دورية أكاديمية
Replacement of soybean oil by fish oil increases cytosolic lipases activities in liver and adipose tissue from rats fed a high-carbohydrate diets.
| العنوان: | Replacement of soybean oil by fish oil increases cytosolic lipases activities in liver and adipose tissue from rats fed a high-carbohydrate diets. |
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| المؤلفون: | Rodrigues AH; Department of Physiology and Biophysics, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Moreira CCL; Department of Physiology and Biophysics, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Neves MJ; Center of Nuclear Technology Development/Brazilian Nuclear Energy Commission (CDTN/CNEN), Belo Horizonte, Minas Gerais, Brazil., Botion LM; Department of Physiology and Biophysics, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Chaves VE; Laboratory of Physiology, Federal University of São João del-Rei, Divinópolis, Minas Gerais, Brazil. Electronic address: valeria.chaves@gmail.com. |
| المصدر: | The Journal of nutritional biochemistry [J Nutr Biochem] 2018 Jun; Vol. 56, pp. 74-80. Date of Electronic Publication: 2018 Feb 02. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 9010081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4847 (Electronic) Linking ISSN: 09552863 NLM ISO Abbreviation: J Nutr Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1996->: New York, NY : Elsevier Science Original Publication: Stoneham, MA, USA : Butterworths, c1990- |
| مواضيع طبية MeSH: | Adipose Tissue/*enzymology , Dietary Carbohydrates/*administration & dosage , Fish Oils/*administration & dosage , Lipase/*metabolism , Liver/*enzymology , Soybean Oil/*administration & dosage, Adipocytes/enzymology ; Animal Feed ; Animals ; Cytosol/enzymology ; Disease Models, Animal ; Epididymis/metabolism ; Fructose/adverse effects ; Glucose Tolerance Test ; Hydrolysis ; Insulin/chemistry ; Lipid Metabolism ; Lipogenesis ; Lipoprotein Lipase/metabolism ; Lipoproteins, VLDL/metabolism ; Male ; Non-alcoholic Fatty Liver Disease/metabolism ; Phosphorylation ; Rats ; Rats, Wistar ; Triglycerides/chemistry ; Triglycerides/metabolism |
| مستخلص: | Several studies have demonstrated that fish oil consumption improves metabolic syndrome and comorbidities, as insulin resistance, nonalcoholic fatty liver disease, dyslipidaemia and hypertension induced by high-fat diet ingestion. Previously, we demonstrated that administration of a fructose-rich diet to rats induces liver lipid accumulation, accompanied by a decrease in liver cytosolic lipases activities. In this study, the effect of replacement of soybean oil by fish oil in a high-fructose diet (FRUC, 60% fructose) for 8 weeks on lipid metabolism in liver and epididymal adipose tissue from rats was investigated. The interaction between fish oil and FRUC diet increased glucose tolerance and decreased serum levels of triacylglycerol (TAG), VLDL-TAG secretion and lipid droplet volume of hepatocytes. In addition, the fish oil supplementation increased the liver cytosolic lipases activities, independently of the type of carbohydrate ingested. Our results firmly establish the physiological regulation of liver cytosolic lipases to maintain lipid homeostasis in hepatocytes. In epididymal adipose tissue, the replacement of soybean oil by fish oil in FRUC diet did not change the tissue weight and lipoprotein lipase activity; however, there was increased basal and insulin-stimulated de novo lipogenesis and glucose uptake. Increased cytosolic lipases activities were observed, despite the decreased basal and isoproterenol-stimulated glycerol release to the incubation medium. These findings suggest that fish oil increases the glycerokinase activity and glycerol phosphorylation from endogenous TAG hydrolysis. Our findings are the first to show that the fish oil ingestion increases cytosolic lipases activities in liver and adipose tissue from rats treated with high-carbohydrate diets. (Copyright © 2018. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Adipose tissue; Cytosolic lipases activities; Fish oil; High-fructose diet; Nonalcoholic fatty liver |
| المشرفين على المادة: | 0 (Dietary Carbohydrates) 0 (Fish Oils) 0 (Insulin) 0 (Lipoproteins, VLDL) 0 (Triglycerides) 0 (very low density lipoprotein triglyceride) 30237-26-4 (Fructose) 8001-22-7 (Soybean Oil) EC 3.1.1.3 (Lipase) EC 3.1.1.34 (Lipoprotein Lipase) |
| تواريخ الأحداث: | Date Created: 20180227 Date Completed: 20190904 Latest Revision: 20190904 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jnutbio.2018.01.010 |
| PMID: | 29481986 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4847 |
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| DOI: | 10.1016/j.jnutbio.2018.01.010 |