دورية أكاديمية
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Identification of a biosynthetic gene cluster for the polyene macrolactam sceliphrolactam in a Streptomyces strain isolated from mangrove sediment.

التفاصيل البيبلوغرافية
العنوان: Identification of a biosynthetic gene cluster for the polyene macrolactam sceliphrolactam in a Streptomyces strain isolated from mangrove sediment.
المؤلفون: Low ZJ; School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Singapore., Pang LM; School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Singapore., Ding Y; Interdisciplinary Graduate School, Nanyang Technological University, Singapore, Singapore.; Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, 637551, Singapore, Singapore., Cheang QW; School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Singapore., Le Mai Hoang K; School of Mathematics and Physics, Nanyang Technological University, Singapore, 637551, Singapore., Thi Tran H; School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Singapore., Li J; Department of Bioinformatics, School of Basic Medical Sciences, Southern Medical University, 510515, Guangzhou, China., Liu XW; School of Mathematics and Physics, Nanyang Technological University, Singapore, 637551, Singapore., Kanagasundaram Y; Bioinformatics Institute, Singapore, 138671, Singapore., Yang L; School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Singapore.; Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, 637551, Singapore, Singapore., Liang ZX; School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Singapore. zxliang@ntu.edu.sg.
المصدر: Scientific reports [Sci Rep] 2018 Jan 25; Vol. 8 (1), pp. 1594. Date of Electronic Publication: 2018 Jan 25.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Multigene Family*, Biological Products/*metabolism , Biosynthetic Pathways/*genetics , Lactams, Macrocyclic/*metabolism , Streptomyces/*genetics , Streptomyces/*metabolism, Computational Biology ; Environmental Microbiology ; Gene Knockout Techniques ; Streptomyces/isolation & purification ; Tropical Climate ; Whole Genome Sequencing
مستخلص: Streptomyces are a genus of Actinobacteria capable of producing structurally diverse natural products. Here we report the isolation and characterization of a biosynthetically talented Streptomyces (Streptomyces sp. SD85) from tropical mangrove sediments. Whole-genome sequencing revealed that Streptomyces sp. SD85 harbors at least 52 biosynthetic gene clusters (BGCs), which constitute 21.2% of the 8.6-Mb genome. When cultivated under lab conditions, Streptomyces sp. SD85 produces sceliphrolactam, a 26-membered polyene macrolactam with unknown biosynthetic origin. Genome mining yielded a putative sceliphrolactam BGC (sce) that encodes a type I modular polyketide synthase (PKS) system, several β-amino acid starter biosynthetic enzymes, transporters, and transcriptional regulators. Using the CRISPR/Cas9-based gene knockout method, we demonstrated that the sce BGC is essential for sceliphrolactam biosynthesis. Unexpectedly, the PKS system encoded by sce is short of one module required for assembling the 26-membered macrolactam skeleton according to the collinearity rule. With experimental data disfavoring the involvement of a trans-PKS module, the biosynthesis of sceliphrolactam seems to be best rationalized by invoking a mechanism whereby the PKS system employs an iterative module to catalyze two successive chain extensions with different outcomes. The potential violation of the collinearity rule makes the mechanism distinct from those of other polyene macrolactams.
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المشرفين على المادة: 0 (Biological Products)
0 (Lactams, Macrocyclic)
0 (sceliphrolactam)
تواريخ الأحداث: Date Created: 20180127 Date Completed: 20181126 Latest Revision: 20190125
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC5785472
DOI: 10.1038/s41598-018-20018-8
PMID: 29371699
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-018-20018-8