Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus.

التفاصيل البيبلوغرافية
العنوان:	Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus.
المؤلفون:	Gonçalves FZ; Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil., Lizarte Neto FS; Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil., Novais PC; Departamento de Ciências da Saúde, Universidade de Marília, Marília, SP, Brasil., Gattas D; Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil., Lourenço LG; Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil., de Carvalho CAM; Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Maceio, AL, Brasil., Tirapelli DPC; Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil., Molina CAF; Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil., Tirapelli LF; Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil., Tucci S Jr; Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
المصدر:	Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas [Braz J Med Biol Res] 2018 Mar 01; Vol. 51 (3), pp. e6329. Date of Electronic Publication: 2018 Mar 01.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Associação Brasileira de Divulgação Científica Country of Publication: Brazil NLM ID: 8112917 Publication Model: Electronic Cited Medium: Internet ISSN: 1414-431X (Electronic) Linking ISSN: 0100879X NLM ISO Abbreviation: Braz J Med Biol Res Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: [SP, Brasil : Associação Brasileira de Divulgação Científica, 1981-
مواضيع طبية MeSH:	Alcoholism/metabolism , Diabetes Mellitus, Experimental/metabolism , Endothelin-1/analysis , MicroRNAs/analysis , Penis/metabolism , Receptor, Endothelin A/analysis , Receptor, Endothelin B/*analysis, Alcoholism/complications ; Alcoholism/physiopathology ; Animals ; Diabetes Mellitus, Experimental/complications ; Diabetes Mellitus, Experimental/physiopathology ; Immunohistochemistry ; Male ; Penis/physiopathology ; Rats ; Rats, Wistar
مستخلص:	Recent evidence shows that chronic ethanol consumption increases endothelin (ET)-1 induced sustained contraction of trabecular smooth muscle cells of the corpora cavernosa in corpus cavernosum of rats by a mechanism that involves increased expression of ETA and ETB receptors. Our goal was to evaluate the effects of alcohol and diabetes and their relationship to miRNA-155, miRNA-199 and endothelin receptors in the corpus cavernosum and blood of rats submitted to the experimental model of diabetes mellitus and chronic alcoholism. Forty-eight male Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D), and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study the protein expression of endothelin receptors by immunohistochemistry and expression of miRNAs-155 and -199 in serum and the cavernous tissue. Immunostaining for endothelin receptors was markedly higher in the A, D, and AD groups than in the C group. Moreover, a significant hypoexpression of the miRNA-199 in the corpus cavernosum tissue from the AD group was observed, compared to the C group. When analyzing the microRNA profile in blood, a significant hypoexpression of miRNA-155 in the AD group was observed compared to the C group. The miRNA-199 analysis demonstrated significant hypoexpression in D and AD groups compared to the C group. Our findings in corpus cavernosum showed downregulated miRNA-155 and miRNA-199 levels associated with upregulated protein expression and unaltered mRNA expression of ET receptors suggesting decreased ET receptor turnover, which can contribute to erectile dysfunction in diabetic rats exposed to high alcohol levels.
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المشرفين على المادة:	0 (Endothelin-1) 0 (MicroRNAs) 0 (Receptor, Endothelin A) 0 (Receptor, Endothelin B)
تواريخ الأحداث:	Date Created: 20180308 Date Completed: 20180423 Latest Revision: 20181114
رمز التحديث:	20231215
مُعرف محوري في PubMed:	PMC5912101
DOI:	10.1590/1414-431X20176329
PMID:	29513879
قاعدة البيانات:	MEDLINE

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تدمد:	1414-431X
DOI:	10.1590/1414-431X20176329