دورية أكاديمية
أعرض في EDS

The role of Hedgehog-responsive fibroblasts in facial nerve regeneration.

التفاصيل البيبلوغرافية
العنوان: The role of Hedgehog-responsive fibroblasts in facial nerve regeneration.
المؤلفون: Bobarnac Dogaru GL; Keck School of Medicine of the University of Southern California, 2250 Alcazar St - CSA 222, Los Angeles, CA 90033, United States., Juneja SC; Division of Facial Plastic & Reconstructive Surgery, Department of Otolaryngology - Head & Neck Surgery, Stanford University School of Medicine, 801 Welch Rd, Stanford, CA 94305, United States., Shokrani A; Division of Facial Plastic & Reconstructive Surgery, USC Caruso Department of Otolaryngology-Head & Neck Surgery, Keck School of Medicine of USC, 1540 Alcazar Street Ste 204M, Los Angeles, CA 90033, United States., Hui RY; Keck School of Medicine of the University of Southern California, 2250 Alcazar St - CSA 222, Los Angeles, CA 90033, United States; California Institute for Regenerative Medicine, United States; Pasadena City College, Pasadena, CA, United States., Chai Y; Center for Craniofacial Molecular Biology, Ostrow School of Dentistry of USC, 2250 Alcazar Street - CSA 103, Los Angeles, CA 90033, United States., Pepper JP; Division of Facial Plastic & Reconstructive Surgery, Department of Otolaryngology - Head & Neck Surgery, Stanford University School of Medicine, 801 Welch Rd, Stanford, CA 94305, United States; Division of Facial Plastic & Reconstructive Surgery, USC Caruso Department of Otolaryngology-Head & Neck Surgery, Keck School of Medicine of USC, 1540 Alcazar Street Ste 204M, Los Angeles, CA 90033, United States. Electronic address: jpepper@stanford.edu.
المصدر: Experimental neurology [Exp Neurol] 2018 May; Vol. 303, pp. 72-79. Date of Electronic Publication: 2018 Jan 11.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 0370712 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2430 (Electronic) Linking ISSN: 00144886 NLM ISO Abbreviation: Exp Neurol Subsets: MEDLINE
أسماء مطبوعة: Publication: Orlando Fl : Academic Press
Original Publication: New York.
مواضيع طبية MeSH: Facial Nerve Injuries/*pathology , Fibroblasts/*metabolism , Hedgehogs/*metabolism , Nerve Regeneration/*genetics , Zinc Finger Protein GLI1/*metabolism, Animals ; Antigens/metabolism ; Disease Models, Animal ; Fibronectins/metabolism ; Flow Cytometry ; Galactosides/genetics ; Galactosides/metabolism ; Gene Expression Regulation/genetics ; Hedgehogs/genetics ; Indoles/metabolism ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Neurons/metabolism ; Neurons/pathology ; Neurons/ultrastructure ; Proteoglycans/metabolism ; Receptor, Nerve Growth Factor/metabolism ; S100 Calcium Binding Protein beta Subunit/metabolism ; Signal Transduction/genetics ; Zinc Finger Protein GLI1/genetics
مستخلص: Background: Facial nerve paralysis is a significant cause of morbidity, affecting facial appearance, emotional expression, speech, oral competence, and vision. A more complete understanding of the complex cellular events required for successful nerve regeneration may reveal new therapeutic targets. The role of fibroblasts in regeneration, and the process by which the nerve reforms its three-dimensional structure after a transection injury, are not fully understood. The Hedgehog signaling pathway has been shown to mediate nerve sheath formation during development. We therefore sought to characterize the role of Hedgehog-responsive cells following transection of the facial nerve.
Methods: Two transgenic mouse lines with reporters for the downstream effector of Hedgehog signaling, Gli1, were used. The animals underwent a unilateral facial nerve transection injury, and the contralateral side served as a control. Facial nerves were analyzed via immunohistochemistry and immunofluorescence at predetermined time points as the facial nerve regenerated after the transection injury.
Results: There was a statistically significant increase in Gli1+ cells both at the site of injury and within the distal nerve segment over time. Gli1+ cells are fibroblasts within the nerve and appear to contribute to the reformation of the nerve sheath after injury.
Conclusion: These findings describe a key signaling pathway by which fibroblasts participate in motor nerve regeneration. Fibroblasts that reside within the nerve respond to injury and may represent a novel therapeutic target in the context of facial nerve regeneration after transection injury.
(Copyright © 2018. Published by Elsevier Inc.)
معلومات مُعتمدة: R37 DE012711 United States DE NIDCR NIH HHS; R01 DE025221 United States DE NIDCR NIH HHS
فهرسة مساهمة: Keywords: Facial nerve; Facial paralysis; Hedgehog signaling; Intraneural fibroblast; Nerve regeneration
المشرفين على المادة: 0 (Antigens)
0 (Fibronectins)
0 (Galactosides)
0 (Gli1 protein, mouse)
0 (Indoles)
0 (Luminescent Proteins)
0 (Proteoglycans)
0 (Receptor, Nerve Growth Factor)
0 (S100 Calcium Binding Protein beta Subunit)
0 (Zinc Finger Protein GLI1)
0 (chondroitin sulfate proteoglycan 4)
V595OG374W (5-bromo-4-chloro-3-indolyl beta-galactoside)
تواريخ الأحداث: Date Created: 20180117 Date Completed: 20181220 Latest Revision: 20191210
رمز التحديث: 20240628
DOI: 10.1016/j.expneurol.2018.01.008
PMID: 29337143
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2430
DOI:10.1016/j.expneurol.2018.01.008