دورية أكاديمية
Metaperiodate deglycosylation of Strongyloides venezuelensis larvae: Immunochemical characterization and antigen production for human strongyloidiasis diagnosis.
| العنوان: | Metaperiodate deglycosylation of Strongyloides venezuelensis larvae: Immunochemical characterization and antigen production for human strongyloidiasis diagnosis. |
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| المؤلفون: | Gonzaga HT; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil., Nunes DDS; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil., Ribeiro VDS; Unidade Acadêmica Especial de Biotecnologia, Área de Ciências Biológicas, Universidade Federal de Goiás, Regional Catalão, Av. Dr. Lamartine Pinto Avelar 1120, Bloco M, 75704-020, Catalão, GO, Brazil., Feliciano ND; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil., Cunha-Junior JPD; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil., Costa-Cruz JM; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil. Electronic address: costacruz@ufu.br. |
| المصدر: | Acta tropica [Acta Trop] 2018 Jun; Vol. 182, pp. 27-33. Date of Electronic Publication: 2018 Feb 15. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6254 (Electronic) Linking ISSN: 0001706X NLM ISO Abbreviation: Acta Trop Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Basel. |
| مواضيع طبية MeSH: | Antigens, Helminth/*immunology , Periodic Acid/*metabolism , Strongyloides/*immunology , Strongyloidiasis/*diagnosis, Animals ; Glycosylation ; Humans ; Immunoglobulin G/blood ; Larva/metabolism |
| مستخلص: | Strongyloidiasis is an important helminthiasis affecting million people worldwide. The aim of this study was to use sodium metaperiodate (MP) treatment to immunochemically characterize Strongyloides venezuelensis filariform larvae and use MP-treated heterologous antigen to detect IgG and subclasses in serum. Samples from individuals with definitive diagnosis of strongyloidiasis (n = 50), other parasitic diseases (n = 60) and negative endemic (n = 50) were tested. TG-ROC and two-way ANOVA were applied. MP-treatment resulted on differential localization of carbohydrates at larval structure and no carbohydrate content in saline extract (SE). Electrophoretic profiles were similar before and after treatment. ELISA sensitivity and specificity were: 90%; 88.2% for SE and 92.0%; 94.6% for MP, respectively. When using MP treated antigen we observed reduction in IgG1 and IgG3 detection in strongyloidiasis group and decrease of cross reactions in control groups. Our data demonstrate the role of carbohydrate residues in cross reactions and on the recognition of anti-Strongyloides IgG and its subclasses. (Copyright © 2018 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Deglycosylation; Diagnosis; Sodium metaperiodate; Strongyloides venezuelensis; Strongyloidiasis |
| المشرفين على المادة: | 0 (Antigens, Helminth) 0 (Immunoglobulin G) 10450-60-9 (Periodic Acid) B45A1BUM4Q (metaperiodate) |
| تواريخ الأحداث: | Date Created: 20180219 Date Completed: 20180815 Latest Revision: 20181202 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.actatropica.2018.02.001 |
| PMID: | 29454735 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6254 |
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| DOI: | 10.1016/j.actatropica.2018.02.001 |