دورية أكاديمية
أعرض في EDS

Variation in primary and culture-expanded cells derived from connective tissue progenitors in human bone marrow space, bone trabecular surface and adipose tissue.

التفاصيل البيبلوغرافية
العنوان: Variation in primary and culture-expanded cells derived from connective tissue progenitors in human bone marrow space, bone trabecular surface and adipose tissue.
المؤلفون: Qadan MA; Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA; School of Biomedical Sciences, Kent State University, Kent, Ohio, USA; Department of Biotechnology and Genetic Engineering, Philadelphia University, Amman, Jordan., Piuzzi NS; Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA; Department of Orthopaedic Surgery, Cleveland Clinic Foundation, Cleveland, Ohio, USA; Instituto Universitario del Hospital Italiano de Buenos Aires, Buenos Aires, Argentina., Boehm C; Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA., Bova W; Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA., Moos M Jr; FDA/Center for Biologics Evaluation and Research, Division of Cellular and Gene Therapies, Office of Cellular, Tissue, and Gene Therapies, Silver Spring, Maryland, USA., Midura RJ; Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA., Hascall VC; Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA., Malcuit C; School of Biomedical Sciences, Kent State University, Kent, Ohio, USA., Muschler GF; Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA; Department of Orthopaedic Surgery, Cleveland Clinic Foundation, Cleveland, Ohio, USA. Electronic address: muschlg@ccf.org.
المصدر: Cytotherapy [Cytotherapy] 2018 Mar; Vol. 20 (3), pp. 343-360. Date of Electronic Publication: 2018 Feb 01.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 100895309 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-2566 (Electronic) Linking ISSN: 14653249 NLM ISO Abbreviation: Cytotherapy Subsets: MEDLINE
أسماء مطبوعة: Publication: 2013- : London : Elsevier
Original Publication: Oxford, England : ISIS Medical Media, c1999-
مواضيع طبية MeSH: Adipose Tissue/*cytology , Biomarkers/*metabolism , Bone and Bones/*cytology , Connective Tissue Cells/*cytology , Stem Cells/*cytology, Adult ; Aged ; Bone Marrow Cells/cytology ; Cell Culture Techniques ; Cells, Cultured ; Connective Tissue Cells/physiology ; Female ; Humans ; Male ; Mesenchymal Stem Cells/metabolism ; Middle Aged ; Stem Cells/physiology
مستخلص: Background Aims: Connective tissue progenitors (CTPs) embody the heterogeneous stem and progenitor cell populations present in native tissue. CTPs are essential to the formation and remodeling of connective tissue and represent key targets for tissue-engineering and cell-based therapies. To better understand and characterize CTPs, we aimed to compare the (i) concentration and prevalence, (ii) early in vitro biological behavior and (iii) expression of surface-markers and transcription factors among cells derived from marrow space (MS), trabecular surface (TS), and adipose tissues (AT).
Methods: Cancellous-bone and subcutaneous-adipose tissues were collected from 8 patients. Cells were isolated and cultured. Colony formation was assayed using Colonyze software based on ASTM standards. Cell concentration ([Cell]), CTP concentration ([CTP]) and CTP prevalence (P CTP ) were determined. Attributes of culture-expanded cells were compared based on (i) effective proliferation rate and (ii) expression of surface-markers CD73, CD90, CD105, SSEA-4, SSEA-3, SSEA-1/CD15, Cripto-1, E-Cadherin/CD324, Ep-CAM/CD326, CD146, hyaluronan and transcription factors Oct3/4, Sox-2 and Nanog using flow cytometry.
Results: Mean [Cell], [CTP] and P CTP were significantly different between MS and TS samples (P = 0.03, P = 0.008 and P= 0.0003), respectively. AT-derived cells generated the highest mean total cell yield at day 6 of culture-4-fold greater than TS and more than 40-fold greater than MS per million cells plated. TS colonies grew with higher mean density than MS colonies (290 ± 11 versus 150 ± 11 cell per mm 2 ; P = 0.0002). Expression of classical-mesenchymal stromal cell (MSC) markers was consistently recorded (>95%) from all tissue sources, whereas all the other markers were highly variable.
Conclusions: The prevalence and biological potential of CTPs are different between patients and tissue sources and lack variation in classical MSC markers. Other markers are more likely to discriminate differences between cell populations in biological performance. Understanding the underlying reasons for variation in the concentration, prevalence, marker expression and biological potential of CTPs between patients and source tissues and determining the means of managing this variation will contribute to the rational development of cell-based clinical diagnostics and targeted cell-based therapies.
(Copyright © 2017 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: R21 AR067357 United States AR NIAMS NIH HHS
فهرسة مساهمة: Keywords: adipose tissue; bone; colony flow cytometry; connective tissue progenitor; forming unit assay; mesenchymal stromal cell; stem cell; surface markers; trabecular bone; transcription factor
المشرفين على المادة: 0 (Biomarkers)
تواريخ الأحداث: Date Created: 20180204 Date Completed: 20190520 Latest Revision: 20220331
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5847472
DOI: 10.1016/j.jcyt.2017.11.013
PMID: 29396254
قاعدة البيانات: MEDLINE
الوصف
تدمد:1477-2566
DOI:10.1016/j.jcyt.2017.11.013