epidermis (RHE) models have been employed as an alternative method to animal testing of skin corrosion and irritation potential of chemical compounds. However, the consequences of an absence of the dermal compartment in these models should be considered since the cross-talk between fibroblasts and keratinocytes is fundamental for promoting proper epidermal stratification, homeostasis, inflammatory response and wound healing. In this study, we compare in-house developed models of Reconstructed Human Epidermis (i.e. USP-RHE) and full thickness skin (i.e. USP-FTS) regarding their response when submitted to skin corrosion assays, based on Guideline 431 (OECD). The results show that both models correctly classified the four substances tested (2-phenylethyl bromide, benzylacetone, lactic acid, octanoic acid) as corrosive or non-corrosive. Furthermore, we have demonstrated higher cell viability of the USP-FTS model compared to the USP-RHE model, a sign of its improved barrier function, following the exposure to the substances test on the corrosion assay. This emphasizes the importance of employing in vitro models that are more physiologically relevant and that better mimic the in vivo situation for the toxicological screening of substances. (Copyright © 2018 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Full thickness skin; Reconstructed human epidermis; Risk assessment; Skin corrosion |
| المشرفين على المادة: | 0 (Irritants) |
| تواريخ الأحداث: | Date Created: 20180111 Date Completed: 20180903 Latest Revision: 20180903 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.ejpb.2018.01.002 |
| PMID: | 29317274 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3441 |
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| DOI: | 10.1016/j.ejpb.2018.01.002 |