دورية أكاديمية
أعرض في EDS

"One-Pot" Sample Processing Method for Proteome-Wide Analysis of Microbial Cells and Spores.

التفاصيل البيبلوغرافية
العنوان: "One-Pot" Sample Processing Method for Proteome-Wide Analysis of Microbial Cells and Spores.
المؤلفون: Swarge BN; Department of Mass Spectrometry of Bio macromolecules, University of Amsterdam, Amsterdam, The Netherlands.; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institutes of Life Sciences, University of Amsterdam, The Netherlands., Roseboom W; Department of Mass Spectrometry of Bio macromolecules, University of Amsterdam, Amsterdam, The Netherlands., Zheng L; Department of Mass Spectrometry of Bio macromolecules, University of Amsterdam, Amsterdam, The Netherlands.; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institutes of Life Sciences, University of Amsterdam, The Netherlands., Abhyankar WR; Department of Mass Spectrometry of Bio macromolecules, University of Amsterdam, Amsterdam, The Netherlands.; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institutes of Life Sciences, University of Amsterdam, The Netherlands., Brul S; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institutes of Life Sciences, University of Amsterdam, The Netherlands., de Koster CG; Department of Mass Spectrometry of Bio macromolecules, University of Amsterdam, Amsterdam, The Netherlands., de Koning LJ; Department of Mass Spectrometry of Bio macromolecules, University of Amsterdam, Amsterdam, The Netherlands.
المصدر: Proteomics. Clinical applications [Proteomics Clin Appl] 2018 Sep; Vol. 12 (5), pp. e1700169. Date of Electronic Publication: 2018 Apr 16.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101298608 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1862-8354 (Electronic) Linking ISSN: 18628346 NLM ISO Abbreviation: Proteomics Clin Appl Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Weinheim : Wiley-VCH, c2007-
مواضيع طبية MeSH: Proteomics*, Bacillus subtilis/*genetics , Proteome/*genetics , Spores, Bacterial/*genetics, Bacillus subtilis/chemistry ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Chromatography, Liquid ; Spores, Bacterial/chemistry ; Tandem Mass Spectrometry
مستخلص: Purpose: Bacterial endospores, the transmissible forms of pathogenic bacilli and clostridia, are heterogeneous multilayered structures composed of proteins. These proteins protect the spores against a variety of stresses, thus helping spore survival, and assist in germination, by interacting with the environment to form vegetative cells. Owing to the complexity, insolubility, and dynamic nature of spore proteins, it has been difficult to obtain their comprehensive protein profiles.
Experimental Design: The intact spores of Bacillus subtilis, Bacillus cereus, and Peptoclostridium difficile and their vegetative counterparts were disrupted by bead beating in 6 m urea under reductive conditions. The heterogeneous mixture was then double digested with LysC and trypsin. Next, the peptide mixture was pre-fractionated with zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) followed by reverse-phase LC-FT-MS analysis of the fractions.
Results: "One-pot" method is a simple, robust method that yields identification of >1000 proteins with high confidence, across all spore layers from B. subtilis, B. cereus, and P. difficile.
Conclusions and Medical Relevance: This method can be employed for proteome-wide analysis of non-spore-forming as well as spore-forming pathogens. Analysis of spore protein profile will help to understand the sporulation and germination processes and to distinguish immunogenic protein markers.
(© 2018 The Authors. Proteomics-Clinical Application Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
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معلومات مُعتمدة: International Netherlands Organization for Scientific Research; International Erasmus Mundus Scholarship program
فهرسة مساهمة: Keywords: Mass spectrometry; One-pot method; Proteomics; Spores; ZIC-HILIC
المشرفين على المادة: 0 (Bacterial Proteins)
0 (Proteome)
تواريخ الأحداث: Date Created: 20180228 Date Completed: 20190403 Latest Revision: 20230928
رمز التحديث: 20230928
مُعرف محوري في PubMed: PMC6174930
DOI: 10.1002/prca.201700169
PMID: 29484825
قاعدة البيانات: MEDLINE
الوصف
تدمد:1862-8354
DOI:10.1002/prca.201700169