دورية أكاديمية
Pervasive, Coordinated Protein-Level Changes Driven by Transcript Isoform Switching during Meiosis.
| العنوان: | Pervasive, Coordinated Protein-Level Changes Driven by Transcript Isoform Switching during Meiosis. |
|---|---|
| المؤلفون: | Cheng Z; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA., Otto GM; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA., Powers EN; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA., Keskin A; Department of Biological Sciences, Columbia University, New York, NY 10027, USA., Mertins P; Broad Institute of MIT and Harvard, Cambridge, MA 02136, USA., Carr SA; Broad Institute of MIT and Harvard, Cambridge, MA 02136, USA., Jovanovic M; Department of Biological Sciences, Columbia University, New York, NY 10027, USA. Electronic address: mj2794@columbia.edu., Brar GA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: gabrar@berkeley.edu. |
| المصدر: | Cell [Cell] 2018 Feb 22; Vol. 172 (5), pp. 910-923.e16. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, Ma : Cell Press Original Publication: Cambridge, MIT Press. |
| مواضيع طبية MeSH: | Meiosis/*genetics , Saccharomyces cerevisiae/*genetics , Saccharomyces cerevisiae/*metabolism , Saccharomyces cerevisiae Proteins/*metabolism, Gene Expression Regulation, Fungal ; Genes, Fungal ; Models, Biological ; Molecular Sequence Annotation ; Protein Biosynthesis ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Proteome/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Reproducibility of Results ; Saccharomyces cerevisiae/cytology ; Saccharomyces cerevisiae Proteins/genetics ; Transcription Factors/metabolism |
| مستخلص: | To better understand the gene regulatory mechanisms that program developmental processes, we carried out simultaneous genome-wide measurements of mRNA, translation, and protein through meiotic differentiation in budding yeast. Surprisingly, we observed that the levels of several hundred mRNAs are anti-correlated with their corresponding protein products. We show that rather than arising from canonical forms of gene regulatory control, the regulation of at least 380 such cases, or over 8% of all measured genes, involves temporally regulated switching between production of a canonical, translatable transcript and a 5' extended isoform that is not efficiently translated into protein. By this pervasive mechanism for the modulation of protein levels through a natural developmental program, a single transcription factor can coordinately activate and repress protein synthesis for distinct sets of genes. The distinction is not based on whether or not an mRNA is induced but rather on the type of transcript produced. (Copyright © 2018 Elsevier Inc. All rights reserved.) |
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| معلومات مُعتمدة: | DP2 GM119138 United States GM NIGMS NIH HHS; P50 HG006193 United States HG NHGRI NIH HHS; T32 GM007232 United States GM NIGMS NIH HHS; United States HHMI Howard Hughes Medical Institute |
| فهرسة مساهمة: | Keywords: LUTI; coordination; differentiation; gene expression; isoform; meiosis; ribosome profiling; transcription factor; translation; uORF |
| المشرفين على المادة: | 0 (Protein Isoforms) 0 (Proteome) 0 (RNA, Messenger) 0 (Saccharomyces cerevisiae Proteins) 0 (Transcription Factors) |
| تواريخ الأحداث: | Date Created: 20180224 Date Completed: 20190121 Latest Revision: 20210109 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC5826577 |
| DOI: | 10.1016/j.cell.2018.01.035 |
| PMID: | 29474919 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1097-4172 |
|---|---|
| DOI: | 10.1016/j.cell.2018.01.035 |