دورية أكاديمية
أعرض في EDS

Comparative analysis of Mouse Inoculation Test and Virus Isolation in Cell Culture for rabies diagnosis in animals of Parana, Brazil.

التفاصيل البيبلوغرافية
العنوان: Comparative analysis of Mouse Inoculation Test and Virus Isolation in Cell Culture for rabies diagnosis in animals of Parana, Brazil.
المؤلفون: Corona TF; Divisão de Zoonoses, Divisão de Epidemiologia e Laboratórios de Controle de Doenças, Laboratório Central do Estado do Paraná, Curitiba, PR, Brasil.; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Paraná, Curitiba, PR, Brasil., Böger B; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Paraná, Curitiba, PR, Brasil., Rocha TCD; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Paraná, Curitiba, PR, Brasil., Svoboda WK; Instituto Latino-Americano de Ciências da Vida e da Natureza, Universidade Federal da Integração Latino-Americana, Foz do Iguaçu, PR, Brasil., Gomes EC; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Paraná, Curitiba, PR, Brasil.
المصدر: Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2018 Jan-Feb; Vol. 51 (1), pp. 39-43.
نوع المنشور: Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Sociedade Brasileira de Medicina Tropical Country of Publication: Brazil NLM ID: 7507456 Publication Model: Print Cited Medium: Internet ISSN: 1678-9849 (Electronic) Linking ISSN: 00378682 NLM ISO Abbreviation: Rev Soc Bras Med Trop Subsets: MEDLINE
أسماء مطبوعة: Publication: <1997-> : Uberaba, MG, Brazil : Sociedade Brasileira de Medicina Tropical
Original Publication: Rio De Janeiro : Sociedade Brasileira De Medicina Tropical
مواضيع طبية MeSH: Bioethical Issues* , Containment of Biohazards* , Occupational Health*, Cell Culture Techniques/*methods , Fluorescent Antibody Technique/*methods , Rabies/*diagnosis , Rabies virus/*immunology, Animal Welfare ; Animals ; Cell Culture Techniques/economics ; Costs and Cost Analysis ; Fluorescent Antibody Technique/economics ; Mice ; Rabies virus/isolation & purification ; Reproducibility of Results ; Risk Factors ; Sensitivity and Specificity
مستخلص: Introduction: Rabies is an acute zoonotic disease, caused by a rhabdovirus that can affect all mammals, and is commonly transmitted by the bite of a rabid animal. The definitive diagnosis is laboratorial, by the Fluorescent Antibody Test (FAT) as a quick test and Mouse Inoculation Test (MIT) as a confirmatory test (gold standard). Studies conducted over the past three decades indicate that MIT and Virus Isolation in Cell Culture (VICC) can provide the same effectiveness, the latter being considered superior in bioethics and animal welfare. The aim of this study was to compare VICC with MIT, in terms of accuracy, biosafety and occupational health, supply and equipment costs, bioethics and animal welfare, in a Brazilian public health lab.
Methods: We utilized 400 samples of animal neurological tissue to compare the performance of VICC against MIT. The variables analyzed were accuracy, biosafety and occupational health, time spent in performing the tests, supply and equipment costs, bioethics and animal welfare evaluation.
Results: Both VICC and MIT had almost the same accuracy (99.8%), although VICC presented fewer risks regarding biosafety and mental health of the technicians, and reduced time between inoculation and obtaining the results (approximately 22 days less). In addition, VICC presented lower supply costs (86.5% less), equipment costs (32.6% less), and the advantage of not using animals.
Conclusions: These results confirm that VICC can replace MIT, offering the same accuracy and better features regarding cost, results, biosafety and occupational health, and bioethics and animal welfare.
تواريخ الأحداث: Date Created: 20180308 Date Completed: 20180419 Latest Revision: 20180419
رمز التحديث: 20231215
DOI: 10.1590/0037-8682-0303-2017
PMID: 29513840
قاعدة البيانات: MEDLINE
الوصف
تدمد:1678-9849
DOI:10.1590/0037-8682-0303-2017