دورية أكاديمية
Amino group of salicylic acid exhibits enhanced inhibitory potential against insulin amyloid fibrillation with protective aptitude toward amyloid induced cytotoxicity.
| العنوان: | Amino group of salicylic acid exhibits enhanced inhibitory potential against insulin amyloid fibrillation with protective aptitude toward amyloid induced cytotoxicity. |
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| المؤلفون: | Zaman M; Molecular Biophysics and Biophysical Chemistry Group, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India., Khan MV; Molecular Biophysics and Biophysical Chemistry Group, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India., Zakariya SM; Molecular Biophysics and Biophysical Chemistry Group, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India., Nusrat S; Molecular Biophysics and Biophysical Chemistry Group, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India., Meeran SM; Laboratory of Cancer Epigenetics, Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India., Alam P; Molecular Biophysics and Biophysical Chemistry Group, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India., Ajmal MR; Molecular Biophysics and Biophysical Chemistry Group, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India., Wahiduzzaman W; Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India., Shahein YE; Genetic Engineering and Biotechnology Division, Molecular Biology Department, National Research Centre, Dokki, Cairo, Egypt.; Biology Department, College of Science, Hail University, Hail, Saudi Arabia., Abouelella AM; Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Cairo, Egypt., Khan RH; Molecular Biophysics and Biophysical Chemistry Group, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India. |
| المصدر: | Journal of cellular biochemistry [J Cell Biochem] 2018 May; Vol. 119 (5), pp. 3945-3956. Date of Electronic Publication: 2018 Jan 19. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8205768 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4644 (Electronic) Linking ISSN: 07302312 NLM ISO Abbreviation: J Cell Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2004>- : Hoboken, NJ : Wiley-Liss Original Publication: New York : Liss, c1982- |
| مواضيع طبية MeSH: | Amyloid*/chemistry , Amyloid*/pharmacology , Insulin*/chemistry , Insulin*/pharmacology, Mesalamine/*chemistry , Salicylic Acid/*chemistry, Animals ; Cattle ; Cell Line, Tumor ; Humans |
| مستخلص: | Protein misfolding and aggregation lead to amyloid generation that in turn may induce cell membrane disruption and leads to cell apoptosis. In an effort to prevent or treat amyloidogenesis, large number of studies has been paying attention on breakthrough of amyloid inhibitors. In the present work, we aim to access the effect of two drugs, that is, acetylsalicylic acid and 5-amino salicylic acid on insulin amyloids by using various biophysical, imaging, cell viability assay, and computational approaches. We established that both drugs reduce the turbidity, light scattering and fluorescence intensity of amyloid indicator dye thioflavin T. Premixing of drugs with insulin inhibited the nucleation phase and inhibitory potential was boosted by increasing the concentration of the drug. Moreover, addition of drugs at the studied concentrations attenuated the insulin fibril induced cytotoxicity in breast cancer cell line MDA-MB-231. Our results highlight the amino group of salicylic acid exhibited enhanced inhibitory effects on insulin fibrillation in comparison to acetyl group. It may be due to presence of amino group that helps it to prolong the nucleation phase with strong binding as well as disruption of aromatic and hydrophobic stacking that plays a key role in amyloid progression. (© 2017 Wiley Periodicals, Inc.) |
| فهرسة مساهمة: | Keywords: 5-aminosalicycylic acid; acetylsalicylic acid; amyloid; cytotoxicity; inhibition |
| المشرفين على المادة: | 0 (Amyloid) 0 (Insulin) 4Q81I59GXC (Mesalamine) O414PZ4LPZ (Salicylic Acid) |
| تواريخ الأحداث: | Date Created: 20180120 Date Completed: 20181211 Latest Revision: 20181211 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1002/jcb.26538 |
| PMID: | 29350433 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1097-4644 |
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| DOI: | 10.1002/jcb.26538 |