دورية أكاديمية
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Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes.

التفاصيل البيبلوغرافية
العنوان: Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes.
المؤلفون: Garcia Ribeiro RS; Biomedical MRI/MoSAIC, Department of Imaging and Pathology, University of Leuven, Herestraat 49, B3000 Leuven, Belgium. Rita.Sofia.Garcia.Ribeiro@vub.ac.be., Ketkar-Atre A; Biomedical MRI/MoSAIC, Department of Imaging and Pathology, University of Leuven, Herestraat 49, B3000 Leuven, Belgium., Yin T; Biomedical MRI/MoSAIC, Department of Imaging and Pathology, University of Leuven, Herestraat 49, B3000 Leuven, Belgium. yinting527@gmail.com.; Laboratory for Functional and Metabolic Imaging, Ecole Polytechnique Federale Lausanne, 1015 Lausanne, Switzerland. yinting527@gmail.com., Louchami K; Laboratory of Experimental Hormonology, ULB, Campus Erasme, Route de Lennik 808, B1070 Brussels, Belgium. karim.louchami@ulb.ac.be., Struys T; Laboratory of Histology, Biomedical Research Institute, Hasselt University, Campus Diepenbeek, Agoralaan, B3590 Diepenbeek, Belgium. tom.struys@sprofit.com., Lambrichts I; Laboratory of Histology, Biomedical Research Institute, Hasselt University, Campus Diepenbeek, Agoralaan, B3590 Diepenbeek, Belgium. ivo.lambrichts@uhasselt.be., Sener A; Laboratory of Experimental Hormonology, ULB, Campus Erasme, Route de Lennik 808, B1070 Brussels, Belgium. abdsener@ulb.ac.be., Malaisse WJ; Department of Biochemistry, ULB, Campus Erasme, Route de Lennik 808, B1070 Brussels, Belgium. malaisse@ulb.ac.be., De Cuyper M; Laboratory of BioNanoColloids, Interdisciplinary Research Centre, University of Leuven, Etienne Sabbelaan 53, B8500 Kortrijk, Belgium. marcel.decuyper@kuleuven.be., Himmelreich U; Biomedical MRI/MoSAIC, Department of Imaging and Pathology, University of Leuven, Herestraat 49, B3000 Leuven, Belgium. Uwe.Himmelreich@med.kuleuven.be.
المصدر: Journal of personalized medicine [J Pers Med] 2018 Mar 12; Vol. 8 (1). Date of Electronic Publication: 2018 Mar 12.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101602269 Publication Model: Electronic Cited Medium: Print ISSN: 2075-4426 (Print) Linking ISSN: 20754426 NLM ISO Abbreviation: J Pers Med Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, 2011-
مستخلص: Pancreatic islets (PIs) transplantation is an alternative approach for the treatment of severe forms of type 1 diabetes (T1D). To monitor the success of transplantation, it is desirable to follow the location of engrafted PIs non-invasively. In vivo magnetic resonance imaging (MRI) of transplanted PIs is a feasible cell tracking method; however, this requires labeling with a suitable contrast agent prior to transplantation. We have tested the feasibility of cationic magnetoliposomes (MLs), compared to commercial contrast agents (Endorem and Resovist), by labeling insulinoma cells and freshly isolated rat PIs. It was possible to incorporate Magnetic Ressonance (MR)-detectable amounts of MLs in a shorter time (4 h) when compared to Endorem and Resovist. MLs did not show negative effects on the PIs' viability and functional parameters in vitro. Labeled islets were transplanted in the renal sub-capsular region of healthy mice. Hypointense contrast in MR images due to the labeled PIs was detected in vivo upon transplantation, while MR detection of PIs labeled with Endorem and Resovist was only possible after the addition of transfection agents. These findings indicate that MLs are suitable to image PIs, without affecting their function, which is promising for future longitudinal pre-clinical and clinical studies involving the assessment of PI transplantation.
Competing Interests: The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.
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فهرسة مساهمة: Keywords: MRI; SPIOs; cell imaging; contrast agents; diabetes mellitus; insulin; islet transplantation; magnetoliposomes; pancreatic islets; transmission electron microscopy
تواريخ الأحداث: Date Created: 20180315 Latest Revision: 20220410
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5872086
DOI: 10.3390/jpm8010012
PMID: 29534555
قاعدة البيانات: MEDLINE
الوصف
تدمد:2075-4426
DOI:10.3390/jpm8010012