دورية أكاديمية
Improved Cytotoxic Effect of Doxorubicin by Its Combination with Sclareol in Solid Lipid Nanoparticle Suspension.
| العنوان: | Improved Cytotoxic Effect of Doxorubicin by Its Combination with Sclareol in Solid Lipid Nanoparticle Suspension. |
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| المؤلفون: | Oliveira MS; Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, CEP 31270-901, Brazil., Lima BHS; Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, CEP 31270-901, Brazil., Goulart GAC; Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, CEP 31270-901, Brazil., Mussi SV; Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, CEP 31270-901, Brazil., Borges GSM; Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, CEP 31270-901, Brazil., Oréfice RL; Department of Metallurgical and Materials Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, CEP 31270-901, Brazil., Ferreira LAM; Department of Pharmaceutics, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, CEP 31270-901, Brazil. |
| المصدر: | Journal of nanoscience and nanotechnology [J Nanosci Nanotechnol] 2018 Aug 01; Vol. 18 (8), pp. 5609-5616. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Scientific Publishers Country of Publication: United States NLM ID: 101088195 Publication Model: Print Cited Medium: Print ISSN: 1533-4880 (Print) Linking ISSN: 15334880 NLM ISO Abbreviation: J Nanosci Nanotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Stevenson Ranch, CA : American Scientific Publishers, 2001-2021 |
| مواضيع طبية MeSH: | Drug Carriers* , Lipids* , Nanoparticles*, Antibiotics, Antineoplastic/*pharmacology , Diterpenes/*chemistry , Doxorubicin/*pharmacology, Animals ; Calorimetry, Differential Scanning ; Humans ; Mice ; Particle Size ; Scattering, Small Angle ; X-Ray Diffraction |
| مستخلص: | This work aims to develop, characterize, and evaluate the anticancer activity of solid lipid nanoparticles (SLN) containing doxorubicin (DOX), an antitumoral from the antracycline class, and sclareol (SC), a lipophilic labdene diterpene (SLN-DOX-SC). The SLN were characterized by Differential Scanning Calorimetry (DSC), X-ray Diffraction (XRD), Small Angle X-ray Diffraction (SAXS), in vitro release, transmission electron microscopy, and polarized light microscopy. Evaluation of cell viability was performed in two cell cultures: MCF-7 (human breast cancer) and 4T1 (murine breast cancer). The SLN showed a size in the range of 128 nm, negative zeta potential, DOX encapsulation efficiency (EE) of 99%, and drug loading (DL) of 66 mg/g. Characterization of the formulation by DSC, XRD, and SAXS revealed the presence of DOX inside the nanoparticles of SLN and suggested increased expulsion/release of this drug when associated with SC. The release profiles revealed that the SLN-DOX-SC showed controlled release of DOX at pH 7.4 with enhanced drug release at low pH, useful for cancer treatment. The SLN-DOX-SC demonstrated to be more effective than the free DOX against 4T1 cells. So, the developed SLN efficiently encapsulate DOX and SC and show good potential as an alternative for cancer treatment. |
| المشرفين على المادة: | 0 (Antibiotics, Antineoplastic) 0 (Diterpenes) 0 (Drug Carriers) 0 (Lipids) 80168379AG (Doxorubicin) B607NP0Q8Y (sclareol) |
| تواريخ الأحداث: | Date Created: 20180221 Date Completed: 20180806 Latest Revision: 20181202 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1166/jnn.2018.15418 |
| PMID: | 29458616 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1533-4880 |
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| DOI: | 10.1166/jnn.2018.15418 |