دورية أكاديمية
أعرض في EDS

scNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells.

التفاصيل البيبلوغرافية
العنوان: scNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells.
المؤلفون: Clark SJ; Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK. stephen.clark@babraham.ac.uk., Argelaguet R; European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, CB10 1SD, UK.; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, CB2 0RE, UK., Kapourani CA; School of Informatics, University of Edinburgh, Scotland, EH8 9AB, UK., Stubbs TM; Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK., Lee HJ; Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK.; Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK.; School of Biomedical Sciences and Pharmacy, The University of Newcastle, Callaghan, NSW, 2308, Australia., Alda-Catalinas C; Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK., Krueger F; Bioinformatics Group, Babraham Institute, Cambridge, CB22 3AT, UK., Sanguinetti G; School of Informatics, University of Edinburgh, Scotland, EH8 9AB, UK., Kelsey G; Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK.; Centre for Trophoblast Research, University of Cambridge, Cambridge, CB2 3EG, UK., Marioni JC; European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, CB10 1SD, UK. marioni@ebi.ac.uk.; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, CB2 0RE, UK. marioni@ebi.ac.uk.; Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK. marioni@ebi.ac.uk., Stegle O; European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, CB10 1SD, UK. oliver.stegle@ebi.ac.uk., Reik W; Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK. wolf.reik@babraham.ac.uk.; Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK. wolf.reik@babraham.ac.uk.; Centre for Trophoblast Research, University of Cambridge, Cambridge, CB2 3EG, UK. wolf.reik@babraham.ac.uk.
المصدر: Nature communications [Nat Commun] 2018 Feb 22; Vol. 9 (1), pp. 781. Date of Electronic Publication: 2018 Feb 22.
نوع المنشور: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Transcription, Genetic*, Chromatin/*metabolism , Embryonic Stem Cells/*metabolism , Nucleosomes/*metabolism , Sequence Analysis, DNA/*methods, Animals ; Cell Differentiation ; DNA Methylation ; Female ; Histones/metabolism ; Male ; Mice ; Nucleosomes/genetics ; Single-Cell Analysis
مستخلص: Parallel single-cell sequencing protocols represent powerful methods for investigating regulatory relationships, including epigenome-transcriptome interactions. Here, we report a single-cell method for parallel chromatin accessibility, DNA methylation and transcriptome profiling. scNMT-seq (single-cell nucleosome, methylation and transcription sequencing) uses a GpC methyltransferase to label open chromatin followed by bisulfite and RNA sequencing. We validate scNMT-seq by applying it to differentiating mouse embryonic stem cells, finding links between all three molecular layers and revealing dynamic coupling between epigenomic layers during differentiation.
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; 22231 United Kingdom CRUK_ Cancer Research UK; MR/K011332/1 United Kingdom MRC_ Medical Research Council
المشرفين على المادة: 0 (Chromatin)
0 (Histones)
0 (Nucleosomes)
تواريخ الأحداث: Date Created: 20180224 Date Completed: 20180501 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC5823944
DOI: 10.1038/s41467-018-03149-4
PMID: 29472610
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-018-03149-4