دورية أكاديمية
Immunization of Malaria-Preexposed Volunteers With PfSPZ Vaccine Elicits Long-Lived IgM Invasion-Inhibitory and Complement-Fixing Antibodies.
| العنوان: | Immunization of Malaria-Preexposed Volunteers With PfSPZ Vaccine Elicits Long-Lived IgM Invasion-Inhibitory and Complement-Fixing Antibodies. |
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| المؤلفون: | Zenklusen I; Clinical Immunology Unit, Swiss Tropical and Public Health Institute, Basel, Switzerland.; University of Basel, Switzerland., Jongo S; Ifakara Health Institute, Clinical Trial Unit, Bagamoyo, Tanzania., Abdulla S; Ifakara Health Institute, Clinical Trial Unit, Bagamoyo, Tanzania., Ramadhani K; Ifakara Health Institute, Clinical Trial Unit, Bagamoyo, Tanzania., Lee Sim BK; Sanaria Inc., Rockville, Maryland., Cardamone H; Center for Infectious Disease Research, Seattle, Washington., Flannery EL; Center for Infectious Disease Research, Seattle, Washington., Nguyen T; Center for Infectious Disease Research, Seattle, Washington., Fishbaugher M; Center for Infectious Disease Research, Seattle, Washington., Steel RWJ; Center for Infectious Disease Research, Seattle, Washington., Betz W; Center for Infectious Disease Research, Seattle, Washington., Carmago N; Center for Infectious Disease Research, Seattle, Washington., Mikolajczak S; Center for Infectious Disease Research, Seattle, Washington., Kappe SHI; Center for Infectious Disease Research, Seattle, Washington.; Department of Global Health, University of Washington, Seattle., Hoffman SL; Sanaria Inc., Rockville, Maryland., Sack BK; Center for Infectious Disease Research, Seattle, Washington., Daubenberger C; Clinical Immunology Unit, Swiss Tropical and Public Health Institute, Basel, Switzerland.; University of Basel, Switzerland. |
| المصدر: | The Journal of infectious diseases [J Infect Dis] 2018 Apr 23; Vol. 217 (10), pp. 1569-1578. |
| نوع المنشور: | Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0413675 Publication Model: Print Cited Medium: Internet ISSN: 1537-6613 (Electronic) Linking ISSN: 00221899 NLM ISO Abbreviation: J Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2011- : Oxford : Oxford University Press Original Publication: 1904-2010 : Chicago, IL : University of Chicago Press |
| مواضيع طبية MeSH: | Antibodies, Protozoan/*immunology , Immunoglobulin M/*immunology , Malaria/*immunology , Malaria Vaccines/*immunology , Malaria, Falciparum/*immunology, Adult ; Antibody Formation/immunology ; Double-Blind Method ; Humans ; Immunization/methods ; Male ; Plasmodium falciparum/immunology ; Sporozoites/immunology ; Vaccination/methods ; Vaccines, Attenuated/immunology ; Volunteers ; Young Adult |
| مستخلص: | Background: The assessment of antibody responses after immunization with radiation-attenuated, aseptic, purified, cryopreserved Plasmodium falciparum sporozoites (Sanaria PfSPZ Vaccine) has focused on IgG isotype antibodies. Here, we aimed to investigate if P. falciparum sporozoite binding and invasion-inhibitory IgM antibodies are induced following immunization of malaria-preexposed volunteers with PfSPZ Vaccine. Methods: Using serum from volunteers immunized with PfSPZ, we measured vaccine-induced IgG and IgM antibodies to P. falciparum circumsporozoite protein (PfCSP) via ELISA. Function of this serum as well as IgM antibody fractions was measured via in vitro in an inhibition of sporozoite invasion assay. These IgM antibody fractions were also measured for binding to sporozoites by immunofluorescence assay and complement fixation on whole sporozoites. Results: We found that in addition to anti-PfCSP IgG, malaria-preexposed volunteers developed anti-PfCSP IgM antibodies after immunization with PfSPZ Vaccine and that these IgM antibodies inhibited P. falciparum sporozoite invasion of hepatocytes in vitro. These IgM plasma fractions also fixed complement to whole P. falciparum sporozoites. Conclusions: This is the first finding that PfCSP and P. falciparum sporozoite-binding IgM antibodies are induced following immunization of PfSPZ Vaccine in malaria-preexposed individuals and that IgM antibodies can inhibit P. falciparum sporozoite invasion into hepatocytes in vitro and fix complement on sporozoites. These findings indicate that the immunological assessment of PfSPZ Vaccine-induced antibody responses could be more sensitive if they include parasite-specific IgM in addition to IgG antibodies. Clinical Trials Registration: NCT02132299. |
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| معلومات مُعتمدة: | F32 AI114113 United States AI NIAID NIH HHS; R44 AI055229 United States AI NIAID NIH HHS |
| سلسلة جزيئية: | ClinicalTrials.gov NCT02132299 |
| المشرفين على المادة: | 0 (Antibodies, Protozoan) 0 (Immunoglobulin M) 0 (Malaria Vaccines) 0 (Vaccines, Attenuated) |
| تواريخ الأحداث: | Date Created: 20180214 Date Completed: 20190905 Latest Revision: 20190906 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC5913598 |
| DOI: | 10.1093/infdis/jiy080 |
| PMID: | 29438525 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1537-6613 |
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| DOI: | 10.1093/infdis/jiy080 |