دورية أكاديمية
Heteroassembled gold nanoparticles with sandwich-immunoassay LSPR chip format for rapid and sensitive detection of hepatitis B virus surface antigen (HBsAg).
| العنوان: | Heteroassembled gold nanoparticles with sandwich-immunoassay LSPR chip format for rapid and sensitive detection of hepatitis B virus surface antigen (HBsAg). |
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| المؤلفون: | Kim J; WCSL of Integrated Human Airway-on-a-Chip, Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, South Korea., Oh SY; WCSL of Integrated Human Airway-on-a-Chip, Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, South Korea., Shukla S; Department of Energy and Materials Engineering, Dongguk University, 30 Pildong-ro 1-gil, Seoul 04620, South Korea., Hong SB; Department of Chemical Engineering, Kangwon National University, Samcheok 25913, South Korea., Heo NS; WCSL of Integrated Human Airway-on-a-Chip, Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, South Korea., Bajpai VK; Department of Energy and Materials Engineering, Dongguk University, 30 Pildong-ro 1-gil, Seoul 04620, South Korea., Chun HS; Advanced Food Safety Research Group, BK21 Plus, School of Food Science and Technology, Chung-Ang University, Anseong 17546, South Korea., Jo CH; New Hazardous Substances Team, Department of Food Safety Evaluation, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, 187, Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 28159, South Korea., Choi BG; Department of Chemical Engineering, Kangwon National University, Samcheok 25913, South Korea. Electronic address: bgchoi@kangwon.ac.kr., Huh YS; WCSL of Integrated Human Airway-on-a-Chip, Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, South Korea. Electronic address: yunsuk.huh@inha.ac.kr., Han YK; Department of Energy and Materials Engineering, Dongguk University, 30 Pildong-ro 1-gil, Seoul 04620, South Korea. Electronic address: ykenergy@dongguk.edu. |
| المصدر: | Biosensors & bioelectronics [Biosens Bioelectron] 2018 Jun 01; Vol. 107, pp. 118-122. Date of Electronic Publication: 2018 Feb 07. |
| نوع المنشور: | Evaluation Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Advanced Technology Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989- |
| مواضيع طبية MeSH: | Lab-On-A-Chip Devices*, Gold/*chemistry , Hepatitis B/*blood , Hepatitis B Surface Antigens/*blood , Hepatitis B virus/*isolation & purification , Immunoassay/*instrumentation , Metal Nanoparticles/*chemistry, Antibodies, Immobilized/chemistry ; Equipment Design ; Humans ; Immunoassay/methods ; Limit of Detection ; Metal Nanoparticles/ultrastructure |
| مستخلص: | This study aimed to develop a more sensitive method for the detection of hepatitis B surface antigen (HBsAg) using heteroassembled gold nanoparticles (AuNPs). A single layered localized surface plasmon resonance (LSPR) chip format was developed with antigen-antibody reaction-based detection symmetry using AuNPs, which detected HBsAg at 10 pg/mL. To further improve the detection limit, a modified detection format was fabricated by fixing a secondary antibody (to form a heteroassembled sandwich format) to the AuNP monolayer, which enhanced the detection sensitivity by about 100 times. The developed heteroassembled AuNPs sandwich-immunoassay LSPR chip format was able to detect as little as 100 fg/mL of HBsAg within 10-15 min. In addition, the heteroassembled AuNPs sandwich-immunoassay LSPR chip format did not show any non-specific binding to other tested antigens, including alpha fetoprotein (AFP), C-reactive protein (CRP), and prostate-specific antigen (PSA). These findings confirm that the proposed detection strategy of heteroassembled AuNPs sandwich-immunoassay LSPR chip format may provide a new platform for early diagnosis of various human diseases. (Copyright © 2018 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: HBsAg; Hepatitis B virus; Heteroassembled AuNPs; LSPR chip format; Sandwich-immunoassay |
| المشرفين على المادة: | 0 (Antibodies, Immobilized) 0 (Hepatitis B Surface Antigens) 7440-57-5 (Gold) |
| تواريخ الأحداث: | Date Created: 20180218 Date Completed: 20180910 Latest Revision: 20191210 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.bios.2018.02.019 |
| PMID: | 29454301 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4235 |
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| DOI: | 10.1016/j.bios.2018.02.019 |