دورية أكاديمية
أعرض في EDS

Effects of PACT using phenothiazine-derived drugs and red light on the macrophage x S. aureus interface.

التفاصيل البيبلوغرافية
العنوان: Effects of PACT using phenothiazine-derived drugs and red light on the macrophage x S. aureus interface.
المؤلفون: Santos GMP; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil., de Oliveira SCPS; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil., Monteiro JCS; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil; Department of Biology, Feira de Santana State University, Feira de Santa, BA, 44036-900, Brazil., Fagnani SR; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil., Sampaio FP; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil., Crugeira PJL; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil., Almeida PF; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil; Department of Biointeraction, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA, 40110-100, Brazil., Pinheiro ALB; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil; National Institute of Basic Optics and Applied to Life Sciences, Physics Institute of São Carlos, University of São Paulo, Av. Trabalhador São-Carlense, 400, Parque Arnold Schimidt, São Carlos, SP, 13566-590, Brazil; Biomedical Engineering Institute, Universidade Brasil, São Paulo, SP, 08230-030, Brazil. Electronic address: albp@ufba.br.
المصدر: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2018 Jun; Vol. 22, pp. 96-100. Date of Electronic Publication: 2018 Feb 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101226123 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-1597 (Electronic) Linking ISSN: 15721000 NLM ISO Abbreviation: Photodiagnosis Photodyn Ther Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam ; Boston : Elsevier, c2004-
مواضيع طبية MeSH: Macrophages/*drug effects , Phenothiazines/*pharmacology , Photochemotherapy/*methods , Photosensitizing Agents/*pharmacology , Staphylococcus aureus/*drug effects, Light ; Macrophages/microbiology ; Methylene Blue/pharmacology ; Microscopy, Fluorescence ; Tolonium Chloride/pharmacology
مستخلص: The aim of this study was to evaluate the lethal potential of macrophages infected with Staphylococcus aureus after PACT (Photochemical Antimicrobial Chemotherapy) using phenothiazine derivatives (a solution containing 1:1 methylene blue and O toluidine blue) and laser (660 nm, 40 mW, 60 s, 12 J/cm 2 ) or LED (632 ± 2 nm, 145 mW, 40 s, 12 J/cm 2 ). Six experimental groups were evaluated: Control Group (untreated); Photosensitizer group (phenothiazines - 12.5 μg/mL); Laser Group; LED Group; Laser PACT Group; and LED PACT Group. The pre-irradiation time used in this study was 5 min. Macrophages and bacteria were cultured in specific culture media and/or allowed interaction between the cell types. Subsequently, tests were carried out to evaluate microbial proliferation, ROS production by macrophages and survival capacity of S. aureus after phagocytosis. Fluorescence microscopy assays were performed with the H 2 DCFDA probe, after PACT, at the initial time (0 h), 4-h and 12-h. The tests were performed in triplicate and the statistical test used was ANOVA with Tukey post-test. After PACT, a statistically significant difference (p > 0.0001) was observed between the microbial growth of the control group and the PACTs groups. Laser PACT and LED PACT groups presented, respectively, reductions of 84.2% and 81.5% when compared to control and 53.3% and 46% when compared to the photosensitizer group. It is concluded that the therapeutic protocols presented in this study increased the phagocytic capacity, the response rate of the phagocytes and the consequent reduction of the numbers of S. aureus for both PACT protocols, however the increase in ROS production was only observed in the group irradiated with Laser light.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: LED; Laser; Macrophages; PACT
المشرفين على المادة: 0 (Phenothiazines)
0 (Photosensitizing Agents)
15XUH0X66N (Tolonium Chloride)
T42P99266K (Methylene Blue)
تواريخ الأحداث: Date Created: 20180303 Date Completed: 20181105 Latest Revision: 20181105
رمز التحديث: 20231215
DOI: 10.1016/j.pdpdt.2018.02.022
PMID: 29499391
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-1597
DOI:10.1016/j.pdpdt.2018.02.022